A5047738067- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Liver Disease Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Drug Transport and Resistance Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Erythrocyte Function and Pathophysiology
- Cancer, Stress, Anesthesia, and Immune Response
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Endometriosis Research and Treatment
- Escherichia coli research studies
- Cancer Mechanisms and Therapy
- Cancer Immunotherapy and Biomarkers
- Force Microscopy Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
The University of Texas Southwestern Medical Center
2015-2025
Southwestern Medical Center
2013-2024
University of Gothenburg
2015
Institute for Biomedicine
2015
Scripps Research Institute
2008-2013
UCLouvain
2002-2008
de Duve Institute
2004-2007
Total internal reflection fluorescence microscopy (TIR-FM) has become a powerful tool for studying clathrin-mediated endocytosis. However, due to difficulties in tracking and quantifying their heterogeneous dynamic behavior, detailed analyses have been restricted limited number of selected clathrin-coated pits (CCPs). To identify intermediates the formation vesicles factors that regulate progression through these stages, we used particle-tracking software statistical methods establish an...
Clathrin-mediated endocytosis of surface receptors and their bound ligands (i.e., cargo) is highly regulated, including by the cargo itself. One possible sources observed heterogeneous dynamics clathrin-coated pits (CCPs) might be different content. Consistent with this, we show that CCP size dynamic behavior varies low density lipoprotein receptor (LDLR) expression levels in a manner dependent on LDLR-specific adaptors, Dab2 ARH. In Dab2-mCherry–expressing cells, varying LDLR leads to...
Biomolecular condensates participate in the regulation of gene transcription, yet relationship between nuclear condensation and transcriptional activation remains elusive. Here, we devised a biotinylated CRISPR-dCas9-based optogenetic method, light-activated macromolecular phase separation (LAMPS), to enable inducible formation, affinity purification, multiomic dissection at targeted genomic loci. LAMPS-induced enhancers promoters activates endogenous transcription by chromatin...
Severe COVID-19 is characterized by an increase in the number and changes function of innate immune cells including neutrophils. However, it not known how metabolome patients with COVID-19. To address these questions, we analyzed neutrophils from severe or mild healthy controls. We identified widespread dysregulation neutrophil metabolism disease progression amino acid, redox, central carbon metabolism. Metabolic were consistent reduced activity glycolytic enzyme GAPDH. Inhibition GAPDH...
Diverse cargo molecules (i.e., receptors and ligand/receptor complexes) are taken into the cell by clathrin-mediated endocytosis (CME) utilizing a core machinery consisting of cargo-specific adaptors, clathrin GTPase dynamin. Numerous endocytic accessory proteins also required, but their differential roles functional hierarchy during CME not yet understood. Here, we used combination quantitative live-cell imaging total internal reflection fluorescence microscopy (TIR-FM), decomposition...
Cholera toxin (CT) enters and intoxicates host cells after binding cell surface receptors using its B subunit (CTB). The ganglioside (glycolipid) GM1 is thought to be the sole CT receptor; however, mechanism by which CTB mediates internalization of remains enigmatic. Here we report that binds glycoproteins. Relative contributions gangliosides glycoproteins depend on type, primarily in colonic epithelial lines. Using a metabolically incorporated photocrosslinking sugar, identified one...
The conditional use of actin during clathrin-mediated endocytosis in mammalian cells suggests that the cell controls whether and how is used. Using a combination biochemical reconstitution culture, we elucidate mechanism by which coincidence PI(4,5)P2 PI(3)P curved vesicle triggers polymerization. At clathrin-coated pits, produced INPP4A hydrolysis PI(3,4)P2, this necessary for actin-driven endocytosis. Both Cdc42⋅guanosine triphosphate SNX9 activate N-WASP–WIP- Arp2/3-mediated nucleation....
A well-orchestrated hierarchy of molecular events is required for successful initiation and maturation clathrin-coated pits (CCPs). Nevertheless, CCPs display a broad range lifetimes. This dynamic heterogeneity could either reflect differences in the temporal events, or similar CCP processes with variable kinetics. To address this question, we have used multi-channel image acquisition automated analysis dynamics combination new method to quantify time courses recruitment endocytic factors...
Ikarugamycin ( IKA ) is a previously discovered antibiotic, which has been shown to inhibit the uptake of oxidized low‐density lipoproteins in macrophages. Furthermore, several groups have used clathrin‐mediated endocytosis CME plant cell lines. However, detailed characterization yet be performed. Consequently, we performed biochemistry and microscopy experiments further characterize effects on . We show that an IC 50 2.7 μ m H1299 cells acutely inhibits , but not other endocytic pathways,...
Highlights•CLCb and Dyn1 contribute to adaptive clathrin-mediated endocytosis in cancer cells•CLCb modulates dynamin-1 via an APPL1/Akt/GSK3β signaling circuit•Upregulation of CLCb alters EGFR trafficking dependent on dynamin-1•Upregulation increases tumor cell migration metastasisSummarySignaling receptors are internalized regulated by (CME). Two clathrin light chain isoforms, CLCa CLCb, integral components the endocytic machinery whose differential functions remain unknown. We report that...
We addressed the role of Src on cortical actin dynamics and polarized endocytosis in MDCK cells harboring a thermosensitive v‐src mutant. Shifting monolayers established at 40 °C (non‐permissive temperature) to 34 (permissive rapidly reactivated v‐Src kinase, but tight junctions cell polarity resisted for >6 h. At this interval, activated was recruited apical vesicles, induced cortactin‐associated circular ruffles productive macropinosomes, thereby accelerating pinocytosis by...
<i>Background:</i> In vitro studies suggest that the transcription factor nuclear factor-kappa B (NF-ĸB) is implicated in transduction of proinflammatory signals endometriosis. The aim this study was to investigate involvement NF-ĸB and processes regulated by initial development endometriotic lesionsin vivo<i>.</i><i>Methods:</i> Endometriosis induced nude mice intraperitoneal injection fluorescent-labeled menstrual endometrium. Two inhibitors (BAY 11-7085...
The formation of clathrin-coated pits (CCPs) at the plasma membrane has been reported to sometimes occur repeatedly predefined sites. However, defining such CCP 'hotspots' structurally and mechanistically difficult due dynamic heterogeneous nature CCPs. Here, we explore molecular requirements for hotspots using a global assay dynamics. Our data confirmed that subset CCPs is nucleated spatially distinct degree clustering nucleation events these sites dependent on integrity cortical actin,...