A5004211471- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Viral gastroenteritis research and epidemiology
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Sirtuins and Resveratrol in Medicine
- Lipid metabolism and biosynthesis
- Growth Hormone and Insulin-like Growth Factors
- Metabolism, Diabetes, and Cancer
- Pituitary Gland Disorders and Treatments
- Bacteriophages and microbial interactions
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Immune cells in cancer
- Cholesterol and Lipid Metabolism
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Body Composition Measurement Techniques
University of Pennsylvania
2016-2020
Cancer Research Institute
2016-2019
Cancer Research Institute of the Slovak Academy of Sciences
2017
National Center for Infectious Diseases
2015
Stony Brook University
2011-2012
Universidad Complutense de Madrid
1994
Mechanisms of metabolic flexibility enable cells to survive under stressful conditions and can thwart therapeutic responses. Acetyl-coenzyme A (CoA) plays central roles in energy production, lipid metabolism, epigenomic modifications. Here, we show that, upon genetic deletion Acly, the gene coding for ATP-citrate lyase (ACLY), remain viable proliferate, although at an impaired rate. In absence ACLY, upregulate ACSS2 utilize exogenous acetate provide acetyl-CoA de novo lipogenesis (DNL)...
Cellular metabolism dynamically regulates the epigenome via availability of metabolite substrates chromatin-modifying enzymes. The impact diet on metabolism-epigenome axis is poorly understood but could alter gene expression and influence metabolic health. ATP citrate-lyase produces acetyl-CoA in nucleus cytosol histone acetylation levels many cell types. Consumption a high-fat (HFD) results suppression tissues such as adipose liver, these remains unknown. Here we examined effects HFD...
mTORC2 phosphorylates AKT in a hydrophobic motif site that is biomarker of insulin sensitivity. In brown adipocytes, regulates glucose and lipid metabolism, however the mechanism has been unclear because downstream signaling appears unaffected by loss. Here, applying immunoblotting, targeted phosphoproteomics metabolite profiling, we identify ATP-citrate lyase (ACLY) as distinctly mTORC2-sensitive substrate preadipocytes. dispensable for most other actions examined, indicating previously...
Sugars and refined carbohydrates are major components of the modern diet. ATP-citrate lyase (ACLY) is upregulated in adipocytes response to carbohydrate consumption generates acetyl-coenzyme A (CoA) for both lipid synthesis acetylation reactions. Here, we investigate role ACLY metabolic transcriptional responses unexpectedly uncover a sexually dimorphic function maintaining systemic homeostasis. When fed high-sucrose diet, AclyFAT−/− females exhibit lipodystrophy-like phenotype, with minimal...
Abstract Salmonellae are pathogenic bacteria that induce immunosuppression by mechanisms remain largely unknown. Previously, we showed a putative type II l-asparaginase produced Salmonella Typhimurium inhibits T cell responses and mediates virulence in murine model of infection. Here, report this exhibits l-asparagine hydrolase activity required for to inhibit cells. We show is nutrient important activation deprivation, such as mediated the l-asparaginase, causes suppression...
Sugars and refined carbohydrates are major components of the modern diet. ATP-citrate lyase (ACLY) is upregulated in adipocytes response to carbohydrate consumption generates acetyl-CoA for both lipid synthesis acetylation reactions. Here we investigated role ACLY metabolic transcriptional responses adipocytes, unexpectedly uncovered a sexually dimorphic function maintaining systemic homeostasis. When fed high sucrose diet, AclyFAT-/- females exhibit lipodystrophy-like phenotype, with...
Abstract T cells play a key role in controlling and clearing infection with the bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium). However, cell-mediated immunity against S. takes months to develop has been described as slow inefficient. Previously, we have shown that direct inhibitory effect on cells, down-modulating expression of beta chain cell receptor (TCR-beta) inhibiting proliferation. Furthermore, soluble, proteinaceous factor produced or induced by is...