A5041216199- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Cancer-related gene regulation
- TGF-β signaling in diseases
- DNA Repair Mechanisms
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
- Muscle Physiology and Disorders
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Estrogen and related hormone effects
- Cardiac Fibrosis and Remodeling
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Wound Healing and Treatments
- Wnt/β-catenin signaling in development and cancer
- Immunotherapy and Immune Responses
Istituti di Ricovero e Cura a Carattere Scientifico
2005-2024
Istituto Dermopatico dell'Immacolata
2002-2013
Istituto Superiore di Sanità
2002
Scripps Research Institute
2000-2001
Sapienza University of Rome
1998-2000
Istituto Pasteur
1998
MicroRNAs (miRNAs) are small non-protein-coding RNAs that function as negative gene expression regulators. In the present study, we investigated miRNAs role in endothelial cell response to hypoxia. We found of miR-210 progressively increased upon exposure overexpression normoxic cells stimulated formation capillary-like structures on Matrigel and vascular growth factor-driven migration. Conversely, blockade via anti-miRNA transfection inhibited by hypoxia decreased migration factor. did not...
Tertiary Lymphoid Structures (TLS) correlate with positive outcomes in patients NSCLC and the efficacy of immune checkpoint blockade (ICB) cancer. The actin regulatory protein hMENA undergoes tissue-specific splicing, producing epithelial
Abstract Background Several cytokines are associated with the development and/or progression of chronic heart failure (CHF). Our aim was to look more closely at cytokine networks involved in CHF, and assess whether disease etiology affects expression. The study population comprised a) 69 patients stable New York Heart Association (NYHA) II/IV classes, secondary ischaemic (ICM) non dilated (NIDCM) cardiomyopathy b) 16 control subjects. We analyzed compared plasma levels 27 pro-...
The present study was aimed at identifying chronic heart failure (CHF) biomarkers from peripheral blood mononuclear cells (PBMCs) in patients with ischemic (ICM) and nonischemic dilated (NIDCM) cardiomyopathy. PBMC gene expression profiling performed by Affymetrix two patient groups, 1) ICM ( n = 12) 2) NIDCM New York Heart Association (NYHA) III/IV CHF patients, vs. 3) age- sex-matched control subjects 12). Extracted RNAs were then pooled hybridized to a total of 11 microarrays. Gene...
Transforming growth factor β (TGF-β) was found to inhibit differentiation of myogenic cells only when they were grown high density. Inhibition also occurred cocultured with other types mesenchymal but not epithelial cells. It is therefore possible that some density-dependent signaling mediates the intracellular response TGF-β. Within 30 min treatment, TGF-β induced translocation MEF2, MyoD, myogenin, or p21, cytoplasm Translocation reversible on withdrawal By using immune electron microscopy...
Article10 September 2020Open Access Source DataTransparent process The actin modulator hMENA regulates GAS6-AXL axis and pro-tumor cancer/stromal cell cooperation Roberta Melchionna Tumor Immunology Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute, Rome, Italy Search for more papers by this author Sheila Spada Francesca Di Modugno Daniel D'Andrea Department of Medicine, Centre Cell Signaling Inflammation, Imperial College London, UK Anna Carlo Mariangela Panetta Maria Mileo...
We have previously identified a muscle-specific enhancer within the first intron of human β enolase gene. Present in this are an A/T-rich box that binds MEF-2 protein(s) and G-rich (AGTGGGGGAGGGGGCTGCG) interacts with ubiquitously expressed factors. Both elements required for tissue-specific expression gene skeletal muscle cells. Here, we report identification characterization Kruppel-like zinc finger protein, termed repressor factor 1, sequence-specific manner to functions as transcription...
Laminar shear stress (LSS) represents a major athero-protective stimulus. However, the mechanisms for this effect are poorly characterized. As chemokine receptors modulate endothelial cell functions, we hypothesized that at least some LSS effects on cells (ECs) may be due to LSS-dependent changes in receptor expression and function. Exposure of Human umbilical vein (HUVECs) 15 dynes/cm2/sec(-1) strongly inhibited CXC 4 (CXCR4) transcriptional level impaired stromal-derived factor...
Abstract The stromal cell–derived factor (SDF)‐1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution postnatal myogenesis and the of alternate SDF‐1 receptor, CXC 7 (CXCR7), are poorly characterized. Western blot analysis real‐time polymerase chain reaction (PCR) were performed evaluate vitro effect CXCR4 CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, SDF‐1; during differentiation, levels...
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease in need of prognostic markers to address therapeutic choices. We have previously shown that alternative splicing the actin regulator, hMENA, generates hMENA11a, and hMENAΔv6 isoforms with opposite roles cell invasion. examined expression pattern hMENA by immunohistochemistry, using anti-pan specific anti-hMENA11a antibodies, 285 PDACs, 15 PanINs, 10 pancreatitis, normal pancreas. Pan immunostaining, absent pancreas low-grade...
Transforming growth factor beta (TGF) is a well-known inhibitor of myogenic differentiation as well an autocrine product rhabdomyosarcoma cells. We studied the role TGF-beta loop in regulating and human cell line, RD. previously reported that phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) induces arrest these cells, which constitutively express muscle regulatory factors. show TPA inhibits activation secreted latent TGF-beta, thus decreasing concentration active to cells are...
The chemokine receptor CXCR4 modulates endothelial progenitor cell migration, homing, and differentiation, plays a key role in cardiovascular regeneration. Here we examined the effect of ex vivo acidic preconditioning (AP) on expression regenerative potential mouse bone marrow (BM) ckit(+) cells.Acidic was achieved by exposing BM cells to hypercarbic acidosis (pH 7.0) for 24 h; control were kept at pH 7.4. Acidic enhanced stromal cell-derived factor 1 (SDF-1) mRNA levels, as well...
AimsAcidification is associated with a variety of pathological and physiological conditions. In the present study, we aimed at investigating whether acidic pH may regulate endothelial cell (EC) functions via chemokine receptor CXCR4, key modulator EC biological activities.
Enolase, a 46 kDa glycolytic enzyme, is an immunodominant antigen of Candida albicans , important human opportunistic pathogen. The full‐length coding sequence C. enolase gene was subcloned into the prokaryotic expression vector pDS56/RBSII, His 6 /E − under control inducible promoter to produce ‐tagged enolase. recombinant protein purified homogeneity by one‐step nickel‐chelate affinity chromatography. It recognized monoclonal antibody specific for enolase, as well anti‐enolase antibodies...
Nucleophosmin (NPM), a nucleolar multifunctional phosphoprotein, acts as stress sensor in different cell types. NPM can be actively secreted by inflammatory cells, however its biology on endothelium remains unexplored. In this study, we show for the first time that is human vein endothelial cells (HUVEC) early response to serum deprivation and pro-inflammatory angiogenic molecule both vitro vivo. Accordingly, 24 h of starvation condition induced relocalization from nucleus cytoplasm....
Rat alpha adducin point mutation (F316Y) has been associated with primary systemic arterial hypertension. As microcirculatory abnormalities are present in most forms of hypertension, the aim study was to investigate whether rat may regulate endothelial cell (EC) functions vitro and vivo.The overexpression wild type (WT-Add1) ECs induced capillary-like structure development Matrigel enhanced capillary formation implants vivo CD1 mice. In contrast, mutated form (MUT-Add1) had a Null effect...