A5023339605- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Biosimilars and Bioanalytical Methods
- Bacterial Infections and Vaccines
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Circular RNAs in diseases
- Protease and Inhibitor Mechanisms
- Neonatal Respiratory Health Research
- Nanopore and Nanochannel Transport Studies
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cancer Research and Treatments
- Inflammasome and immune disorders
- Amoebic Infections and Treatments
- Intensive Care Unit Cognitive Disorders
- Pneumonia and Respiratory Infections
- Tryptophan and brain disorders
University of Gothenburg
2015-2023
Pohang University of Science and Technology
2011-2016
Jagiellonian University
2014
University Medical Center
2014
AGH University of Krakow
2014
Exosomes, the endogenous nanocarriers that can deliver biological information between cells, were recently introduced as new kind of drug delivery system. However, mammalian cells release relatively low quantities exosomes, and purification exosomes is difficult. Here, we developed bioinspired exosome-mimetic nanovesicles chemotherapeutics to tumor tissue after systemic administration. The chemotherapeutics-loaded produced by breakdown monocytes or macrophages using a serial extrusion...
Secretion of extracellular vesicles is a general cellular activity that spans the range from simple unicellular organisms (e.g. archaea; Gram-positive and Gram-negative bacteria) to complex multicellular ones, suggesting this vesicle-mediated communication evolutionarily conserved. Extracellular are spherical bilayered proteolipids with mean diameter 20-1,000 nm, which known contain various bioactive molecules including proteins, lipids, nucleic acids. Here, we present EVpedia, an integrated...
The isolation of extracellular vesicles (EVs) from blood is great importance to understand the biological role circulating EVs and develop as biomarkers disease. Due concurrent presence lipoprotein particles, however, one most difficult body fluids isolate from. aim this study was a robust method characterise with minimal contamination by plasma proteins particles. Plasma serum were collected healthy subjects, isolated size-exclusion chromatography (SEC), particles being present in fractions...
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Extracellular vesicles are released by various cell types, particularly tumor cells, and may be potential targets for blood-based cancer diagnosis. However, studies performed on blood-borne to date have been limited lack of effective, standardized purification strategies. Using in situ prepared nanoporous membranes, we present a simple strategy employing microfluidic filtration system isolate from whole blood samples. This method can applied purify nano-sized particles allowing isolation...
// Valentina R. Minciacchi 1 , Sungyong You Cristiana Spinelli Samantha Morley 2 Mandana Zandian Paul-Joseph Aspuria 3 Lorenzo Cavallini 1,4 Chiara Ciardiello 1,5 Mariana Reis Sobreiro Matteo Morello Geetanjali Kharmate 6 Su Chul Jang 7 Dae-Kyum Kim Elham Hosseini-Beheshti Emma Tomlinson Guns Martin Gleave Yong Song Gho Suresh Mathivanan 8 Wei Yang Michael Freeman 1,2 and Dolores Di Vizio Division of Cancer Biology Therapeutics, Departments Surgery, Biomedical Sciences Pathology Laboratory...
Extracellular vesicles (EVs) are an important intercellular communication system facilitating the transfer of macromolecules between cells. Delivery exogenous cargo tethered to EV surface or packaged inside lumen key strategies for generating therapeutic EVs. We identified two "scaffold" proteins, PTGFRN and BASP1, that preferentially sorted into EVs enable high-density display luminal loading a wide range molecules, including cytokines, antibody fragments, RNA binding vaccine antigens,...
Abstract Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances biomedical applications of exosomes, it is crucial understand vivo distribution and behavior exosomes. Here, we developed a simple method for radiolabeling macrophage-derived exosome-mimetic nanovesicles (ENVs) with 99m Tc-HMPAO under physiologic conditions monitored Tc-HMPAO-ENVs using SPECT/CT living mice. ENVs were produced...
Abstract Gut microbes might influence host metabolic homeostasis and contribute to the pathogenesis of type 2 diabetes (T2D), which is characterized by insulin resistance. Bacteria-derived extracellular vesicles (EVs) have been suggested be important in diseases once believed non-infectious. Here, we hypothesize that gut microbe-derived EVs are T2D. In vivo administration stool from high fat diet (HFD)-fed mice induced resistance glucose intolerance compared regular (RD)-fed mice....
The majority of extracellular vesicle (EV) studies conducted to date have been performed on cell lines with little knowledge how well these represent the characteristics EVs in vivo. aim this study was establish a method isolate and categorize subpopulations isolated directly from tumour tissue. First we established an isolation protocol for metastatic melanoma tissue, which included enzymatic treatment (collagenase D DNase). Small large were differential ultracentrifugation, further...
Effectiveness of checkpoint immunotherapy in cancer can be undermined by immunosuppressive tumor-associated macrophages (TAMs) with an M2 phenotype. Reprogramming TAMs toward a proinflammatory M1 phenotype is novel approach to induce antitumor immunity. The controlled key transcription factors such as signal transducer and activator 6 (STAT6), which have been “undruggable” selectively TAMs. We describe engineered exosome therapeutic candidate delivering antisense oligonucleotide (ASO)...
Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing recipient cell's phenotype. While role of RNAs in EVs has been extensively studied, function DNA remains elusive. Here, we distinguished novel heterogeneous subpopulations small extracellular (sEVs) based on their content topology. Low- high-density sEV subsets from a human mast cell line (HMC-1) an erythroleukemic (TF-1) were separated using high-resolution iodixanol...
Extracellular vesicles (EVs), including exosomes and microvesicles, are secreted from all cells, convey messages between cells in health disease. However, the diversity of EV subpopulations is only beginning to be explored. Since EVs have been implicated tumour microenvironmental communication, we started determine specifically this tissue. To do this, isolated directly patient melanoma metastatic tissues. Using membrane isolation mass spectrometry analysis, discovered enrichment...
Abstract Cyclic dinucleotide (CDN) agonists of the STimulator InterferoN Genes (STING) pathway have shown immune activation and tumor clearance in pre-clinical models. However, CDNs administered intratumorally also promote STING leading to direct cytotoxicity many cell types microenvironment (TME), systemic inflammation due rapid extravasation CDN, ablation TME. These result a failure establish immunological memory. ExoSTING, an engineered extracellular vesicle (EV) exogenously loaded with...
Melanoma cells release different types of extracellular vesicles (EVs) into the milieu that are involved with communication and signaling in tumor microenvironment. Subsets EVs include exosomes, microvesicles, apoptotic bodies carry protein genetic (RNA) cargos. To define contribution RNA cargo melanoma cell derived we performed small sequencing to identify RNAs EV subsets. Using validated centrifugation protocols, separated these subsets released by line MML-1, Ion Torrent platform....
The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator low survival rate. Various cancer cells, including those colorectal (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation ascites. Although recent progress has revealed that play multiple roles tumor progression, protein composition pathological function ascites-derived are still unknown. Here, we report first global proteomic analyses highly purified derived from...
Abstract Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate topology found in EVs, as this can fundamentally influence biological effects EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows many proteins cytosolic origin localized on surface detailed analysis EV proteome at peptide level revealed a number...
Evaluation of kinetic distribution and behaviors nanoparticles in vivo provides crucial clues into their roles living organisms. Extracellular vesicles are evolutionary conserved nanoparticles, known to play important biological functions intercellular, inter-species, inter-kingdom communication. In this study, the first analysis biodistribution outer membrane (OMVs)—bacterial extracellular vesicles—with immune-modulatory is performed. OMVs, injected intraperitoneally, spread whole mouse...
Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that released mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The form TGFβ-1 is associated with the via heparinase-II pH-sensitive elements. These traffic endocytic compartment human mesenchymal stem (MSCs) within 60 min exposure....
Artificial exosomes of ~100 nm diameter, enclosed with lipid bilayers, are fabricated from living cells and transfer biological signal components such as encapsulated RNAs proteins, plasma membrane or both.
Cancer cells actively release extracellular vesicles (EVs), including exosomes and microvesicles, into surrounding tissues. These EVs play pleiotropic roles in cancer progression metastasis, invasion, angiogenesis, immune modulation. However, the proteomic differences between primary metastatic cell‐derived remain unclear. Here, we conducted comparative analysis derived from human colorectal (SW480) their derivatives (SW620). Using label‐free quantitation, identified 803 787 proteins SW480...
Sepsis remains a source of high mortality in hospitalized patients despite proper antibiotic approaches. Encouragingly, mesenchymal stromal cells (MSCs) and their produced extracellular vesicles (EVs) have been shown to elicit anti-inflammatory effects multiple inflammatory conditions including sepsis. However, EVs are generally released from mammalian relatively low amounts, high-yield isolation is still challenging due complicated procedure. To get over these limitations, very similar can...
The promise of IL12 as a cancer treatment has yet to be fulfilled with multiple tested approaches being limited by unwanted systemic exposure and unpredictable pharmacology. To address these limitations, we generated exoIL12, novel, engineered exosome therapeutic that displays functional on the surface an exosome. exosomal expression was achieved via fusion abundant protein PTGFRN resulting in equivalent potency vitro recombinant (rIL12) demonstrated IFNγ production. Following intratumoral...