A5005017125- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- melanin and skin pigmentation
- Cervical Cancer and HPV Research
- Autophagy in Disease and Therapy
- Protein Structure and Dynamics
- Genital Health and Disease
- Glioma Diagnosis and Treatment
- Sirtuins and Resveratrol in Medicine
- Hippo pathway signaling and YAP/TAZ
- Brain Metastases and Treatment
- Cancer, Lipids, and Metabolism
- Cynara cardunculus studies
- Endoplasmic Reticulum Stress and Disease
- Pancreatic and Hepatic Oncology Research
- RNA and protein synthesis mechanisms
- Genomics, phytochemicals, and oxidative stress
- Photoreceptor and optogenetics research
- Skin Protection and Aging
- Receptor Mechanisms and Signaling
Centro di Riferimento Oncologico della Basilicata
2018-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2023
University of Florence
2007-2008
Istituto Pasteur
1996-2002
University of L'Aquila
1999
Istituto per le Tecnologie Didattiche
1996-1998
Instituto di Biofisica
1990-1998
University of Rome Tor Vergata
1992
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
1990-1992
Cancer Institute (WIA)
1986-1988
We investigated several phenotypic and functional parameters of T cell-mediated immunity in a large series common variable immunodeficiency (CVID) patients. demonstrated that the vast majority CVID patients presented multiple cell abnormalities intimately related among them, severity which was reflected parallel loss CD4+ naive cells. A strong correlation between number cells clinical features observed, supporting subgrouping according to their lymphocytes. reduced thymic output disrupted...
Cathepsins represent a group of proteases involved in determining the metastatic potential cancer cells. Among these are cysteinyl- (e.g. cathepsin B and L) aspartyl-proteases D), normally present inside lysosomes as inactive proenzymes. Once released extracellular space, cathepsins contribute to by facilitating cell migration invasiveness. In work we first evaluated, vitro procedures, role B, L D, remodeling, spreading invasiveness eight different lines: four primary melanoma lines. these,...
The human breast cancer cell line, estrogen receptor negative, MDA-MB231, was used to evaluate the antitumor effect of polyphenolic extracts from edible part artichokes (AEs). Treatment cells reduced viability and inhibited growth in a dose-dependent manner. Importantly, AEs did not have any on normal epithelial MCF10A. Chlorogenic acid (ChA), most representative component fraction artichoke, had no prominent effects death rate MDA-MB231 cells. addition cells, rather than ChA, triggered...
Summary UV‐induced DNA damage can lead to melanoma, the most dangerous form of skin cancer. Understanding mechanisms employed by melanocytes protect against UV is therefore a key issue. In melanocytes, catalase main enzyme responsible for degrading hydrogen peroxide and we have previously shown that low basal levels activity are associated with light phototype in vitro ex vivo models. Here investigate possible correlation between its melanogenesis primary cultures human melanocytes. We show...
Abstract Background Lung cancer represents the most frequent cause of death for cancer. In non-small cell lung (NSCLC), which accounts vast majority this disease, only early detection and treatment, when possible, may significantly affect patient's prognosis. An important role in NSCLC malignancy is attributed to signal transduction pathways involving PI3Kinase, with consequent activation AKT family factors. The serum glucocorticoid kinase (SGK) factors, share high structural functional...
Polyphenolic extracts from the edible part of artichoke ( Cynara scolymus L.) have been shown to be potential chemopreventive and anticancer dietary compounds. High doses polyphenolic (AEs) induce apoptosis decrease invasive human breast cancer cell line, MDA-MB231. However, molecular mechanism underlying AEs antiproliferative effects is not completely understood. We demonstrate that chronic low treatment at sublethal concentrations suppress growth via a caspases-independent mechanism....
WE report here that, in cultured cerebellar granule cells, the CXC chemokine GRO β stimulates signaling pathway of extracellular signal-regulated kinases, and enhances both evoked spontaneous post-synaptic currents patch clamped Purkinje neurons from rat slices. The β-induced enhancement excitatory by stimulating parallel fibres is blocked inhibitor kinases PD98059, which also reduces basal frequency mean amplitude currents. Our results suggest that modulates neurotransmitter release...
A study was made of the effects 5-hydroxytryptamine (5HT) on homomeric neuronal nicotinic receptors (nAcChoR) expressed in Xenopus oocytes after injection cDNA encoding wild-type chicken alpha(7) subunit. Acetylcholine (AcCho) elicited large currents (IAcCho) that were reduced by 5HT a reversible and dose-dependent manner, with half-inhibitory concentration (IC50) 56 microM Hill coefficient (nH) 1.2. The inhibition IAcCho noncompetitive voltage independent, behavior incompatible channel...
Glioblastoma multiforme (GBM), due to its location, aggressiveness, heterogeneity and infiltrative growth, is characterized by an exceptionally dismal clinical outcome. The small molecule SI113, recently identified as a SGK1 inhibitor, has proven be effective in restraining GBM growth vitro vivo, showing also encouraging results when employed combination with other antineoplastic drugs or radiotherapy. Our aim was explore the pharmacological features of SI113 cells order elucidate pivotal...
Article10 September 2020Open Access Source DataTransparent process The actin modulator hMENA regulates GAS6-AXL axis and pro-tumor cancer/stromal cell cooperation Roberta Melchionna Tumor Immunology Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute, Rome, Italy Search for more papers by this author Sheila Spada Francesca Di Modugno Daniel D'Andrea Department of Medicine, Centre Cell Signaling Inflammation, Imperial College London, UK Anna Carlo Mariangela Panetta Maria Mileo...
The adenoviral E1A proteins are able to promote proliferation and transformation, inhibit differentiation, induce apoptosis, suppress tumor growth. extreme N terminus conserved region one of E1A, which indispensable for transcriptional regulation binding p300/CBP, TBP, pCAF, play essential roles in these abilities. These observations strongly suggest an intrinsic link between E1A-mediated other effects. In this report, we show that inhibits the normal growth Saccharomyces cerevisiae HF7c,...
NACHRS heterologously expressed in human cells after transient transfection with α3β4α5 or α3β4 subunit cDNAs exhibited similar sensitivities to antagonists and comparable functional channel profiles. However, the sum of two Hill equations was required for best fitting ACh dose—current response curves co-expression α5, α3 β4 subunits. One component that obtained α3β4-transfected cells, while additional component, putatively attributed an nAChR population, showed a coefficient > 2 nine-fold...
Human Papillomavirus (HPV)-16 is a paradigm for "high-risk" HPVs, the causative agents of virtually all cervical carcinomas. HPV E6 and E7 viral genes are usually expressed in these tumors, suggesting key roles their gene products, oncoproteins, inducing malignant transformation.By protein-protein interaction analysis, using mass spectrometry, we identified glutathione S-transferase P1-1 (GSTP1) as novel cellular partner HPV-16 oncoprotein. Following mapping region sequence that involved...
// Claudia Abbruzzese 1, * , Giada Catalogna 2, Enzo Gallo 3 Simona di Martino Anna M. Mileo 4 Mariantonia Carosi Vincenzo Dattilo 2 Silvia Schenone 5 Francesca Musumeci Patrizia Lavia 6 Nicola Perrotti Rosario Amato and Marco G. Paggi 1 Department of Research, Advanced Diagnostics Technological Innovation, Unit Cellular Networks Therapeutic Targets, "Regina Elena" National Cancer Institute, IRCCS, Rome, Italy "Scienze della Salute", University "Magna Graecia" Catanzaro, Pathology, Tumor...