A5013957802- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Cell death mechanisms and regulation
- Diet and metabolism studies
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Cardiac Ischemia and Reperfusion
- Biochemical Acid Research Studies
- Neuroscience and Neuropharmacology Research
- Peroxisome Proliferator-Activated Receptors
- RNA modifications and cancer
- Sirtuins and Resveratrol in Medicine
- Peptidase Inhibition and Analysis
- PI3K/AKT/mTOR signaling in cancer
- Enzyme Catalysis and Immobilization
- Chemical Synthesis and Analysis
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Biochemical effects in animals
University of California, San Diego
2015-2024
Cytokinetics (United States)
2019-2024
Metropolitan State University of Denver
2024
Murphy Oil Corporation (United States)
2011-2023
University of California San Diego Medical Center
2022
Poseida Therapeutics (United States)
2022
McGill University Health Centre
2022
Johns Hopkins University
2021
TRIUMF
2020
La Jolla Alcohol Research
2011-2019
Several lines of evidence indicate that mitochondrial reactive oxygen species (ROS) generation is the major source oxidative stress in cell. It has been shown ROS production accompanies cytochrome c release different apoptotic paradigms, but site(s) remain obscure. In current study, we demonstrate loss by mitochondria oxidizing NAD(+)-linked substrates results a dramatic increase and respiratory inhibition. This increased can be mimicked rotenone, complex I inhibitor, as well other chemical...
Recently developed technologies have enabled multi-well measurement of O(2) consumption, facilitating the rate mitochondrial research, particularly regarding mechanism action drugs and proteins that modulate metabolism. Among these technologies, Seahorse XF24 Analyzer was designed for use with intact cells attached in a monolayer to tissue culture plate. In order high throughput assay system which both energy demand substrate availability can be tightly controlled, we protocol expand...
Metabolic reprogramming is emerging as a hallmark of the innate immune response, and dynamic control metabolites such succinate serves to facilitate execution inflammatory responses in macrophages other cells. Immunoresponsive gene 1 (Irg1) expression induced by stimuli, its enzyme product cis-aconitate decarboxylase catalyzes production itaconate from tricarboxylic acid cycle. Here we identify an immunometabolic regulatory pathway that links Irg1 accumulation occurs context responses....
Diabetic microvascular complications have been considered to be mediated by a glucose-driven increase in mitochondrial superoxide anion production. Here, we report that production was reduced the kidneys of steptozotocin-induced mouse model type 1 diabetes, as assessed vivo real-time transcutaneous fluorescence, confocal microscopy, and electron paramagnetic resonance analysis. Reduction biogenesis phosphorylation pyruvate dehydrogenase (PDH) were observed from diabetic mice. These...
Expression of the human protooncogene bcl-2 protects neural cells from death induced by many forms stress, including conditions that greatly elevate intracellular Ca2+. Considering Bcl-2 is partially localized to mitochondrial membranes and excessive Ca2+ uptake can impair electron transport oxidative phosphorylation, present study tested hypothesis mitochondria Bcl-2-expressing have a higher capacity for energy-dependent greater resistance Ca(2+)-induced respiratory injury than do not...
The mitochondrial inner membrane (IM) serves as the site for ATP production by hosting oxidative phosphorylation complex machinery most notably on crista membranes. Disruption of structure has been implicated in a variety cardiovascular and neurodegenerative diseases. Here, we characterize ChChd3, previously identified PKA substrate unknown function (Schauble, S., King, C. C., Darshi, M., Koller, A., Shah, K., Taylor, S. (2007) J. Biol. Chem. 282, 14952-14959), show that it is essential...
Facilitated pyruvate transport across the mitochondrial inner membrane is a critical step in carbohydrate, amino acid, and lipid metabolism. We report that clinically relevant concentrations of thiazolidinediones (TZDs), widely used class insulin sensitizers, acutely specifically inhibit carrier (MPC) activity variety cell types. Respiratory inhibition was overcome with methyl pyruvate, localizing effect to facilitated transport, knockdown either paralog, MPC1 or MPC2, decreased EC 50 for...
Abstract Oncogenic mutations in isocitrate dehydrogenase 1 and 2 (IDH1/2) occur several types of cancer, but the metabolic consequences these genetic changes are not fully understood. In this study, we performed 13C flux analysis on a panel isogenic cell lines containing heterozygous IDH1/2 mutations. We observed that under hypoxic conditions, IDH1-mutant cells exhibited increased oxidative tricarboxylic acid metabolism along with decreased reductive glutamine metabolism, IDH2-mutant cells....
Up-regulation of glucose metabolism has been implicated not only in tumor cell growth but also immune cells upon activation. However, little is known about the metabolite profile rheumatoid arthritis (RA), particularly fibroblast-like synoviocytes (FLS). This study was undertaken to evaluate whether changes RA FLS could play a role inflammation and joint damage.Synovium were obtained from patients with osteoarthritis (OA). The rate glycolysis after stimulation lipopolysaccharide...
Resource20 May 2020Open Access Source DataTransparent process A novel approach to measure mitochondrial respiration in frozen biological samples Rebeca Acin-Perez Corresponding Author [email protected] orcid.org/0000-0001-9553-8337 Department of Medicine, Endocrinology, David Geffen School University California, Los Angeles, CA, USA Metabolism Theme, Search for more papers by this author Ilan Y Benador Nutrition and Metabolism, Graduate Medical Sciences, Boston Boston, MA, Anton Petcherski...
Tissue inhibitor of metalloproteinase-2 (TIMP-2), a protease that binds to the latent and active forms 72 kDa type IV collagenase (gelatinase A), was found inhibit in vitro proliferation human microvascular endothelial (HME) cells stimulated with bFGF 5% serum. The maximal inhibitory effect TIMP-2 on incorporation 3H-thymidine evident 24 hours after stimulation these ranged between 45 60%. half-maximal effective concentration 107 +/- 12 nM (S.D.). In contrast, TIMP-1 not slow growth HME...