A5011666786- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Genomics and Phylogenetic Studies
- Data Analysis with R
- Environmental DNA in Biodiversity Studies
- Mitochondrial Function and Pathology
- Protein Degradation and Inhibitors
- Cellular Mechanics and Interactions
- Liver physiology and pathology
- Species Distribution and Climate Change
- Microbial Community Ecology and Physiology
- Pancreatic function and diabetes
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
The University of Texas Southwestern Medical Center
2023-2025
Johns Hopkins Medicine
2022-2025
Johns Hopkins University
2022-2025
Southwestern Medical Center
2024
Abstract The efficacy of current antimitotic cancer drugs is limited by toxicity in highly proliferative healthy tissues. A cancer-specific dependency on the microtubule motor protein KIF18A therefore makes it an attractive therapeutic target. Not all cancers require KIF18A, however, and determinants underlying this distinction remain unclear. Here, we show that inhibition drives a modest widespread increase spindle assembly checkpoint (SAC) signaling from kinetochores which can result...
Hepatocyte polyploidization is a tightly controlled process that initiated at weaning and increases with age. The proliferation of polyploid hepatocytes in vivo restricted by the PIDDosome-P53 axis, but how this pathway triggered remains unclear. Given increased hepatocyte ploidy protects against malignant transformation, evolutionary driver sets upper limit for unknown. Here we show accumulate centrioles during cycles vivo. presence excess mature containing ANKRD26 was required to activate...
Studying essential genes required for dynamic processes in live mice is challenging as genetic perturbations are irreversible and limited by slow protein depletion kinetics. The auxin-inducible degron (AID) system a powerful tool analyzing inducible loss vitro, but it toxic to mice. Here, we use an optimized second-generation AID achieve the conditional reversible of centrosomal CEP192 We show that auxin derivative 5-phenyl-indole-3-acetic acid well tolerated over 2 weeks drives...
Abstract Studying essential genes required for dynamic processes in live mice is challenging as genetic perturbations are irreversible and limited by slow protein depletion kinetics. The first-generation auxin-inducible-degron (AID) system a powerful tool analyzing inducible loss cultured cells. However, auxin administration toxic to mice, preventing its long-term use animals. Here, we an optimized second-generation AID achieve the conditional reversible of centrosomal CEP192 mice. We show...
Local metabolic demand within cells varies widely and the extent to which individual mitochondria can be specialized meet these functional needs is unclear. We examined subcellular distribution of MICOS, a spatial organizer mitochondria, discovered that it dynamically enriches at tip minor population in cell periphery we term "METEORs". METEORs have unique composition; MICOS enrichment sites are depleted mtDNA matrix proteins contain high levels Ca
Knowledge of which organisms have publicly available reference sequences at known DNA barcoding loci is crucial for both metabarcoding studies and the design new primers. In a study, taxa interest can not be detected if there are no labeled to compare to. Similarly, designing species-specific primers only possible enough target their phylogenetic relatives represent genetic diversity across individuals. The purpose this tool allow scientists automatically gather from NCBI, BOLD CRUX. It...
Knowledge of which organisms have publicly available reference sequences at known DNA barcoding loci is crucial for both metabarcoding studies and the design new primers. In a study, taxa interest can not be detected if there are no labeled to compare to. Similarly, designing species-specific primers only possible enough target their phylogenetic relatives represent genetic diversity across individuals. The purpose this tool allow scientists automatically gather from NCBI, BOLD CRUX. It...
Land managers, researchers, and regulators increasingly utilize environmental DNA (eDNA) techniques to monitor species richness, presence, absence. In order properly develop a biological assay for eDNA metabarcoding or quantitative PCR, scientists must be able find not only reference sequences (previously identified in genomics database) that match their target taxa but also non-target taxa. Determining which have publicly available time-efficient accurate manner currently requires...
Abstract Land managers, researchers, and regulators increasingly utilize environmental DNA (eDNA) techniques to monitor species richness, presence, absence. In order properly develop a biological assay for eDNA metabarcoding or quantitative PCR, scientists must be able find not only reference sequences (previously identified in genomics database) that match their target taxa but also non-target taxa. Determining which have publicly available time-efficient accurate manner currently requires...