A5030516594- Cancer Mechanisms and Therapy
- Peptidase Inhibition and Analysis
- Phagocytosis and Immune Regulation
- MXene and MAX Phase Materials
- Ion Channels and Receptors
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Multiple Myeloma Research and Treatments
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Calcium signaling and nucleotide metabolism
- Signaling Pathways in Disease
- Neurobiology and Insect Physiology Research
- Protein Degradation and Inhibitors
- Plant Stress Responses and Tolerance
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Endoplasmic Reticulum Stress and Disease
- Hemoglobinopathies and Related Disorders
- Immune Response and Inflammation
- Liver Disease and Transplantation
- Heat shock proteins research
Moffitt Cancer Center
2019-2024
La Jolla Institute for Immunology
2012-2021
Saarland University
2004-2016
Harvard University Press
2013
Harvard University
2012
T helper (Th) cell activation is required for the adaptive immune response. Formation of immunological synapse (IS) between Th cells and antigen-presenting essential activation. IS formation induces polarization redistribution many signaling molecules; however, very little known about organelle during in cells. We show that induced cytoskeleton-dependent mitochondrial to immediate vicinity IS. Using total internal reflection microscopy, we found upon stimulation, distance mitochondria was...
A rise of the intracellular Ca(2+) concentration has multiple signaling functions. Sustained influx across plasma membrane through calcium release-activated (CRAC) channels is required for T-cell development in thymus, gene transcription, and proliferation differentiation naïve T-cells into armed effectors cells. Intracellular signals are shaped by mitochondria, which function as a highly dynamic buffer. However, precise role mitochondria Ca(2+)-dependent activation unknown. Here we have...
Formation of an immunological synapse (IS) between APC and T cells activates calcium entry through ORAI channels, which is indispensable for cell activation. Successful proliferation maturation naive possible only if premature inactivation channels prevented. Although it undisputed that required function, not known influx uniformly distributed over the plasma membrane or preferential local sites (for instance, at IS) exist. In this study, we show mitochondrial positioning determines...
Significance Close appositions between the endoplasmic reticulum (ER) and plasma membrane in mammalian cells have essential roles cellular lipid metabolism cytoplasmic calcium signaling. Although recent investigations yielded considerable insight into structural basis for transfer at ER–plasma junctions, little is known about proteins that organize junctions Our data show ER protein transmembrane 110 (TMEM110) supports maintenance of competent signaling acts concert with other dynamic...
Educational psychology plays a fundamental role in professional development and language stimulation, especially the Cuban context. This article explores how principles of this discipline can be applied to improve these aspects, considering theories contemporary educators. Professional is vital for teacher growth, as it allows updating knowledge methodologies through meaningful learning that connects with educators' prior experiences. In constantly changing world marked by economic,...
Abstract ORAI1 Ca 2+ channels in the plasma membrane (PM) are gated by STIM1 at endoplasmic reticulum (ER)-PM junctions to effect store-dependent entry into cells, but little is known about how local STIM-ORAI signalling coordinated with overall cellular architecture. Filamentous septins can specify cytoskeletal rearrangements and have been found recently modulate signalling. Here we show super-resolution imaging of ORAI1, STIM1, septin 4 living cells that facilitate indirectly. Septin does...
Abstract Clonal T cell expansion through proliferation is a central process of the adaptive immune response. Apoptosis activated cells required to avoid chronic inflammation. and apoptosis are often analyzed with stimuli that do not induce formation functional immunological synapse. Here we analyze Ca 2+ dependence in primary human CD4 + following stimulation anti‐CD3/anti‐CD28‐coated beads, which tight interaction similar We found this focal be much more efficient for stimulating IL‐2...
Abnormal vascular smooth muscle cell (VSMC) proliferation contributes to occlusive and proliferative disorders of the vessel wall. Salicylate other nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit VSMC by an unknown mechanism unrelated activity. In search for this mechanism, we have studied effects salicylate NSAIDs on subcellular Ca(2+) homeostasis Ca(2+)-dependent in rat aortic A10 cells, a model neointimal VSMCs. We found that cells displayed both store-operated entry (SOCE)...
Abstract Viral hepatitis ranks as the fifth cause of morbidity for infectious diseases in Cuba. Epidemics are observed frequently population, A virus being main agent responsible such epidemics. Previous reports also confirmed circulation E virus. From 1998 to 2003, 258 serum samples were collected by Reference Laboratory on Hepatitis during 33 outbreaks acute viral well from 39 sporadic clinical cases. Sera tested anti‐HAV and anti‐HEV IgM EIA. Overall studied sera 12 (36.4%) positive only,...
Lymphocyte signaling and activation leads to the influx of extracellular Ca 2+ via release activated (CRAC) channels in plasma membrane. Activation CRAC occurs following emptying endoplasmic reticulum intracellular stores. One model explain coupling store‐emptying is secretion‐like conformational model. This proposes that store depletion increases junctions between membrane a manner could be regulated by cortical actin cytoskeleton. Here, we show stabilization or depolymerization...
A seroepidemiological study of hepatitis E virus (HEV) infection was conducted in a district Havana, where (HAV) is considered endemic. The levels anti-HEV antibodies were evaluated by enzyme-linked immunosorbent assay (ELISA) based on the recombinant protein GST-ORF2.1. Anti-HEV detected 11 209 (5.3%) serum samples, compared to 71.3% for anti-HAV antibodies. No risk factors reported previously HEV showed significant association with presence antibodies, whereas strongly associated...
Abstract Activation of the adaptive immune response requires interaction between antigen-presenting cells and T-cells. This cell-cell interaction, called immunological synapse (IS), facilitates activation several T-cell receptor (TCR)-mediated signalling cascades including a rise in cytosolic Ca2+ concentration ([Ca2+]i) through CRAC/ORAI1 channels. These channels are opened after depletion intracellular stores inactivated by inflowing itself. We show epifluorescence, 2-photon total internal...