A5081828201- melanin and skin pigmentation
- Skin Protection and Aging
- Acne and Rosacea Treatments and Effects
- Dermatology and Skin Diseases
- Biochemical Analysis and Sensing Techniques
- Retinoids in leukemia and cellular processes
- Peroxisome Proliferator-Activated Receptors
- Psoriasis: Treatment and Pathogenesis
- Dermatologic Treatments and Research
- Pharmacological Effects of Natural Compounds
- RNA regulation and disease
- Antioxidant Activity and Oxidative Stress
- Asthma and respiratory diseases
- Melanoma and MAPK Pathways
- Adipose Tissue and Metabolism
- Urticaria and Related Conditions
- Circadian rhythm and melatonin
- Cutaneous Melanoma Detection and Management
- Mast cells and histamine
- Biomedical Research and Pathophysiology
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Telomeres, Telomerase, and Senescence
- Sirtuins and Resveratrol in Medicine
- Nonmelanoma Skin Cancer Studies
San Gallicano Hospital
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2025
Brigham and Women's Hospital
2020
Federation of American Societies for Experimental Biology
2020
Rockefeller University
2020
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Inserm
1996-2012
Centre Méditerranéen de Médecine Moléculaire
2012
The synthesis of melanin pigments, or melanogenesis, is regulated by the balance a variety signal transduction pathways. Among these pathways, p38 MAPK signaling was found to be involved in stress-induced melanogenesis and activated alpha-melanocyte-stimulating hormone (alpha-MSH) ultraviolet irradiation. Previous studies have shown that alpha-MSH-stimulated can inhibited blocking activity with SB203580, pyridinyl imidazole compound. Consistent this, we observed imidazoles (SB203580...
Summary UV‐induced DNA damage can lead to melanoma, the most dangerous form of skin cancer. Understanding mechanisms employed by melanocytes protect against UV is therefore a key issue. In melanocytes, catalase main enzyme responsible for degrading hydrogen peroxide and we have previously shown that low basal levels activity are associated with light phototype in vitro ex vivo models. Here investigate possible correlation between its melanogenesis primary cultures human melanocytes. We show...
The MET proto-oncogene encodes for the hepatocyte growth factor (HGF) receptor, a plasma membrane tyrosine kinase that is involved in melanocyte and melanoma development. In mouse cells, Met expression increased by alphaMSH via activation of cAMP pathway. However, mechanism which regulates biological consequences this increase were not known. present report, we show both human melanocytes cells through transcriptional requires MITF. Furthermore, adenovirus driven MITF sufficient to cells....
Melasma is a hyperpigmentary disorder with photoaging features, whose manifestations appear on specific face areas, rich in sebaceous glands (SGs). To explore the SGs possible contribution to onset, expression of pro-melanogenic and inflammatory factors from SZ95 SG cell line exposed single or repetitive ultraviolet (UVA) radiation was evaluated. UVA up-modulated long-lasting production α-MSH, EDN1, b-FGF, SCF, cytokines mediators. Irradiated sebocyte conditioned media increased pigmentation...
Abnormalities of keratinocyte differentiation and impairment permeability barrier are features inflammatory skin diseases driven by Th1/Th17 Th2 immune response, such as psoriasis atopic dermatitis. We aimed at identifying the signature environments on keratinocytes, focusing expression genes involved in lipid metabolism profiles abundance metabolites. Human immortalized keratinocytes prodifferentiative conditions induced increasing calcium concentration, 3D epidermal equivalents were...
Cystinosis is a rare autosomal recessive disease characterized by cystine crystal accumulation leading to multiorgan dysfunctions and caused mutation in CTNS. CTNS encodes cystinosin, cystine/H+ symporter that exports out of the lysosomes. Patients with cystinosis frequently exhibit blond hair fair complexion, suggesting an alteration melanogenesis. However, pigmentation singularities these patients have not been studied, role cystinosin melanogenesis has remained unknown. In our study,...
Summary Given the importance of tanning response in protecting human skin from harmful effects UV radiation, one important research priority is to identify novel molecules that are capable promoting pigmentation and/or antioxidant defence. Parrodienes share some structural features with carotenoids and retinoids, stimulate cell defence counteract senescence‐like phenotype fibroblasts. We selected parrodiene‐derivative 2,4,6‐octatrienoic acid (Octa) study its impact on key parameters...
Abstract Azelaic acid (AzA) has been used for the treatment inflammatory skin diseases, such as acne and rosacea. Interestingly, an improvement in texture observed after long‐time with AzA. We previously unrevealed that anti‐inflammatory activity of AzA involves a specific activation PPARγ, nuclear receptor plays relevant role inflammation even ageing processes. As rosacea considered photo‐aggravated disease, we investigated ability to counteract stress‐induced premature cell senescence...
Abstract Purpose: Employing an in vitro model system of human melanoma progression, we previously reported ferritin light chain (L-ferritin) gene overexpression the metastatic phenotype. Here, attempted to characterize role biology and progression this disease. Experimental Design: Starting from LM cell line, engineered clones which L-ferritin expression was down-regulated by stable a specific antisense construct. These cells were then assayed for their growth capabilities, chemoinvasive...
Abstract Our aim was 2‐fold: to investigate the role of α‐tocopherol supplementation on antitumor activity DDP and evaluate effect survival neurotoxicity DDP‐treated mice. Experiments performed M14 human melanoma line demonstrated that did not influence efficacy DDP; inhibition cell in vivo tumor growth after treatment with combination similar observed alone. Conversely, also able increase mice treated a high dose DDP. While alone produced death about 70% mice, reduced deaths 30%. Analysis...
Abstract Background Acne, a disease of the sebaceous gland with multifactorial pathogenesis, affects more than 85% adolescents. A better deepening mechanisms underlying is needed to define effective and mechanism‐targeted treatments. Objective To understand whether sebocyte differentiation process could be involved in pathogenesis disease. Methods Protein expressions were evaluated by Western blot analysis ELISA ; mRNA levels real‐time RT ‐ PCR , lipid peroxidation performed gas...
Acne is the most common skin disease in adolescent Westernized populations. Several data support involvement of mammalian target rapamycin complex 1 (mTORC1) signalling interplay between androgens, insulin, insulin-like growth factor (IGF1) and high-glycaemic index diet acne. The peroxisome proliferator-activated receptor γ (PPARγ) involved both differentiation anti-inflammatory response. Low differentiated sebocytes showed decreased expression PPARγ increased level insulin IGF-1 receptors,...
We demonstrated a direct correlation between melanogenic and catalase activities on in vitro ex vivo models. Here, we investigated whether the stimulation of Melanocortin-1 Receptor (MC1R) could influence expression, activity cellular localization. For this purpose, treated B16-F0 melanoma cells with alpha-Melanocyte Stimulating Hormone (alpha-MSH) showed rapid induction through cAMP/PKA-dependent, microphthalmia-associated transcription factor (MITF) independent mechanism, acting at...
Peroxisome proliferator-activated receptor gamma (PPARγ) may be involved in a key mechanism of the skin aging process, influencing several aspects related to age-related degeneration cells, including antioxidant unbalance. Therefore, we investigated whether up-modulation this nuclear exerts protective effect stress-induced premature senescence (SIPS) model based on single exposure human dermal fibroblasts 8-methoxypsoralen plus + ultraviolet-A-irradiation (PUVA). Among possible PPARγ...