A5004847651- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Parkinson's Disease Mechanisms and Treatments
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Peroxisome Proliferator-Activated Receptors
- Immune cells in cancer
- Analytical Chemistry and Sensors
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Electrochemical sensors and biosensors
- Molecular Sensors and Ion Detection
- Cancer Research and Treatments
- Histone Deacetylase Inhibitors Research
- Eicosanoids and Hypertension Pharmacology
- Sulfur Compounds in Biology
- Inflammatory mediators and NSAID effects
- Coenzyme Q10 studies and effects
- Lung Cancer Treatments and Mutations
- Redox biology and oxidative stress
- Adenosine and Purinergic Signaling
- T-cell and B-cell Immunology
Agilent Technologies (United States)
2017-2023
Rogers (United States)
2019
Agilent Technologies (Switzerland)
2019
Agilent Technologies (Ireland)
2019
Agilent Technologies (Germany)
2018
Ferric (United States)
2017
Medical College of Wisconsin
2010-2015
University of Alabama at Birmingham
2008-2011
Mitochondria play a critical role in mediating the cellular response to oxidants formed during acute and chronic cardiac dysfunction. It is widely assumed that, as cells are subjected stress, mitochondria capable of drawing upon 'reserve capacity' which available serve increased energy demands for maintenance organ function, repair or detoxification reactive species. This hypothesis further implies that impairment depletion this putative reserve capacity ultimately leads excessive protein...
Cancer cells are long known to exhibit increased aerobic glycolysis, but glycolytic inhibition has not offered a viable chemotherapeutic strategy in part because of the systemic toxicity antiglycolytic agents. However, recent studies suggest that combined glycolysis and mitochondrial function may help overcome this issue. In study, we investigated efficacies mitochondria-targeted drugs (MTD) combination with 2-deoxy-d-glucose (2-DG), compound inhibits glycolysis. Using MTDs, termed Mito-CP...
Boronates, a group of organic compounds, are emerging as one the most effective probes for detecting and quantifying peroxynitrite, hypochlorous acid, hydrogen peroxide. Boronates react with peroxynitrite nearly million times faster than Boronate-containing fluorogenic compounds have been used to monitor real time generation in cells imaging peroxide living animals. This perspective highlights potential applications boronates other fluorescent high-throughput analyses hydroperoxides...
Herein we describe a high-throughput fluorescence and HPLC-based methodology for global profiling of reactive oxygen nitrogen species (ROS/RNS) in biological systems. The combined use HPLC detection is key to successful implementation validation this methodology. Included here are methods specifically detect quantitate the products formed from interaction between ROS/RNS fluorogenic probes, as follows: superoxide using hydroethidine, peroxynitrite boronate-based nitric oxide-derived...
Abstract Background Parkinson’s disease (PD) is a devastating neurodegenerative disorder characterized by progressive motor debilitation, which affects several million people worldwide. Recent evidence suggests that glial cell activation and its inflammatory response may contribute to the degeneration of dopaminergic neurons in PD. Currently, there are no neuroprotective agents available can effectively slow progression. Herein, we evaluated anti-inflammatory antioxidant efficacy diapocynin,...
Abstract Oxidative phosphorylation and glycolysis are the dominant ATP‐generating pathways in mammalian metabolism. The balance between these two is often shifted to execute cell‐specific functions response stimuli that promote activation, proliferation, or differentiation. However, measurement of metabolic switches has remained mostly qualitative, making it difficult discriminate healthy, physiological changes energy transduction compensatory responses due dysfunction. We therefore present...
Abnormal smooth muscle cell proliferation is a hallmark of vascular disease. Although growth factors are known to contribute hyperplasia, the changes in metabolism associated with this response, particularly mitochondrial respiration, remain unclear. Given increased energy requirements for proliferation, we hypothesized that PDGF (platelet-derived factor) would stimulate glycolysis and respiration elevated bioenergetic capacity required hyperplasia. To test hypothesis, glycolytic flux oxygen...
Docosahexaenoic acid (DHA; C22:6n−3) depresses mammary carcinoma proliferation and growth in cell culture animal models. The current study explored the role of interrupting bioenergetic pathways BT-474 MDA-MB-231 breast cancer lines representing respiratory glycolytic phenotypes, respectively comparing impacts DHA with a non-transformed line, MCF-10A. Metabolic investigation revealed that supplementation significantly diminished profile malignant dose-dependent manner. enrichment also...
In vitro and in vivo models of Parkinson's disease (PD) suggest that increased oxidant production leads to mitochondrial dysfunction dopaminergic neurons subsequent cell death. However, it remains unclear if death these is caused by inhibition function or production. The objective this study was determine the relationship between response multiple PD neurotoxicant mimetics. MPP(+) a dose-dependent decrease basal oxygen consumption rate N27 cells, indicating loss function. parallel, we found...
Abstract Thrombospondin‐1 (TSP‐1) treatment of dermal microvascular endothelial cells (MvEC) has been shown to upregulate Fas ligand (FasL) and induce apoptosis by a mechanism that requires caspase‐8 activity. We have examined the potential anti‐angiogenic effects TSP‐1 on primary human brain MvEC. The addition MvEC cultured as monolayers type 1 collagen, induced cell death (evidenced caspase‐3 cleavage) in dose‐ (5–30 nM) time‐dependent (maximal at 17 h) manner. for h cleavage required...
Recently, a number of steps in the progression metastatic disease have been shown to be regulated by redox signalling. Electrophilic lipids affect signalling through post-translational modification critical cysteine residues proteins. However, therapeutic potential as well precise mechanisms action electrophilic cancer cells is poorly understood. In present study, we investigate effect prostaglandin 15d-PGJ2 (15-deoxy-Δ12,14-prostaglandin J2) on properties breast cells. was decrease...
Abstract Fast proliferating cells require tight regulation to achieve a balance between the use of nutrients for ATP production (through glycolysis and oxidative phosphorylation) intermediate metabolites sustain increased biosynthetic activity. Cancer cells, but also high proliferative non-transformed exhibit glycolytic activity during rapid proliferation even in presence normal oxygen concentrations culture. However, despite activity, role is not necessary as major contributor allow...
ABSTRACT Oxidative phosphorylation and glycolysis are the dominant ATP-generating pathways in mammalian metabolism. The balance between these two is often shifted to execute cell-specific functions response stimuli that promote activation, proliferation, or differentiation. However, measurement of metabolic switches has remained mostly qualitative, making it difficult discriminate healthy, physiological changes energy transduction compensatory responses due dysfunction. We therefore...