A5089658570- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- Fatty Acid Research and Health
- Extracellular vesicles in disease
- Infant Nutrition and Health
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Pancreatic function and diabetes
- Viral gastroenteritis research and epidemiology
- Breastfeeding Practices and Influences
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Nanopore and Nanochannel Transport Studies
- Peroxisome Proliferator-Activated Receptors
- Metabolomics and Mass Spectrometry Studies
- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Mitochondrial Function and Pathology
University of Michigan
2019-2024
BioSurfaces (United States)
2019-2024
University of California, San Diego
2016-2024
Michigan United
2024
La Jolla Bioengineering Institute
2016-2020
Mechanisms of metabolic flexibility enable cells to survive under stressful conditions and can thwart therapeutic responses. Acetyl-coenzyme A (CoA) plays central roles in energy production, lipid metabolism, epigenomic modifications. Here, we show that, upon genetic deletion Acly, the gene coding for ATP-citrate lyase (ACLY), remain viable proliferate, although at an impaired rate. In absence ACLY, upregulate ACSS2 utilize exogenous acetate provide acetyl-CoA de novo lipogenesis (DNL)...
Viruses are obligate intracellular parasites that rely on host cell metabolism for successful replication. Thus, viruses rewire pathways involved in central carbon to increase the availability of building blocks propagation. However, underlying mechanisms virus-induced alterations largely unknown. Noroviruses (NoVs) highly prevalent pathogens cause sporadic and epidemic viral gastroenteritis. In present study, we uncovered several strain-specific shared metabolic requirements three murine...
Background and Aims: TM6SF2 rs58542926 (E167K) is related to an increased prevalence of metabolic dysfunction–associated steatotic liver disease. Conflicting mouse study results highlight the need for a human model understand this mutation’s impact. This aims create characterize reliable in vitro mimic effects TM6SF2-E167K mutation future studies. Approach Results: We used gene editing on human-induced pluripotent stem cells from healthy individual with mutation. After hepatocyte-directed...
We sought to evaluate the relevance of pediatric dairy fat recommendations for children at risk nonalcoholic fatty liver disease (NAFLD) by studying association between intake and amount fat. The effects may be mediated odd chain acids (OCFA), such as pentadecanoic acid (C15:0), monomethyl branched (BCFA), iso-heptadecanoic (iso-C17:0). Therefore, we also evaluated plasma levels OCFA BCFA with
Abstract Polydimethylsiloxane (PDMS) is an inexpensive robust polymer that commonly used as the fundamental fabrication material for soft‐lithography‐based microfluidic devices. Owing to its versatile properties, there are some attempts use PDMS a porous 3D structure sensing. However, reliable and easy has been challenging along with inherent hydrophobic nature of hindering in biomedical sensing applications. Herein, cleanroom‐free method create structures, “ExoSponge” effective surface...
Breastfeeding is an important determinant of infant health and there immense interest in understanding its metabolite composition so that key beneficial components can be identified. The aim this research was to measure the fatty acid human milk Irish cohort where we examined changes depending on lactation stage gestational weight gain trajectory. Utilizing a chromatography approach optimal for isomer separation, identified 44 individual species via GCMS showed monomethyl branched-chain...
Abstract Objectives Pluripotent stem cell-derived cardiomyocytes are phenotypically immature, which limits their utility in downstream applications. Metabolism is dramatically reprogramed during cardiac maturation vivo and presents a potential avenue to drive vitro maturation. We aimed identify address metabolic bottlenecks the generation of human pluripotent cell (hPSC)-derived cardiomyocytes. Methods hPSCs were differentiated into using an established, chemically-defined differentiation...
Summary Tumor-driven immune suppression poses a significant impediment to the success of immunotherapy in ovarian cancer. Among various mechanisms contributing suppression, intracellular communication facilitated by tumor-derived extracellular vesicles (EVs) within tumor microenvironment (TME) emerges as pivotal factor influencing growth. We discovered that EVs from both cell lines and plasma cancer patients are encapsulated with eukaryotic translation initiation 4E (eIF4E). Our study...
Abstract Viruses are obligate intracellular parasites that rely on host cell metabolism for successful replication. Thus, viruses rewire pathways involved in central carbon to increase the availability of building blocks However, underlying mechanisms virus-induced alterations largely unknown. Noroviruses (NoVs) highly prevalent pathogens cause sporadic and epidemic viral gastroenteritis. In present study, we uncovered several strain-specific shared metabolic requirements three murine...
Abstract Breastfeeding is an important determinant of infant health and there immense interest in understanding its metabolite composition so that key beneficial components can be identified. The aim this research was to measure the fatty acid human milk Irish cohort where we examined changes depending on lactation stage gestational weight gain trajectory. Utilising a chromatography approach optimal for isomer separation, identified 44 individual species via GCMS showed monomethyl...
Abstract Large-scale chromosomal alterations, particularly deletions in cancer genomes confer functional advantages to cells via the loss of tumor suppressor genes (TSGs). However, due nature these focal and arm-level deletions, essential house-keeping neighborhood TSGs are potentially lost. We explore emergence metabolic adaptations vulnerabilities that arise collateral genes. In our previous work, we showed genomic locus containing SMAD4 ME2 pancreatic ductal adenocarcinomas forces rely on...