A5101614223- Marine Sponges and Natural Products
- MicroRNA in disease regulation
- Microbial Natural Products and Biosynthesis
- RNA Research and Splicing
- Synthesis and Biological Activity
- Cell Adhesion Molecules Research
- Hematopoietic Stem Cell Transplantation
- Protein Kinase Regulation and GTPase Signaling
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- Angiogenesis and VEGF in Cancer
- Glutathione Transferases and Polymorphisms
- Medical Imaging Techniques and Applications
- Synthetic Organic Chemistry Methods
- DNA Repair Mechanisms
- RNA regulation and disease
- Oral microbiology and periodontitis research
- Neurofibromatosis and Schwannoma Cases
- Enzyme Production and Characterization
- Chemical Synthesis and Analysis
- Acute Myeloid Leukemia Research
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Chronic Myeloid Leukemia Treatments
Shimane University
2025
Howard Hughes Medical Institute
2008-2023
Rockefeller University
2017-2023
Chiba University
1997-2023
The University of Tokyo
2022
Toranomon Hospital
2016-2020
St. Marianna University School of Medicine
2017
Cleveland Clinic Lerner College of Medicine
2012
Fistula Foundation
2012
Showa University
2011
Vascular cells contribute to organogenesis and tumorigenesis by producing unknown factors. Primary endothelial (PECs) provide an instructive platform for identifying factors that support stem cell tumor homeostasis. However, long-term maintenance of PECs requires stimulation with cytokines serum, resulting in loss their angiogenic properties. To circumvent this hurdle, we have discovered the adenoviral E4ORF1 gene product maintains survival facilitates organ-specific purification PECs, while...
How type I and II interferons prevent periodic reemergence of latent pathogens in tissues diverse cell types remains unknown. Using homogeneous neuron cultures latently infected with herpes simplex virus 1, we show that extrinsic or interferon acts directly on neurons to induce unique gene expression signatures inhibit the reactivation-specific burst viral genome-wide transcription called phase I. Surprisingly, suppressed reactivation only during a limited period early preceding productive...
The assembly of the mammalian brain is orchestrated by temporally coordinated waves gene expression. Post-transcriptional regulation microRNAs (miRNAs) a key aspect this program. Indeed, deletion neuron-enriched miRNAs induces strong developmental phenotypes, and miRNA levels are altered in patients with neurodevelopmental disorders. However, mechanisms used to instruct development remain largely unexplored. Here, we identified miR-218 as critical regulator hippocampal assembly. MiR-218...
We herein report a 56-year-old man with severe hypocalcemia during ruxolitinib therapy for myelofibrosis transitioning from JAK2 mutation-positive polycythemia vera. Blood transfusions were administered every one to two weeks ruxolitinib-induced anemia. tests revealed low TRACP-5b, 25-hydroxyvitamin D (25 (OH) D), and 1,25-dihydroxyvitamin D3 (1,25 2 D3) levels within the lower reference range. Intact-PTH relatively compared calcium levels. Severe is rare may be caused by combination of...
Murayama R, Kobayashi M, Takeshita A, Yasui T, Yamamoto M. MAPKs, activator protein-1 and nuclear factor-κB mediate production of interleukin-1β-stimulated cytokines, prostaglandin E 2 MMP-1 in human periodontal ligament cells. J Periodont Res 2011; 46: 568–575. © 2011 John Wiley & Sons A/S Background Objective: Determination the interleukin-1 (IL-1) signaling cascades that lead to various inflammatory mediators catabolic factors may clarify attractive targets for therapeutic intervention...
A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80 degrees C requires Mg2+ or Mn2+ for activity. Tth111II cleaves phi X174RFDNA into 11 fragments lambda NA more than 25 fragments. From the 5'-terminal sequences of TthlllII determined by two dimensional homochromatography survey on nucleotide sequence X174RFDNA, it was concluded that recognizes DNA (see former index)...
Post-transcriptional regulation by microRNAs (miRNAs) is essential for complex molecular responses to physiological insult and disease. Although many disease-associated miRNAs are known, their global targets culminating network effects on pathophysiology remain poorly understood. We applied Argonaute (AGO) crosslinking immunoprecipitation (CLIP) systematically elucidate altered miRNA-target interactions in brain following ischemia reperfusion (I/R) injury. Among 1,190 identified, the most...
Telomerase reverse transcriptase (TERT) promoter mutation is the most frequent genetic alteration in hepatocellular carcinoma (HCC). However, there currently no suitable highly sensitive method that can detect such using serum cell-free DNA (cfDNA). We analyzed somatic point mutations substitute cytosine for thymidine at position 228 (C228T), as one of hotspots TERT mutations, cfDNA a detection wild-type blocking polymerase chain reaction (WTB-PCR) combined with Sanger sequencing. In...
Objective To investigate the impact of different tracer modifications on imaging cancer metabolism, focusing comparison fluorescent glucose-analog tracers (2-NBDG and 2-DG-750) radiolabeled 18F-FDG in both in-vitro in-vivo settings. Methods We conducted an comparative study using four cell lines, each with unique glucose uptake characteristics. The involved direct three tracers: 2-NBDG, 2-DG-750 18F-FDG, examining their internalization behaviors, metabolic functionality localization effects...
We previously reported that Thermus thermophilus 111 contained two restriction enzymes, Tth111 I and II. The former does not cleave phi X174RFDNA the latter does. have now found another endonuclease activity able to in cell extract of T. 111. protein with this was purified a homogeneous state by chromatography on cellulose phosphate, heparin-Sepharose 4B hydroxylapatite, successively. However, always accompanied during purification procedure also showed strong activity, suggesting...