A5054461917- Ion Transport and Channel Regulation
- Hormonal Regulation and Hypertension
- Metabolism, Diabetes, and Cancer
- Ion channel regulation and function
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Pancreatic function and diabetes
- MicroRNA in disease regulation
- PI3K/AKT/mTOR signaling in cancer
- Galectins and Cancer Biology
- Protein Kinase Regulation and GTPase Signaling
- Genetics and Neurodevelopmental Disorders
- Genetic factors in colorectal cancer
- Protein Tyrosine Phosphatases
- ATP Synthase and ATPases Research
- Cancer-related Molecular Pathways
- Renin-Angiotensin System Studies
- Autophagy in Disease and Therapy
- Apelin-related biomedical research
- Growth Hormone and Insulin-like Growth Factors
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Thyroid Disorders and Treatments
- Skin and Cellular Biology Research
- DNA Repair Mechanisms
Magna Graecia University
2015-2024
Azienda Ospedaliero Universitario Mater Domini
2005-2023
Creative Research Enterprises (United States)
2016
University of Naples Federico II
1984-2015
National Institute of Diabetes and Digestive and Kidney Diseases
1985-2002
United States Nuclear Regulatory Commission
2002
Office of Extramural Research
2001
University of Reggio Calabria
1992-1998
National Institutes of Health
1986-1990
Tumori Foundation
1989
We report a PCR-based technique for detecting thyroid cancer metastases in small nodes <1.5 cm diameter by the amplification of specific transcripts TSH-receptor and thyroglobulin. A 100% correspondence with histopathological diagnosis was observed 41/46 (89%) which an adequate sample obtained at fine needle aspiration. The genetic analysis that resulted more sensitive accurate than both cytological (28% inadequate samples, 17% false negative diagnoses) thyroglobulin measurement aspirates...
Sgk (serum- and glucocorticoid-induced protein kinase) is a serine/threonine-specific kinase that transcriptionally regulated by serum, glucorticoids, mineralocorticoids. regulates the amiloride-sensitive sodium channel in kidney principal cells. Insulin insulin-like growth factor-1 stimulate activity of mechanism mediated phosphoinositide-dependent kinases (PDK)-1 -2. In this study, we demonstrate incubation transfected cells with 8-(4-chlorophenylthio)-cAMP (8CPT-cAMP; 0.2 mm) led to...
To study the role of sgk (serum, glucocorticoid-induced kinase) in hormonal regulation Na + transport mediated by epithelial channel (ENaC), clonal cell lines stably expressing human sgk, an S422A mutant, or a D222A mutant were created background A6 model renal line. Expression normal sgkresults 3.5-fold enhancement basal and potentiation natriferic response to antidiuretic hormone (ADH). Transfection form which cannot be phosphorylated phosphatidylinositol-dependent kinase (PDK)-2, results...
The insulin receptor possesses protein kinase activity, which may play a role in mediating action. Recently, we have identified glycoprotein (pp120) rat liver plasma membranes that is phosphorylated by the solubilized cell-free system. We now report stimulates phosphorylation of pp120 intact H-35 cells. cells were preloaded with [32P]orthophosphate to label intracellular ATP pool. Insulin caused 10-fold increase its and 2-fold (P less than 0.001). time course insulin's stimulation closely...
Abstract Background Lung cancer represents the most frequent cause of death for cancer. In non-small cell lung (NSCLC), which accounts vast majority this disease, only early detection and treatment, when possible, may significantly affect patient's prognosis. An important role in NSCLC malignancy is attributed to signal transduction pathways involving PI3Kinase, with consequent activation AKT family factors. The serum glucocorticoid kinase (SGK) factors, share high structural functional...
// Cristina Talarico 1 , Vincenzo Dattilo Lucia D’Antona Agnese Barone 2 Nicola Amodio Stefania Belviso Francesca Musumeci 3 Claudia Abbruzzese 5 Cataldo Bianco Francesco Trapasso Silvia Schenone Stefano Alcaro Ortuso Tullio Florio 4 Marco G. Paggi Perrotti and Rosario Amato Department of “Scienze della Salute”, University ”Magna Graecia” Catanzaro, Italy “Medicina Sperimentale e Clinica”, Farmacia, Genova, Medicina Interna Specialità Mediche...
// Cristina Talarico 1, * , Lucia D'Antona Domenica Scumaci 2 Agnese Barone Francesco Gigliotti 1 Claudia Vincenza Fiumara Vincenzo Dattilo Enzo Gallo 3 Paolo Visca Ortuso Abbruzzese 4 Lorenzo Botta 5 Silvia Schenone 6 Giovanni Cuda Stefano Alcaro Cataldo Bianco Patrizia Lavia 7 Marco G. Paggi Nicola Perrotti Rosario Amato Department of "Scienze della Salute", University "Magna Graecia" Catanzaro, Viale Europa, Italy "Medicina Sperimentale e Clinica", Section Pathology, Regina Elena National...
Background/Aims: Published observations on serum and glucocorticoid regulated kinase 1 (Sgk1) knockout murine models Sgk1-specific RNA silencing in the RKO human colon carcinoma cell line point to this as a central player carcinogenesis resistance taxanes. Methods: By vitro activity inhibition assays, cycle viability analysis cancer model systems, we describe biologic effects of recently identified inhibitor, SI113, characterized by substituted pyrazolo[3,4-d]pyrimidine scaffold, that shows...
Endometrial cancer is often characterized by PI3K/AKT pathway deregulation. Recently it has been suggested that SGK1, a serine/threonine protein kinase shares structural and functional similarities with the AKT family, might play role in cancer, since its expression and/or activity found to be deregulated different human tumors. However, of SGK1 endometrial poorly investigated. Here, we show increased tissue specimens from neoplastic endometrium. The inhibitor SI113 induced significant...
The ability of pathogens to sequester iron from their host cells and proteins affects virulence. Moreover, is required for various innate defense mechanisms as well acquired immune responses. Therefore, intracellular concentration may influence the interplay between system. Here, we investigated whether changes in concentrations ferritin heavy chain (FTH) abundance modulate expression Major Histocompatibility Complex molecules (MHC) susceptibility Natural Killer (NK) cell cytotoxicity. FTH...
Reactive oxygen species play a critical role in inducing apoptosis. The small GTPase p21 Ras and the ERK1/2 MAPK have been proposed as key regulators of signaling cascade triggered by oxidative stress (H2O2). Harvey-Ras (Ha-Ras) Kirsten-Ras (Ki-Ras) isoforms are so far functionally indistinguishable, because they activate same downstream effectors, including ERK1/2. Moreover, has involved both protection induction apoptosis.Human umbilical vein endothelial cells (HUVECs) were subjected to...
Abstract —The response of the forearm vasculature to acetylcholine (7.5, 15, and 30 μg/min, each for 5 minutes) sodium nitroprusside (0.8, 1.6, 3.2 was evaluated in 32 never-treated hypertensive outpatients (17 men 15 women, aged 43±7 years) 24 normotensive control subjects (14 10 42±6 years). Drugs were infused into brachial artery, blood flow measured by strain-gauge plethysmography. In both groups, a deletion ( D )/insertion I ) polymorphism intron 16 angiotensin-converting enzyme ACE...
The effect of hyperglycemia per se on glucose uptake by muscle tissue was quantitated in six controls and type II diabetics the forearm technique, under conditions insulin deficiency induced somatostatin (SRIF) infusion (0.7 mg/h). Blood concentration clamped at its basal value during first 60 min SRIF then raised to approximately 200 mg/dl a variable infusion. Plasma levels remained or below 5 microU/ml infusion, including hyperglycemic period. No appreciable difference between present...
Abstract The serum and glucocorticoid inducible kinase SGK1 was originally cloned from mammary tumor cells. found to be up‐regulated in a variety of tumors, but down‐regulated several distinct tumors. Thus, evidence for role growth remained conflicting. According vitro observations, is by the oncogene β‐catenin negatively regulates proapoptotic transcription factor FOXO3a, which turn stimulates Bcl2‐interacting mediator BIM. This study aimed define colon carcinoma vivo . knockout mice ( sgk1...
The serum/glucocorticoid-inducible kinase 1 (Sgk1) has demonstrated antiapoptotic function and the capability to regulate cell survival, proliferation, differentiation. A pivotal role of Sgk1 in carcinogenesis resistance anticancer therapy been suggested. With aim identifying new modulators, 322 pyrazolo-pyrimidine derivatives have virtually screened with respect a crystallographic model Sgk1. top five ranked compounds evaluated demonstrating inhibition vitro selectivity compared RAC-alpha...