A5023844310- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- ATP Synthase and ATPases Research
- Optical Coherence Tomography Applications
- Metabolism and Genetic Disorders
- Parasites and Host Interactions
- Image Processing Techniques and Applications
- Parasite Biology and Host Interactions
- Microtubule and mitosis dynamics
- Helminth infection and control
- Nuclear Structure and Function
- Cellular Mechanics and Interactions
- Immune responses and vaccinations
The University of Texas Southwestern Medical Center
2022-2025
Southwestern Medical Center
2023-2024
Children's Medical Center
2023-2024
Abstract Helminth infections are common in animals. However, the impact of a helminth infection on function hematopoietic stem cells (HSCs) and other has not been comprehensively defined. In this article, we describe response to mice with Schistosoma mansoni, parasitic flatworm that causes schistosomiasis. We analyzed frequency or number cell types bone marrow, spleen, liver, thymus, blood observed multiple changes caused by infection. Schistosome impaired marrow HSC after serial...
Abstract Mitochondria are highly dynamic double membrane-bound organelles that exist in a semi- continuous network. Mitochondrial morphology arises from the complex interplay of numerous processes, including opposing fission and fusion dynamics formation organized cristae invaginations inner membrane. While extensive work has examined mechanisms mitochondrial fission, it remains unclear how is coordinated across two membrane bilayers organization coupled with dynamics. Previously, yeast...
While extensive work has examined the mechanisms of mitochondrial fission, it remains unclear whether internal proteins in metazoans play a direct role process. Previously, yeast inner membrane protein Mdm33 was shown to be required for normal morphology and been hypothesized involved fission. However, is unknown plays role, not thought have mammalian homolog. Here, we use bioinformatic approach identify structural ortholog humans, CCDC51 (also called MITOK), whose depletion phenocopies loss...
Abstract Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence microscope without requiring high laser powers or specialized fluorophores. However, excitation out-of-focus can accelerate photobleaching and phototoxicity. In contrast, light-sheet (LSFM) largely avoids exciting fluorescence, thereby enabling volumetric imaging with low photo-bleaching intrinsic optical sectioning. Combining SIM LSFM would enable gentle 3D at doubled resolution. multiple...
Mitochondria perform a variety of critical cellular functions, including producing energy via oxidative phosphorylation, hosting number anabolic reactions, and maintaining calcium homeostasis. Cells have distinct subcellular local metabolic needs, which begs the question whether different populations mitochondria are specialized to play unique roles. Mitochondrial form function intricately linked, in order these diverse organized into functional morphological domains by Contact Site Cristae...
Local metabolic demand within cells varies widely and the extent to which individual mitochondria can be specialized meet these functional needs is unclear. We examined subcellular distribution of MICOS, a spatial organizer mitochondria, discovered that it dynamically enriches at tip minor population in cell periphery we term "METEORs". METEORs have unique composition; MICOS enrichment sites are depleted mtDNA matrix proteins contain high levels Ca
Helminth infections are common in animals. However, the impact of a helminth infection on function hematopoietic stem cells (HSCs) and other has not been comprehensively defined. Here we describe response to mice with