A5067846615- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Animal Genetics and Reproduction
- Prostate Cancer Treatment and Research
- Fish Ecology and Management Studies
- Sarcoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- Cell Adhesion Molecules Research
- Gastrointestinal Tumor Research and Treatment
- Congenital heart defects research
- Neuroblastoma Research and Treatments
- Marine and fisheries research
- Zebrafish Biomedical Research Applications
- Protease and Inhibitor Mechanisms
- Hematopoietic Stem Cell Transplantation
- Protein Degradation and Inhibitors
- Reproductive System and Pregnancy
Cornell University
2010-2023
Weill Cornell Medicine
2019-2023
NewYork–Presbyterian Hospital
2014
Memorial Sloan Kettering Cancer Center
2005-2010
Howard Hughes Medical Institute
2007-2010
Stem Cell Institute
2008
Scripps Research Institute
2001-2005
American Red Cross
2005
State University of New York
2001
Evidence of male-to-female sexual transmission Zika virus (ZIKV) and viral RNA in semen sperm months after infection supports a potential role for testicular cells ZIKV propagation. Here, we demonstrate that germ (GCs) are most susceptible to ZIKV. We found only GCs infected by ZIKV, but not those dengue yellow fever virus, produce high levels infectious virus. This observation coincides with decreased expression interferon-stimulated gene Ifi44l ZIKV-infected GCs, overexpression results...
Vascular cells contribute to organogenesis and tumorigenesis by producing unknown factors. Primary endothelial (PECs) provide an instructive platform for identifying factors that support stem cell tumor homeostasis. However, long-term maintenance of PECs requires stimulation with cytokines serum, resulting in loss their angiogenic properties. To circumvent this hurdle, we have discovered the adenoviral E4ORF1 gene product maintains survival facilitates organ-specific purification PECs, while...
We have demonstrated previously that new blood vessel formation induced by angiogenic growth factors in onplants placed on the chorioallantoic membrane (CAM) of chick embryos is critically dependent cleavage fibrillar collagen a unidentified interstitial collagenase. In present study we used quantitative CAM angiogenesis system to search for and functionally characterize host avian collagenases responsible remodeling associated with angiogenesis. Among matrix metalloproteinases (MMPs)...
We examined, using underwater snorkeling observations and field experiments, the influence of food availability on foraging behavior, habitat use, survival age-0 Atlantic salmon (Salmo salar) during critical first-summer growth period. While most feeding attempts were directed at drifting invertebrate prey, we found a higher rate benthic forays than previously reported for salmon. Greater abundance was associated with foray rates, more time allocated to foraging, occupancy microhabitat...
Hematogenous metastasis accounts for the majority of cancer-related deaths, yet mechanism remains unclear. Circulating tumor cells (CTCs) in blood may employ different pathways to cross endothelial barrier and establish a metastatic niche. Several studies provide evidence that prostate cancer (PCa) cell tethering rolling on microvascular endothelium via E-selectin/E-selectin ligand interactions under shear flow theoretically promote extravasation contribute development metastases. However,...
Abstract Spermatogonial stem and progenitor cells (SSCs) generate adult male gametes. During in vitro expansion, these unipotent murine spontaneously convert to multipotent spermatogonial-derived (MASCs). Here we investigate this conversion process through integrative transcriptomic epigenomic analyses. We find SSCs that promoters essential maintenance differentiation of embryonic (ESCs) are enriched with histone H3-lysine4 -lysine 27 trimethylations. These bivalent modifications maintained...
Summary The optimal markers for human spermatogonial stem cells ( SSC s) are not known. Among the genes recently linked to s in mice and other animals basic helix‐loop‐helix transcription factor ID 4 orphan G‐protein‐coupled receptor GPR 125. While 125 considered putative s, they have been evaluated coexpression tissue. Furthermore, neither size nor character of populations that express 125, respectively, A major barrier addressing these questions is availability healthy adult testis tissue...
The undifferentiated spermatogonia of adult mouse testes are composed both true stem cells and committed progenitors. It is unclear what normally prevents these germ from manifesting multipotency. critical elements the spermatogonial cell niche, while poorly understood, thought to be Sertoli with several other somatic types in close proximity. We recently discovered a novel orphan G-protein coupled receptor (GPR125) that restricted within testis. GPR125 expression was maintained when...
Pathogenic de novo mutations increase with fathers' age and could be amplified through competition between genetically distinct subpopulations of spermatogonial stem cells (SSCs). Here, we tested the fitness SSCs bearing wild-type human FGFR2 or an Apert syndrome mutant, (S252W), to provide experimental evidence for SSC competition. The S252W allele conferred enhanced FGFR2-mediated signaling, particularly at very low concentrations ligand, also subtle changes in gene expression. Mutant...
Abstract Stem cells reside in specialized microenvironments created by supporting stromal that orchestrate self-renewal and lineage-specific differentiation. However, the precise identity of cellular molecular pathways support stem is not known. For example, long-term culture prototypical cells, such as adult spermatogonial progenitor (SPCs), vitro has been impeded lack an optimal cell line initiates sustains proliferation these cells. Indeed, current methods, including use mouse embryonic...
Loss of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) expression by CpG promoter hypermethylation is associated with metastasis in gastroenteropancreatic neuroendocrine tumors; however, the mechanism how UCHL1 loss contributes to metastatic potential remains unclear. In this study, we first confirmed that on immunohistochemistry was significantly tumors a translational pancreatic tumor (PNET) cohort, sensitivity and specificity 78% 89%, respectively. To study driving aggressive phenotype,...
Abstract Germline stem and progenitor cells can be extracted from the adult mouse testis maintained long-term in vitro. Yet, optimal culture conditions for preserving cell activity are unknown. Recently, multiple members of Eph receptor family were detected murine spermatogonia, but their roles remain obscure. One such gene, Ephb2, is crucial maintenance somatic was previously found enriched at level mRNA spermatogonia. We Ephb2 protein primary spermatogonial cultures hypothesized that plays...