A5028171031- Osteoarthritis Treatment and Mechanisms
- Estrogen and related hormone effects
- Bone and Joint Diseases
- Bone Metabolism and Diseases
- Rheumatoid Arthritis Research and Therapies
- Orthopedic Infections and Treatments
- Reproductive System and Pregnancy
- Cytokine Signaling Pathways and Interactions
- Nanoparticle-Based Drug Delivery
- Inflammatory mediators and NSAID effects
- Hormonal and reproductive studies
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Advanced Polymer Synthesis and Characterization
- Antimicrobial Peptides and Activities
- Retinoids in leukemia and cellular processes
- Psoriasis: Treatment and Pathogenesis
- Bone health and osteoporosis research
- Advanced Drug Delivery Systems
- Knee injuries and reconstruction techniques
- Hydrogels: synthesis, properties, applications
- Microbial Natural Products and Biosynthesis
- Tryptophan and brain disorders
Chalmers University of Technology
2021-2025
University of Gothenburg
2011-2022
University of California, San Diego
2017-2020
University of Montana
2017-2018
Sahlgrenska University Hospital
2018
Centre for Inflammation Research
2015
Stimuli-responsive nanoparticles (NPs) are especially interesting to enhance the drug delivery specificity for biomedical applications. With aim achieve a highly stable and inflammation-specific release, we designed reactive oxygen species (ROS)-responsive dextran–drug conjugate (Nap–Dex). By blending Nap–Dex with acid-sensitive acetalated dextran polymer, achieved dual-responsive NP high toward inflammatory environment. The environment not only has elevated ROS levels but also lower pH than...
The bone-sparing effect of estrogen is primarily mediated via receptor-α (ERα), which stimulates target gene transcription through two activation functions (AFs), AF-1 in the N-terminal and AF-2 ligand binding domain. To evaluate role ERα for effects bone vivo, we analyzed mouse models lacking entire protein (ERα −/− ), (ERαAF-1 0 or (ERαAF-2 ). Estradiol (E2) treatment increased amount both trabecular cortical ovariectomized (OVX) WT mice. Neither nor responded to E2 OVX ERαAF-2 ERαAF-1...
Abstract Testosterone deficiency in men is associated with increased risk for autoimmunity and B cell numbers through unknown mechanisms. Here we show that testosterone regulates the cytokine BAFF, an essential survival factor cells. Male mice lacking androgen receptor have splenic numbers, serum BAFF levels Baff mRNA. by castration causes expansion of BAFF-producing fibroblastic reticular cells (FRCs) spleen, which may be coupled to lower noradrenaline castrated males, as α-adrenergic...
Significance Estrogen exerts important effects in the skeleton, which are primarily mediated via estrogen receptor (ER)α, stimulates target gene transcription through two activation functions (AFs), AF-1 N-terminal and AF-2 ligand-binding domain. Previous studies demonstrate that ERα ligands might act as agonists, partial or antagonists. We antagonist ICI 182,780 (ICI) acts a tissue-dependent manner mice lacking ERαAF-2, resulting no effect, agonistic activity, inverse activity. Importantly,...
Osteoarthritis (OA) is characterized by chronic, low-grade inflammation that contributes to cartilage degradation and joint pain. We previously identified Siglecs in the synovial fluid of OA patients (OA-SF), together with studies implicating arthritic diseases prompted us investigate interplay between Siglec-5, Siglec-9, TLR4 monocyte regulation during OA. Flow cytometric profiling revealed an inverse correlation Siglec-5 expression activity, but not suggesting engagement may suppress...
A more vigorous immune system activation is generally seen in women as compared to men. The reasons for these differences are still not understood. By investigating the immune-regulatory role of estrogens, we have previously shown that estradiol (E2) can regulate and ameliorate rheumatoid arthritis models. aim this study was elucidate ovariectomy (ovx) innate responses.Female mice were ovx or sham operated. After three weeks, either dorsal air pouches established by injections sterile with...
Lasofoxifene (las) and bazedoxifene (bza) are third generation selective estrogen receptor modulators (SERMs) with minimal estrogenic side effects, approved for treatment of postmenopausal osteoporosis. T cells involved in the pathology osteoporosis previous studies have established an important role 17β-estradiol (E2) cell development function. E2 causes a drastic thymic atrophy, alters composition populations, inhibits dependent inflammation. In contrast, second SERM raloxifene (ral) lacks...
Estrogen (E2) delays onset and decreases severity of experimental arthritis. The aim this study was to investigate the importance total estrogen receptor alpha (ERα) expression cartilage-specific ERα in genetically modified mice for ameliorating effect treatment Mice with (total ERα-/-) or (Col2α1-ERα-/-) inactivation wild-type (WT) littermates were ovariectomized, treated E2 placebo, induced antigen-induced arthritis (AIA). At termination, knees collected histology, synovial splenic cells...
Upconverting nanoparticles (UCNPs) are potentially useful for biological applications, if they capable of high-intensity emission. This requires the highest absorption efficiencies wavelengths not absorbed or scattered by tissues. 800 nm is considered to be a "biobenign" wavelength because it effectively minimizes signal attenuation and reduces detrimental overheating, while maintaining deep tissue penetration. Neodymium (Nd3+) substitution ytterbium (Yb3+) in lanthanide-based UCNPs...
Drug delivery strategies for joint diseases need to overcome the negatively charged cartilage matrix. Previous studies have extensively investigated particle approaches increase uptake efficiency by harnessing anionic charge of but neglected address potential interactions with protein-rich biological environment space. We aimed evaluate effects hard protein coronas derived from osteoarthritis (OA) and rheumatoid arthritis (RA) patient synovial fluids as well commonly used fetal calf serum...
Developing photoactivatable theranostic platforms with integrated functionalities of biocompatibility, targeting, imaging contrast, and therapy is a promising approach for cancer diagnosis therapy. Here, we report agent based on hybrid nanoparticle comprising fullerene nanocrystals gold nanoparticles (FGNPs) photoacoustic photothermal Compared to crystals, FGNPs exhibited stronger signals heating characteristics by irradiating light an optimal wavelength. Our studies demonstrated that could...
Objective Bone loss in arthritis is a complex process characterized by bone erosions and periarticular generalized loss. The antigen‐induced (AIA) model mainly used to study synovitis joint destruction, including erosions; however, has been less extensively investigated. objectives of this were characterize establish AIA as for loss, determine the importance NADPH oxidase 2 (NOX‐2)–derived reactive oxygen species (ROS) Methods Arthritis was induced mice local injection antigen one knee;...
Objective. RA predominantly affects post-menopausal women and is strongly associated with development of generalised osteoporosis. To find treatments that target both joint manifestations osteoporosis in desirable. The third generation selective oestrogen receptor modulators (SERMs) [lasofoxifene (LAS) bazedoxifene (BZA)] are new treatment options for aim this study was to investigate the effects LAS BZA on arthritic disease inflammation-associated bone loss using CIA mice. Methods. Female...
Abstract Estradiol (E2) is important for male skeletal health and the effect of E2 mediated via estrogen receptor (ER)-α. This was demonstrated by findings that men with an inactivating mutation in aromatase or a nonfunctional ERα had osteopenia continued longitudinal growth after sexual maturation. The aim present study to evaluate role different domains effects selective modulators (SERMs) on bone mass males. Three mouse models lacking either ERαAF-1 (ERαAF-10), ERαAF-2 (ERαAF-20), total...
Testosterone has profound immune-modulatory actions, which may be important for the sexual dimorphism in immune-related disorders, such as autoimmune diseases. A well-known effect of androgens is inhibition bone marrow B lymphopoiesis; however, a plausible target cell this not yet been presented. The aim study was to determine androgen-mediated regulation lymphopoiesis males. We confirm higher number cells male mice with global inactivation androgen receptor (AR) and these AR knockout...
Abstract In addition to the systemic inflammation present in rheumatoid arthritis (RA), decreased estradiol levels postmenopausal RA patients further accelerate bone loss these patients. The tissue-selective estrogen complex (TSEC), an combined with a selective receptor modulator, is new hormone replacement therapy option. first approved TSEC, containing conjugated estrogens and bazedoxifene (BZA), reduces menopausal symptoms prevents osteoporosis improved safety profile compared...
Abstract For conditions with inflammatory flare‐ups, fast drug‐release from a depot is crucial to reduce cell infiltration and prevent long‐term tissue destruction. While this concept has been explored for chronic diseases, preventing acute flares not explored. To address issue, preventative inflammation‐sensitive system developed applied gout, condition where millions of cells are recruited rapidly, causing excruciating debilitating pain. Rapid drug release first demonstrated pH‐responsive...
Dehydroepiandrosterone (DHEA) is an abundant steroid hormone, and its mechanism of action yet to be determined. The aim this study was elucidate the importance androgen receptors (ARs) estrogen (ERs) for DHEA function. Orchidectomized C57BL/6 mice were treated with DHEA, DHT, 17β-estradiol-3-benzoate (E2), or vehicle. AR-deficient (ARKO) wild-type (WT) littermates vehicle 2.5 weeks. At termination, bone mineral density (BMD) evaluated, thymus seminal vesicles weighted, submandibular glands...
Objective— Androgen deprivation therapy has been associated with increased cardiovascular risk in men. Experimental studies support that testosterone protects against atherosclerosis, but the target cell remains unclear. T cells are important modulators of and deficiency or its receptor, AR (androgen receptor), induces a prominent increase thymus size. Here, we tested hypothesis atherosclerosis induced by male mice is T-cell dependent. Further, given role thymic epithelium for homeostasis...