A5070498464- Caveolin-1 and cellular processes
- Cancer, Lipids, and Metabolism
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Advanced Proteomics Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Metabolomics and Mass Spectrometry Studies
- Prostate Cancer Treatment and Research
- Metabolism, Diabetes, and Cancer
- Esophageal Cancer Research and Treatment
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Multiple Myeloma Research and Treatments
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Galectins and Cancer Biology
- Mass Spectrometry Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Hypoxia, and Metabolism
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Immune Cell Function and Interaction
- Cancer survivorship and care
QIMR Berghofer Medical Research Institute
2018-2025
The University of Queensland
2015-2024
Riverside Methodist Hospital
2022-2024
American College of Sports Medicine
2023
St Andrew's Healthcare
2021-2022
Translational Research Institute
2013-2022
Hudson Institute
2021-2022
University Hospitals of Leicester NHS Trust
2022
OhioHealth
2022
UNSW Sydney
2021
Abstract Motivation: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of vesicular nomenclatures and components, online searches for EV-related publications components currently challenging. Results: We present an improved version EVpedia, a public database EVs research. This community web portal contains identification orthologous bioinformatic tools personalized function. EVpedia includes 6879 publications, 172...
Although the importance of clathrin- and caveolin-independent endocytic pathways has recently emerged, key aspects these routes remain unknown. Using quantitative ultrastructural approaches, we show that clathrin-independent carriers (CLICs) account for approximately three times volume internalized by clathrin-mediated pathway, forming major pathway involved in uptake fluid bulk membrane fibroblasts. Electron tomographic analysis 3D morphology earliest shows they are multidomain organelles...
Polymerase I and transcript release factor (PTRF)/Cavin is a cytoplasmic protein whose expression obligatory for caveola formation. Using biochemistry fluorescence resonance energy transfer–based approaches, we now show that family of related proteins, PTRF/Cavin-1, serum deprivation response (SDR)/Cavin-2, SDR-related gene product binds to C kinase (SRBC)/Cavin-3, muscle-restricted coiled-coil (MURC)/Cavin-4, forms multiprotein complex associates with caveolae. This can constitutively...
Insulin stimulates glucose uptake into muscle and fat cells by promoting the translocation of transporter 4 (GLUT4) to cell surface. Phosphatidylinositide 3-kinase (PI3K) has been implicated in this process. However, involvement protein kinase B (PKB)/Akt, a downstream target PI3K regulation GLUT4 translocation, controversial. Here we report that microinjection PKB substrate peptide or an antibody inhibited insulin-stimulated plasma membrane 66 56%, respectively. We further examined...
Caveolar proteins and caveolae negatively regulate a second clathrin-independent endocytic CLIC/GEEC pathway; caveolin-1 affects membrane diffusion properties of raft-associated CLIC cargo, the scaffolding domain is required sufficient for inhibition.
OBJECTIVE Dysbiosis of the gut microbiota has been linked to disease pathogenesis in type 1 diabetes, yet functional consequences host this dysbiosis are unknown. We investigated interactions between and associated with diabetes risk. RESEARCH DESIGN AND METHODS performed a cross-sectional analysis stool samples from subjects recent-onset (n = 33), islet autoantibody–positive 17), low-risk autoantibody-negative 29), healthy 22). Metaproteomic was used identify gut- pancreas-derived microbial...
G9a is an epigenetic regulator that methylates H3K9, generally causing repression of gene expression, and participates in diverse cellular functions. genetically deregulated a variety tumor types can silence suppressor genes and, therefore, important for carcinogenesis. Although hypoxia recognized to be adverse factor growth metastasis, the role regulating expression has not been described extensively. Here, we show protein stability increased via reduced proline hydroxylation hence,...
The molecular mechanisms whereby caveolae exert control over cellular signaling have to date remained elusive. We therefore explored the role play in modulating Ras signaling. Lipidomic and gene array analyses revealed that caveolin-1 (CAV1) deficiency results altered lipid composition, plasma membrane (PM) phosphatidylserine distribution. These changes correlated with increased K-Ras expression extensive isoform-specific perturbation of spatial organization: CAV1-deficient cells K-RasG12V...
CAV1 (caveolin 1, caveolae protein, 22kDa) is well known as a principal scaffolding protein of caveolae, specialized plasma membrane structure. Relatively, the caveolae-independent function less studied. Autophagy process to involve various structures, including autophagosomes, lysosomes, and autolysosomes for degradation intracellular proteins organelles. Currently, in autophagy remains largely elusive. In this study, we demonstrate first time that deficiency promotes both basal inducible...
The generation of caveolae involves insertion the cholesterol-binding integral membrane protein caveolin-1 (Cav1) into membrane, however, precise molecular mechanisms are as yet unknown. We have speculated that caveolin scaffolding domain (CSD), a conserved amphipathic region implicated in interactions with signaling proteins, is crucial for caveola formation. now define core membrane-juxtaposed Cav1 and show oligomerization CSD protected by tight association both mature mammalian model...
The rapid evolution of mass spectrometry (MS)-based lipidomics has enabled the simultaneous measurement numerous lipid classes. With datasets becoming increasingly available, lipidomic-focused software tools are required to facilitate data analysis as well mining public datasets, integrating lipidomics-unique molecular information such class, chain length, and unsaturation. To address this need, we developed lipidr, an open-source R/Bioconductor package for datasets. lipidr implements a...
Telomerase enables replicative immortality in most cancers including acute myeloid leukemia (AML). Imetelstat is a first-in-class telomerase inhibitor with clinical efficacy myelofibrosis and myelodysplastic syndromes. Here, we develop an AML patient-derived xenograft resource perform integrated genomics, transcriptomics lipidomics analyses combined functional genetics to identify key mediators of imetelstat efficacy. In randomized phase II-like preclinical trial xenografts, effectively...
Hypoxia is the key pathobiological trigger of tubular oxidative stress and cell death that drives transition acute kidney injury (AKI) to chronic disease (CKD). The mitochondrial-rich proximal epithelial cells (PTEC) are uniquely sensitive hypoxia thus, pivotal in propagating sustained loss AKI-to-CKD transition. Here, we examined role PTEC-derived small extracellular vesicles (sEV) 'wave death'. Ex vivo patient-derived PTEC were cultured under normoxia (21 % O2) (1 on Transwell inserts for...
Phosphatidylinositide (PI) 3-kinase binds to tyrosyl-phosphorylated insulin receptor substrate-1 (IRS-1) in insulin-treated adipocytes, and this step plays a central role the regulated movement of glucose transporter, GLUT4, from intracellular vesicles cell surface. PDGF, which also activates PI has no significant effect on GLUT4 trafficking these cells. We propose that specificity may be mediated by differential localization response versus PDGF activation. Using subcellular fractionation...
Caveolae are an abundant feature of mammalian cells. Integral membrane proteins called caveolins drive the formation caveolae but precise mechanisms underlying caveola formation, and origin during evolution, unknown. Systematic evolutionary analysis shows conservation genes encoding in metazoans. We provide evidence for extensive ancient, local genomic gene duplication, classify distinct caveolin families. Vertebrate caveolin-1 caveolin-3 isoforms, as well invertebrate (Apis mellifera,...