A5015987532- Caveolin-1 and cellular processes
- Gold and Silver Nanoparticles Synthesis and Applications
- Social and Educational Sciences
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Plasmonic and Surface Plasmon Research
- Nerve injury and regeneration
- Catalytic Processes in Materials Science
- Gas Sensing Nanomaterials and Sensors
- Advanced biosensing and bioanalysis techniques
- Neurogenesis and neuroplasticity mechanisms
- Lipid metabolism and disorders
- Ion Transport and Channel Regulation
- nanoparticles nucleation surface interactions
- Neurogenetic and Muscular Disorders Research
- Cholesterol and Lipid Metabolism
- Signaling Pathways in Disease
- RNA Research and Splicing
- Electrochemical Analysis and Applications
- Digital Communication and Language
- Catalysis and Oxidation Reactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Child Development and Digital Technology
Umeå University
2015-2024
Karolinska Institutet
2023
Sahlgrenska University Hospital
2022
Chalmers University of Technology
2006-2013
Stena (Sweden)
2012-2013
Insplorion (Sweden)
2012-2013
ETH Zurich
2006
Wallenberg Wood Science Center
1999-2002
Lund University
1999
The optical responses of 75-150 nm diameter gold nanorings to changes in local refractive index have been quantified by near-infrared extinction spectroscopy and compared DDA calculations an analytical approach. "bulk" sensitivities are substantially (>5 times) larger than those nanodisks with similar diameters. Nanorings retain a significantly sensitivity at the same spectral position, demonstrating clear shape dependence that may correlate systematic difference influence dielectric...
Optical probes of heterogeneous catalytic reactions can be valuable tools for optimization and process control because they operate under realistic conditions, but often lack sensitivity. We have developed a plasmonic sensing method such based on arrays nanofabricated gold disks, covered by thin (approximately 10 nanometer) coating (catalyst support) which the catalyst nanoparticles are deposited. The particles monitor changes in surface coverage reactants during reaction through peak shifts...
Indirect nanoplasmonic sensing is a novel experimental platform for measurements of thermodynamics and kinetics in/on nanomaterials thin films. It features simple setup, high sensitivity, small sample amounts, temporal resolution (<10−3 s), operating conditions from UHV to pressure, wide temperature range, applicability any nano- or film material. The method utilizes two-dimensional arrangements Au sensor-nanoparticles coated with dielectric spacer layer onto which the material deposited....
Large area nanopatterns of functional proteins are demonstrated. A new approach to analyze atomic force microscopy height histograms is used quantify protein and antibody binding nanoscale patches. Arrays nanopatches, each containing less than 40 laminin molecules, shown be highly close 1 monoclonal anti-laminin IgG (site by IKVAV sequence) or 3−4 polyclonal IgG's per surface bound laminin. Complementary quartz crystal microbalance measurements indicate higher functionality at nanopatches on...
The conditional use of actin during clathrin-mediated endocytosis in mammalian cells suggests that the cell controls whether and how is used. Using a combination biochemical reconstitution culture, we elucidate mechanism by which coincidence PI(4,5)P2 PI(3)P curved vesicle triggers polymerization. At clathrin-coated pits, produced INPP4A hydrolysis PI(3,4)P2, this necessary for actin-driven endocytosis. Both Cdc42⋅guanosine triphosphate SNX9 activate N-WASP–WIP- Arp2/3-mediated nucleation....
Significance Caveolae are small plasma membrane invaginations that particularly abundant in adipocytes. implicated lipid uptake, but the exact mechanism is not clear. Here, we characterized physiological function of EHD2, a protein found at neck caveolae. We established link between higher caveolar mobility induced by EHD2 loss and increased cellular fatty acid uptake. Concurrently, droplets were enlarged various tissues EHD2-lacking mice. Our data suggest controls cell-autonomous,...
The levels of brain-derived neurotrophic factor (BDNF) vary between different forebrain areas and show region-specific changes after cerebral ischemia. present study explores the possibility that endogenous BDNF determine susceptibility to ischemic neuronal death. To block activity authors used TrkB-Fc fusion protein, which was infused intraventriculary in rats during 1 week before 5 or 30 minutes global Ischemic damage quantified striatum hippocampal formation reperfusion using...
Abstract Expression of mRNAs for glial cell line‐derived neurotrophic factor (GDNF), neurturin (NTN) and their receptors was studied in adult rat brain using situ hybridization after 40 kindling‐evoked, rapidly recurring seizures or 10 min global forebrain ischaemia. Following seizures, GDNF NTN were elevated dentate granule cells, c‐Ret mRNA hilar neurons non‐pyramidal cells CA1 CA3 regions. GFRα‐1 levels showed more widespread increases the layer hilus, pyramidal layers, basolateral...
Nanometer-sized hydrides are attractive for hydrogen storage. Nanofabricated model systems can be studied by a novel localized surface plasmon resonance (LSPR) based direct sensing approach and quartz crystal microbalance. The role of nanoparticle size, shape microstructure on the storage thermodynamics scrutinized. Detailed facts importance to specialist readers published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted...
In this Environmental Transmission Electron Microscopy (ETEM) study we examined the growth patterns of uniform distributions nanoparticles (NPs) using model catalysts. Pt/SiO2 was heated at 550 °C in 560 Pa O2 while Pd/carbon vacuum 500 and 300 5%H2 Argon temperatures up to 600 °C. Individual NPs Pd were tracked determine operative sintering mechanisms. We found anomalous occurred during early stages catalyst wherein some particles started grow significantly larger than mean, resulting a...
We investigate the vibrational modes of gold nanorings on a silica substrate with an ultrafast optical technique. By comparison numerical simulations, we identify several resonances in gigahertz range associated axially symmetric deformations nanoring and substrate. elucidate corresponding mode shapes find that plays important role determining damping. This study demonstrates need for plasmonic nano-optics approach to understand excitation detection mechanisms vibrations nanostructures.
Abstract Nanoplasmonic sensing has over the last two decades emerged as and diversified into a very promising experimental platform technology for studies of biomolecular interactions biomolecule detection (biosensors). Inspired by this success, in more recent years, nanoplasmonic strategies have been adapted tailored successfully probing functional nanomaterials catalysts situ real time. An increasing number these focus on using localized surface plasmon resonance (LSPR) an tool to study...
Caveolae are invaginations of the cell surface thought to regulate membrane tension, signalling, adhesion and lipid homeostasis due their dynamic behaviour ranging from stable association rounds fission fusion with plasma membrane. The caveolae coat is generated by oligomerisation protein caveolin family cavin proteins. Here, we show that cavin3 targeted cavin1 where it interacts scaffolding domain caveolin1 promote dynamics. We found N-terminal region binds a trimer N-terminus in...
The EH-domain-containing protein 2 (EHD2) is a dynamin-related ATPase that confines caveolae to the cell surface by restricting scission and subsequent endocytosis of these membrane pits. For this, EHD2 thought first bind membrane, then oligomerize, finally detach, in stringently regulated mechanistic cycle. It still unclear how ATP used this process whether binding coupled conformational changes protein. Here, we show regulatory N-terminal residues EH domain keep dimer an autoinhibited...
Caveolae are bulb-shaped invaginations of the plasma membrane (PM) that undergo scission and fusion at cell surface enriched in specific lipids. However, influence lipid composition on caveolae stability is not well described or understood. Accordingly, we inserted lipids into PM via studied their acute effects dynamics. We demonstrate sphingomyelin stabilizes to surface, whereas cholesterol glycosphingolipids drive from PM. Although all three accumulated specifically caveolae, were actively...
Lipid packing is a fundamental characteristic of bilayer membranes. It affects all membrane-associated processes ranging from curvature generation to membrane fission. Yet, we lack detailed, mechanistic understanding how lipid directly these in cellular Here, address this by focusing on caveolae, small Ω-shaped invaginations the plasma which serve as key regulators sorting and mechano-responses. In addition caveolae coat proteins, core component that critically impacts both biogenesis,...
We have developed an instrument combining localized surface plasmon resonance (LSPR) sensing with electrodeless quartz crystal microbalance dissipation monitoring (QCM-D). The two techniques can be run simultaneously, on the same sensor surface, and time resolution sensitivity as for individual techniques. QCM eliminates need to fabricate electrodes gives a large flexibility in choosing structure coating both QCM-D LSPR. performance is demonstrated liquid phase measurements of lipid bilayer...
Cellular blebbing, caused by local alterations in cell-surface tension, has been shown to increase the invasiveness of cancer cells. However, regulatory mechanisms balancing dynamics and bleb formation remain elusive. Here, we show that an acute reduction cell volume activates clathrin-independent endocytosis. Hence, a decrease surface tension is buffered internalization plasma membrane (PM) lipid bilayer. Membrane invagination endocytosis are driven tension-mediated recruitment sculpting...
Caveolae are small membrane invaginations that generally stably attached to the plasma membrane. Their release is believed depend on GTPase dynamin 2 (Dyn2), in analogy with its role fission of clathrin-coated vesicles. The mechanistic understanding caveola is, however, sparse. Here, we used microscopy-based tracking individual caveolae living cells determine Dyn2 dynamics. We report associated bulb a subset caveolae, but was not required for formation or caveolae. Dyn2-positive displayed...
Catalyst deactivation by sintering significantly reduces productivity and energy efficiency of the chemical industry effectiveness environmental cleanup processes. It also hampers introduction novel conversion devices such as fuel cells. The use experimental techniques that allow scrutiny in situ at high temperatures pressures reactive environments is a key to alleviate this situation. Today, are, however, lacking. Here, we demonstrate monitoring kinetics Pt/SiO2 model catalyst under...
Significance Eps15 (epidermal growth factor receptor pathway substrate 15)-homology domain containing proteins (EHDs) are molecular machines that use the energy of ATP binding and hydrolysis to remodel shallow membranes into highly curved membrane tubules. This activity is required in many cellular trafficking pathways. In this work, we have determined a high-resolution structure an EHD machine active state. The indicates how EHDs assemble at surface ring-like scaffolds deform underlying...