A5082926542- Caveolin-1 and cellular processes
- RNA Research and Splicing
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Biotin and Related Studies
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- RNA modifications and cancer
- Signaling Pathways in Disease
- Immune Response and Inflammation
- Metabolism, Diabetes, and Cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular Mechanics and Interactions
- Lipid metabolism and disorders
- Microtubule and mitosis dynamics
- Advanced Proteomics Techniques and Applications
- Quantum-Dot Cellular Automata
- Genetic factors in colorectal cancer
- Cholesterol and Lipid Metabolism
- Biochemical and Molecular Research
- Blood disorders and treatments
- Ion channel regulation and function
- Near-Field Optical Microscopy
- Advanced Electron Microscopy Techniques and Applications
The University of Queensland
2014-2025
Research Institute for Bioscience and Biotechnology
2015
Peter MacCallum Cancer Centre
2001-2012
Swinburne University of Technology
2012
The University of Texas Southwestern Medical Center
2005-2009
The Royal Melbourne Hospital
2001
The University of Melbourne
2001
Caveolar proteins and caveolae negatively regulate a second clathrin-independent endocytic CLIC/GEEC pathway; caveolin-1 affects membrane diffusion properties of raft-associated CLIC cargo, the scaffolding domain is required sufficient for inhibition.
In mammalian cells three closely related cavin proteins cooperate with the scaffolding protein caveolin to form membrane invaginations known as caveolae. Here we have developed a novel single-molecule fluorescence approach directly observe interactions and stoichiometries in complexes from cell extracts vitro synthesized components. We show that up 50 cavins associate on caveola. However, rather than forming single coat complex containing family members, analysis reveals an exquisite...
CAV1 (caveolin 1, caveolae protein, 22kDa) is well known as a principal scaffolding protein of caveolae, specialized plasma membrane structure. Relatively, the caveolae-independent function less studied. Autophagy process to involve various structures, including autophagosomes, lysosomes, and autolysosomes for degradation intracellular proteins organelles. Currently, in autophagy remains largely elusive. In this study, we demonstrate first time that deficiency promotes both basal inducible...
Abstract Caveolae are specialized domains of the plasma membrane. Formation these invaginations is dependent on expression Caveolin-1 or -3 and proteins cavin family. In response to stress, caveolae disassemble cavins released from caveolae, allowing potentially interact with intracellular targets. Here, we describe (non-plasma membrane) interactome using biotin affinity proteomics mass spectrometry. We validate 47 potential cavin-interactor a cell-free system protein-protein binding assays....
Protein interaction networks are crucial for complex cellular processes. However, the elucidation of protein interactions occurring within highly specialised cells and tissues is challenging. Here, we describe development, application, a new method proximity-dependent biotin labelling in whole zebrafish. Using conditionally stabilised GFP-binding nanobody to target ligase GFP-labelled proteins interest, show tissue-specific proteomic profiling using existing GFP-tagged transgenic zebrafish...
Abstract Caveolae are spherically shaped nanodomains of the plasma membrane, generated by cooperative assembly caveolin and cavin proteins. Cavins cytosolic peripheral membrane proteins with negatively charged intrinsically disordered regions that flank positively α-helical regions. Here, we show three domains Cavin1 essential for caveola formation dynamic trafficking caveolae. Electrostatic interactions between promote liquid-liquid phase separation behaviour in vitro, oligomers solution,...
Caveolae are small membrane invaginations that generally stably attached to the plasma membrane. Their release is believed depend on GTPase dynamin 2 (Dyn2), in analogy with its role fission of clathrin-coated vesicles. The mechanistic understanding caveola is, however, sparse. Here, we used microscopy-based tracking individual caveolae living cells determine Dyn2 dynamics. We report associated bulb a subset caveolae, but was not required for formation or caveolae. Dyn2-positive displayed...
<title>Abstract</title> Targeted nanoparticles have the potential to revolutionize therapeutics for medical applications. Here, we demonstrate utility of a flexible precision nanovesicle delivery system functional DNA, RNA, proteins and drugs into target cells. Nanovesicles generated by membrane sculpting protein caveolin, termed caveospheres, can be loaded with or post-synthesis incorporate genetically-encoded cargo during production without need purification. Functionalized...
Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition caveolae and lipid rafts. The methods used in most these studies, however, are not able distinguish between plasma internal domains. Here we a non-detergent method for obtaining enriched derived from multiple cell types. Unexpectedly, proteins proteome suggest domains may interact with elements ER mitochondria. A comparison partial obtained other published reports identifies...
Off-resonance RF pre-saturation was used to obtain contrast in MRI images of polymer gel dosimeters irradiated doses up 50 Gy. Two different composed 2-hydroxyethyl-acrylate or methacrylic acid monomers mixed with N, N'-methylene-bisacrylamide (BIS), dispersed an aqueous gelatin matrix were evaluated. Radiation-induced polymerization the co-monomers generates a fast-relaxing insoluble polymer. Saturation using off-resonance Gaussian pulses prior spin-echo read-out short echo time leads that...
Caveolae are composed of 2 major proteins, caveolin 1 (CAV1) and cavin or polymerase transcript release factor I (CAVIN1). Here, we demonstrate that CAV1 levels modulate invasion Group A Streptococcus (GAS) into nonphagocytic mammalian cells. GAS showed enhanced internalisation CAV1-knockout mouse embryonic fibroblasts knockdown human epithelial HEp-2 cells, whereas overexpression in cells reduced invasion. This effect was not dependent on the expression fibronectin binding protein SfbI,...
Caveolae are abundant surface organelles implicated in a range of cellular processes. Two classes proteins work together to generate caveolae: integral membrane termed caveolins and cytoplasmic coat called cavins. respond stress by releasing cavins into the cytosol. A crucial aspect this model is tight regulation cytosolic pools cavin under resting conditions. We now show that recently identified region cavin1 can bind phosphoinositide (PI) lipids also major site ubiquitylation....
Background The polarized reorganization of the T cell membrane and intracellular signaling molecules in response to receptor (TCR) engagement has been implicated modulation development effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein member Scribble polarity complex, shown play role TCR signal specificity, however Dlg1 function vivo remains unclear. Methodology/Principal Findings Here we present combined data from three independently-derived dlg1-knockout mouse...
Caveolae-associated protein 3 (cavin3) is inactivated in most cancers. We characterized how cavin3 affects the cellular proteome using genome-edited cells together with label-free quantitative proteomics. These studies revealed a prominent role for DNA repair, BRCA1 and A-complex components being downregulated on deletion. Cellular cell-free expression assays direct interaction between that occurs when released from caveolae are disassembled response to UV mechanical stress. Overexpression...
The rapid spread of arthropod-borne Zika virus poses a serious public health threat that calls for effective ways controlling and treating viral infection. This in turn necessitates better understanding the mechanisms assembly its interaction with host cells. In order to facilitate such efforts, we developed new multihost expression vector pmCellFree allows multiplexed production ZIKV proteins any vitro translation system as well mammalian Using combination Leishmania cell-free AlphaLISA...
Macrophage colony stimulating factor (M-CSF or CSF-1) acts to regulate the development and function of cells macrophage lineage. Murine myeloid FDC-P1 transfected with CSF-1 receptor (FD/WT) adopt a macrophage-like morphology when cultured in CSF-1. This process is abrogated tyrosine phenyalanine substitution at position 807 (FD/807), suggesting that molecular interaction critical differentiation signaling lost (Bourette, R. P., Myles, G. M., Carlberg, K., Chen, A. R., Rohrschneider, L....
Abstract In solid tumours, elevated interstitial fluid pressure (osmotic and hydrostatic pressure) is a barrier to drug delivery correlates with poor prognosis. Glioblastoma (GBM) further experience compressive force when growing within space limited by the skull. Caveolae are proposed play mechanosensing roles, caveola‐forming proteins overexpressed in GBM. We asked whether caveolae mediate GBM response osmotic pressure. evaluated vitro influence of spontaneous or experimental...