A5101566451- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Immune cells in cancer
- Cancer Mechanisms and Therapy
- PI3K/AKT/mTOR signaling in cancer
- Crystallography and molecular interactions
- RNA Research and Splicing
- Curcumin's Biomedical Applications
- RNA regulation and disease
- Nanoparticle-Based Drug Delivery
- Cancer-related Molecular Pathways
- Brain Tumor Detection and Classification
- Ubiquitin and proteasome pathways
- Tannin, Tannase and Anticancer Activities
- Nuclear Structure and Function
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Synthesis and biological activity
- Artificial Intelligence in Healthcare
- Genomics, phytochemicals, and oxidative stress
Medical College of Wisconsin
2020-2024
Chitkara University
2024
Jawaharlal Nehru Medical College
2024
Medical College of Wisconsin Cancer Center
2024
Jawaharlal Nehru University
2015-2022
University of Wisconsin–Milwaukee
2022
Kidwai Memorial Institute of Oncology
2013
Maharshi Dayanand University
2012
Abstract SPHK1 (sphingosine kinase‐1) catalyzes the phosphorylation of sphingosine to sphingosine‐1‐phosphate (S1P), is found be highly expressed in solid tumors. Here, extracellular vesicles (EVs) are identified as key transporters tumor microenvironment. Consequently, SPHK1‐packaged EVs elevate S1P levels microenvironment, where appears an immunosuppressive agent. However, exact mechanism how mediates its effects cancer not understood. It investigated that can induce T cell exhaustion....
Fragile X-related protein-1 (FXR1) gene is highly amplified in patients with ovarian cancer, and this amplification associated increased expression of both FXR1 mRNA protein. directly associates the survival proliferation cancer cells. Surface sensing translation (SUnSET) assay demonstrates that enhances overall Reverse-phase protein array (RPPA) reveals cMYC key target FXR1. Mechanistically, binds to AU-rich elements (ARE) present within 3' untranslated region (3'UTR) stabilizes its...
Ovarian cancer is the most lethal gynecological malignancy among women worldwide and characterized by aggressiveness, stemness, frequent relapse due to resistance platinum-based therapy. cells metastasize through ascites fluid as 3D spheroids which are more resistant apoptosis chemotherapeutic agents. However, precise mechanism an oncogenic addiction that makes agents not understood. To study signaling occurs during progression in patients, we developed endometrioid subtype ovarian cell line...
Abstract Chemotherapy such as cisplatin is widely used to treat ovarian cancer either before or after surgical debulking. However, relapse due chemotherapy resistance a major challenge in the treatment of cancer. The underlying mechanisms related remain largely unclear. Therefore, identification effective therapeutic strategies urgently needed overcome therapy resistance. Transcriptome-based analysis, vitro studies and functional assays identified that cisplatin-resistant cells express high...
Conventional proximity ligation assay (PLA) suffers from target specificity issues that curtail their accuracy on interpreting proximal interactions in cell biology. Here, we present a reliable and sensitive approach by including fluorochrome-labeled mRNA fragment along with biotin-labeled RNA probe target-specific antibody, which were used to generate signals through linear connectors intact cells. This protocol will be particularly useful for studying the between binding proteins (RBPs)...
The increased tyrosine kinase activity of non‑small cell lung cancer (NSCLC)‑associated epidermal growth factor receptor (EGFR) mutants results in deregulated pathways that contribute to malignant survival, tumor progression and metastasis. Previous studies investigating cancer‑associated EGFR have focused on the prognostic implications mutations patients with NSCLC; however, role invasion migration remains undetermined. present study was designed investigate NSCLC‑associated mutant...
Extracellular vesicles (EVs) play a crucial role in facilitating communication between cancer cells and their immediate or remote microenvironments, thereby promoting the extensive spread of throughout body. In this context, we present protocol for isolation tumor cell-derived EVs followed by vivo metastasis assessment murine ovarian model. We describe steps characterization from ID8 cells, development metastatic mouse model, sample preparation flow cytometry. For complete details on use...