A5011247115- Electron Spin Resonance Studies
- Magnetic Properties and Synthesis of Ferrites
- Multiferroics and related materials
- ZnO doping and properties
- Cellular transport and secretion
- Glass properties and applications
- Nuclear physics research studies
- Lipid Membrane Structure and Behavior
- Luminescence Properties of Advanced Materials
- Parkinson's Disease Mechanisms and Treatments
- Magneto-Optical Properties and Applications
- Erythrocyte Function and Pathophysiology
- Transition Metal Oxide Nanomaterials
- Gas Sensing Nanomaterials and Sensors
- Lanthanide and Transition Metal Complexes
- Solid-state spectroscopy and crystallography
- Magnetic and transport properties of perovskites and related materials
- Advanced NMR Techniques and Applications
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Atomic and Molecular Physics
- Nuclear Physics and Applications
- Conducting polymers and applications
- Electromagnetic wave absorption materials
- Nuclear reactor physics and engineering
Rani Channamma University, Belagavi
2014-2025
Institute for Plasma Research
2024
Homi Bhabha National Institute
2024
University of Southern California
2004-2014
CSI Holdsworth Memorial Hospital
2013
PES University
2013
University of California, Los Angeles
2005
University of California, Irvine
2005
Jain University
1997-2000
alpha-Synuclein is known to play a causative role in Parkinson disease. Although its physiological functions are not fully understood, alpha-synuclein has been shown interact with synaptic vesicles and modulate neurotransmitter release. However, the structure of physiologically relevant membrane-bound state remains unknown. Here we developed site-directed spin labeling EPR-based approach for determining bound lipid bilayer. Continuous-wave EPR was used assign local secondary determine...
Partially folded proteins, characterized as exhibiting secondary structure elements with loose or absent tertiary contacts, represent important intermediates in both physiological protein folding and pathological misfolding. To aid the characterization of structural state(s) such a novel calculation scheme is presented that combines restraints derived from pulsed EPR NMR spectroscopy. The methodology established for α-synuclein (αS), which exhibits characteristics partially when bound to...
Misfolding and amyloid fibril formation by human islet polypeptide (hIAPP) are thought to be important in the pathogenesis of type 2 diabetes, but structures misfolded forms remain poorly understood. Here we developed an approach that combines site-directed spin labeling with continuous wave pulsed EPR investigate local secondary structure determine relative orientation elements respect each other. These data indicated individual hIAPP molecules take up a hairpin fold within fibril. This...
α-Synuclein (αS) is a protein with multiple conformations and interactions. Natively unfolded in solution, αS accumulates as amyloid neurological tissue Parkinson disease interacts membranes under both physiological pathological conditions. Here, we used cryoelectron microscopy conjunction electron paramagnetic resonance (EPR) other techniques to characterize the ability of remodel vesicles. At molar ratios 1:5 1:40 for protein/lipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol), large...
Significance BIN/amphiphysin/Rvs (BAR) proteins shape lipid membranes into functional shapes and compartments in a broad range of cellular processes. However, how protein generates distinct forms membrane curvature these states are regulated is unknown. We find that endophilin A1 uses different structures mechanisms to generate tubes or vesicles. Phosphorylation toggles conformational switch between two structures, potentially acting as regulator vivo. These findings may also explain other...
The most common chromosomal translocation in cancer, t(14;18), occurs at the bcl-2 major breakpoint region (Mbr) follicular lymphomas. 150-bp Mbr, which contains three breakage hotspots (peaks), has a single-stranded character and, hence, non-B DNA conformation both vivo and vitro. Here, we use gel assays electron microscopy to show that triplex-specific antibody binds Mbr Bisulfite reactivity shows structure is favored by, but not dependent upon, supercoiling suggests possible triplex one...
Control of membrane curvature is required in many important cellular processes, including endocytosis and vesicular trafficking. Endophilin a bin/amphiphysin/rvs (BAR) domain protein that induces vesicle formation by promotion through binding as dimer. Using site-directed spin labeling EPR spectroscopy, we show the overall BAR structure rat endophilin A1 dimer determined crystallographically maintained under predominantly vesiculating conditions. Spin-labeled side chains on concave surface...
Abstract Electron paramagnetic resonance using site‐directed spin labeling can be used as an approach for determination of protein structures that are difficult to solve by other methods. One important aspect this is the measurement interlabel distances double electron–electron (DEER) method. Interpretation experimental data could facilitated a computational calculation distances. We describe algorithm, PRONOX, rapid computation based on label conformer distributions at any site protein. The...
Significance Eps15 (epidermal growth factor receptor pathway substrate 15)-homology domain containing proteins (EHDs) are molecular machines that use the energy of ATP binding and hydrolysis to remodel shallow membranes into highly curved membrane tubules. This activity is required in many cellular trafficking pathways. In this work, we have determined a high-resolution structure an EHD machine active state. The indicates how EHDs assemble at surface ring-like scaffolds deform underlying...
Amelogenin is a proline-rich enamel matrix protein known to play an important role in the oriented growth of crystals. self-assembles form nanospheres and higher order structures mediated by hydrophobic interactions. This study aims obtain better insight into relationship between primary-secondary structure self-assembly amelogenin applying computational biophysical methods. Variable temperature circular dichroism studies indicated that under physiological pH recombinant full-length porcine...
We characterized the structure of partially unfolded bacteriorhodopsin in sodium dodecyl sulfate (SDS) micelles and compared it with its vitro refolded after reconstitution dimyristoylphosphatidylcholine/3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (DMPC/CHAPS). Intrahelical interhelical distances were mapped protein using strategically located spin-label pairs at helical ends, assayed by pulsed electron paramagnetic resonance spectroscopy (double electron-electron spin...
The complete and incomplete fusion cross sections for the $^{7}$Li+$^{205}$Tl reaction were measured at near barrier energies by online characteristic $\gamma$ ray detection technique. (CF) above Coulomb found to be suppressed $\sim$ 26 \% compared coupled channel calculations. Reduced present system normalised also systematically lower than those with strongly bound projectiles forming a similar compound nucleus. suppression observed in CF is commensurate total (ICF) sections. In ICF...
The complete fusion (CF), incomplete (ICF), and neutron transfer (\(1n\) stripping, \(2n\) \(1n\) pickup) cross sections for the \(^{7}\mathrm {Li}+{^{205}\mathrm {Tl}}\) system were measured at energies around Coulomb barrier by online \(\gamma \)‑ray detection technique. CF found to be suppressed above-barrier compared one-dimensional penetration model (1DBPM) as well coupled channel (CC) calculations. However, below-barrier are enhanced 1DBPM in reasonable agreement with CC suppression...