A5006480671- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Tuberculosis Research and Epidemiology
- Advanced Glycation End Products research
- Metabolism, Diabetes, and Cancer
- Cardiovascular Disease and Adiposity
- Protein Hydrolysis and Bioactive Peptides
- Lipid metabolism and biosynthesis
- Growth Hormone and Insulin-like Growth Factors
- Regulation of Appetite and Obesity
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Ovarian function and disorders
- Pancreatic function and diabetes
- Protease and Inhibitor Mechanisms
- Adrenal Hormones and Disorders
- Pharmacology and Obesity Treatment
- Biochemical effects in animals
- Botulinum Toxin and Related Neurological Disorders
- Exercise and Physiological Responses
- Mycobacterium research and diagnosis
- Metabolomics and Mass Spectrometry Studies
- Head and Neck Anomalies
- Peroxisome Proliferator-Activated Receptors
- Vasculitis and related conditions
University of California, San Diego
2012-2024
Rady Children's Hospital-San Diego
2012-2024
Children's Hospital of Philadelphia
1989-2022
VA San Diego Healthcare System
2003-2022
Royal Liverpool and Broadgreen University Hospital NHS Trust
2015
Veterans Health Administration
2013
La Jolla Alcohol Research
2013
United States Department of Veterans Affairs
2010
Halstead Hospital
2003
UC San Diego Health System
2003
In Krebs-Ringer phosphate buffer, the rate of formation methylglyoxal from glycerone and glyceraldehyde 3-phosphate was first order with respect to triose rates constant values 1.94 +/- 0.02 x 10(-5) s-1 (n = 18) 1.54 10(-4) at 37 degrees C, respectively. The in presence red blood cell lysate not significantly different non-enzymatic value (P > 0.05). Methylglyoxal increased isomerase but this may be due faster isomerisation product. For cells vitro, predicted account for metabolic flux...
The relationship between insulin action and control of the adipocyte-derived factor adiponectin was studied in age- weight-matched obese individuals with type 2 diabetes failing sulfonylurea therapy. After initial metabolic characterization, subjects were randomized to troglitazone or metformin treatment groups; all received glyburide (10 mg BID) as well. Treatment continued for 3 months. extent glycemic after similar both groups. However, increase maximal insulin-stimulated glucose disposal...
Adiponectin is an insulin sensitizing fat cell (FC) hormone whose levels are related to adipose tissue (AT) mass and depot distribution. We hypothesized that the nature of AT expansion (hypertrophy vs. hyperplasia) contributes obesity-related reductions in serum adiponectin this effect influenced by regional distribution subcutaneous (S) visceral (V) depots. Thirteen obese subjects provided paired biopsies. Serum total high molecular weight (HMW) were determined ELISA. Secretion was...
Sgk (serum- and glucocorticoid-induced protein kinase) is a serine/threonine-specific kinase that transcriptionally regulated by serum, glucorticoids, mineralocorticoids. regulates the amiloride-sensitive sodium channel in kidney principal cells. Insulin insulin-like growth factor-1 stimulate activity of mechanism mediated phosphoinositide-dependent kinases (PDK)-1 -2. In this study, we demonstrate incubation transfected cells with 8-(4-chlorophenylthio)-cAMP (8CPT-cAMP; 0.2 mm) led to...
Circulating adiponectin is reduced in disorders associated with insulin resistance. This study was conducted to determine whether an exercise/diet intervention would alter multimer distribution and receptor expression skeletal muscle. Impaired glucose-tolerant older (>60 yr) obese (BMI 30–40 kg/m 2 ) men ( n = 7) women 14) were randomly assigned 12 wk of supervised aerobic exercise combined either a hypocaloric (ExHypo, ∼500 kcal reduction, 11) or eucaloric diet (ExEu, 10). Insulin...
Background: Well-differentiated thyroid cancer (WDTC) incidence in pediatrics is rising, most being papillary carcinoma (PTC). The objective of the study was to assess prevalence different mutations pediatric WDTC and correlate genotype with clinical phenotype. Methods: This a single-center retrospective study. Thyroid tissue blocks from 42 consecutive patients who underwent thyroidectomy between 2001 2013 were analyzed at Quest Diagnostics for BRAFV600E, RAS (N,K,H), RET/PTC PAX8/PPARγ...
Sorting nexins are a family of phox homology domain containing proteins that homologous to yeast involved in protein trafficking. We have identified novel 342-amino acid residue sorting nexin, SNX15, and 252-amino splice variant, SNX15A. Unlike many nexins, SNX15 ortholog has not been or Caenorhabditis elegans. By Northern blot analysis, mRNA is widely expressed. Although predicted be soluble protein, both endogenous overexpressed found on membranes the cytosol. The required for its membrane...
Polycystic ovary syndrome (PCOS) is characterized by insulin resistance, compensatory hyperinsulinemia, increased prevalence of impaired glucose tolerance, and ovarian androgen biosynthesis.The aim the study was to evaluate effects pioglitazone on whole body action biosynthesis in PCOS.We performed a randomized placebo-controlled trial.The conducted at Special Diagnostic Treatment Unit Veterans Affairs Medical Center, San Diego, University California, General Clinical Research Center.A total...
Abstract Objective : To determine whether adipocyte differentiation‐related protein (ADRP), a lipid droplet—associated that binds to and sequesters intracellular fatty acids, is 1) expressed in human skeletal muscle 2) differentially regulated obtained from obese non‐diabetic (OND) diabetic (OD) subjects. Research Methods Procedures Ten OND subjects 15 OD underwent weight loss or pharmacological intervention program improve insulin sensitivity. Anthropometric data, hemoglobin A 1C , fasting...
The subcutaneous (S) and visceral (V) adipose tissue (AT) depots are increasingly recognized as distinct. To test the hypothesis that depot differences exist for adiponectin, fresh cultured human VAT SAT from obese type 2 diabetic (T2D) nondiabetic (ND) subjects was examined to determine whether in adiponectin content secretion occurred a function of studied, status, response thiazolidinedione treatment. were obtained by biopsy AT explants defined media 7 days. Protein expression assessed...
Adiponectin, an insulin-sensitizing factor secreted from adipose tissue, is decreased in individuals with type 2 diabetes (T2D) and increased response to thiazolidinedione (TZD) therapy. Changes its secretion assembly into higher-order forms affect insulin sensitivity. To determine the relative potency of TZDs on intra-adipocyte multimerization adiponectin, we assessed impact vivo low- or high-dose rosiglitazone treatment alone combined metformin subjects T2D. T2D received (8 mg/day), (2,000...
Reduced capillary density is a feature of skeletal muscle (SkM) in type 2 diabetes (T2D), which associated with multiple metabolic and functional abnormalities. SkM has been identified as secretory tissue, releasing myokines that regulate processes, including vascularization. We sought to determine how secreted from T2D myotubes might influence angiogenesis. Conditioned media (CM) were generated by nondiabetic (ND) subjects. Primary human endothelial cells (HUVEC) explants exposed CM or...
Sorting nexin (SNX) 15 is a novel member of the SNX family proteins. Although functions most SNXs have not yet been determined, several members (e.g., SNX1, SNX2, SNX3, and SNX8) are orthologs yeast proteins involved in protein trafficking. Overexpression myc‐tagged SNX15 COS‐7 cells altered morphology endosomal compartments. In transient transfection experiments, myc‐SNX15 was first seen small punctate spots ring structures. Later, found larger rings. Finally, observed large, amorphous...