A5103240427- MicroRNA in disease regulation
- Metabolism and Genetic Disorders
- Cancer-related molecular mechanisms research
- Folate and B Vitamins Research
- Circular RNAs in diseases
- Porphyrin Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Cancer, Hypoxia, and Metabolism
- Reproductive System and Pregnancy
- Angiogenesis and VEGF in Cancer
- Neonatal Health and Biochemistry
- Iron Metabolism and Disorders
- Pharmaceutical Practices and Patient Outcomes
- Aluminum toxicity and tolerance in plants and animals
- Cancer Research and Treatments
- Sirtuins and Resveratrol in Medicine
- Lysosomal Storage Disorders Research
- Trace Elements in Health
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Medication Adherence and Compliance
- Heavy Metal Exposure and Toxicity
- Cellular transport and secretion
- Frailty in Older Adults
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2015-2025
IRCCS Humanitas Research Hospital
2024
New York Medical College
2024
Azienda Ospedaliera Citta' della Salute e della Scienza di Torino
2023
Azienda Ospedaliera Universitaria Senese
2023
Policlinico S.Orsola-Malpighi
2023
Ospedale San Paolo
2023
IRCCS Ospedale San Raffaele
2023
Augusta University Health
2023
University of Milan
2015-2022
MicroRNAs (miRNAs) are small non-protein-coding RNAs that function as negative gene expression regulators. In the present study, we investigated miRNAs role in endothelial cell response to hypoxia. We found of miR-210 progressively increased upon exposure overexpression normoxic cells stimulated formation capillary-like structures on Matrigel and vascular growth factor-driven migration. Conversely, blockade via anti-miRNA transfection inhibited by hypoxia decreased migration factor. did not...
Aims: Reactive oxygen species (ROS) play a pivotal role in different pathologic conditions, including ischemia, diabetes, and aging. We previously showed that ROS enhance miR-200c expression, causing endothelial cell (EC) apoptosis senescence. Herein, we dissect the interaction among three strictly related proteins modulate EC function production: sirtuin 1 (SIRT1), nitric oxide synthase (eNOS), forkhead box O1 (FOXO1). Moreover, of on modulation was also investigated. Results: demonstrated...
MicroRNA-210 (miR-210) induction is a virtually constant feature of the hypoxic response in both normal and transformed cells, regulating several key aspects cardiovascular diseases cancer. We found that miR-210 was induced normoxic myoblasts upon myogenic differentiation vitro vivo. transcription activated an hypoxia-inducible factor 1-α (Hif1a)-dependent manner, chromatin immunoprecipitation experiments show Hif1a bound to promoter only differentiated myotubes. Accordingly, luciferase...
Proliferation of mammalian cardiomyocytes stops rapidly after birth and injured hearts do not regenerate adequately. High cyclin-dependent kinase inhibitor (CKI) levels have been observed in cardiomyocytes, but their role maintaining a post-mitotic state is still unknown. In this report, it was investigated whether CKI knockdown by RNA interference induced cardiomyocyte proliferation. We found that triple transfection with p21(Waf1), p27(Kip1), p57(Kip2) siRNAs both neonatal adult to enter S...
Acute intermittent porphyria (AIP) is a rare metabolic disorder characterized by acute attacks often triggered porphyrinogenic drugs and low-glucose diet. According to recent findings, chronic symptoms persist in AIP patients. To avoid the symptoms, patients adopt preventive strategies such as increasing glucose intake, suggesting that nutrition crucial aspect of disease management. Given strong connection between glucose, we assessed anthropometric data, biochemical data nutritional...
A close relationship exists between hyperglycaemia, oxidative stress, and diabetic complications. In fact, high glucose (HG) determines the overproduction of reactive oxygen species (ROS) by mitochondria. p66ShcA is a gene that regulates apoptotic responses to stress. Indeed, knockout (ko) mice display decreased ROS production increased resistance ROS-induced cell death in variety pathophysiological settings. Reduced endothelial progenitor (EPC) number, differentiation, function are relevant...
Peripheral artery disease is caused by the restriction or occlusion of arteries supplying leg. Better understanding molecular mechanisms underpinning tissue response to ischemia urgently needed improve therapeutic options. The aim this study investigate hypoxia-induced miR-210 regulation and its role in a mouse model hindlimb ischemia.miR-210 expression was induced femoral dissection. To miR-210, function inhibited systemic administration complementary locked nucleic acid...
Glucocorticoids epigenetically induce the expression of a stress hormone gene associated with labor in human placenta cells.
Abstract Purpose: Homeodomain-interacting protein kinase-2 (HIPK2), a corepressor for homeodomain transcription factors, is multifunctional kinase whose role in tumor cell survival not completely clarified. We addressed whether HIPK2 restrains colon tumorigenesis by turning off cytosolic phospholipase A2 (cPLA2)-dependent prostaglandin E2 (PGE2) generation the light of overwhelming evidence suggesting contribution this prostanoid variety cancers. Experimental Design: In human colorectal...
Wilson's disease (WD) is a rare autosomal-recessive disorder characterized by mutation in the ATP7B gene, located on chromosome 13, which encodes protein involved metabolism of copper.We described case an Indian male with history polydipsia and polyuria, related to hypercalciuria consequent nephrocalcinosis. The symptoms began at age five years old, but he was not diagnosed WD until reached adolescent age. We started therapy D-Penicillamine, B-vitamin complex recommended low copper diet....
Chronic obstructive pulmonary disease (COPD) and heart failure (HF) mutually increase the risk of being present in same patient, especially if older. Whether or not this coexistence may be associated with a worse prognosis is debated. Therefore, employing data derived from REPOSI register, we evaluated clinical features outcomes population elderly patients admitted to internal medicine wards having COPD, HF COPD + HF.We measured socio-demographic anthropometric characteristics, severity...
We address here the involvement of homeodomain‐interacting protein kinase 2 (HIPK2)/p53 complex on MDM2 regulation following apoptotic DNA damage. Our results provide a plausible transcriptional (p53‐dependent) and posttranscriptional (p53‐independent) double mechanism by which HIPK2 accomplishes downmodulation. First, in wtp53‐carrying cells HIPK2‐dependent p53Ser46 phosphorylation selectively inhibits at level. Secondly, interacts with vitro vivo promotes nuclear export proteasomal...
Abstract Aims The association between hyperglycemia at hospital admission and relevant short- long-term outcomes in elderly population is known. We assessed the effects on mortality of hyperglycemia, disability, multimorbidity internal medicine ward patients aged ≥ 65 years. Methods Data were collected from an active register 102 geriatric wards Italy (RePoSi project). Patients recruited during four index weeks a year. Socio-demographic data, reason for hospitalization, diagnoses, treatment,...
ABSTRACT In Pseudomonas fluorescens ST, the promoter of styrene catabolic operon, P styA , is induced by and repressed addition preferred carbon sources. regulated StyS/StyR two-component system. The integration host factor (IHF) also plays a positive role in regulation. Three distinct StyR binding sites, which have different affinities for this response regulator, been characterized on . high-affinity site (STY2) necessary activity. DNA region upstream STY2 contains lower-affinity site,...
Kawasaki disease is a multi-system vasculitis which usually occurs in children under 5 years of age. In infants three months age, it very rare and associated with high incidence incomplete or atypical forms, often unresponsive to treatment. This condition increases the risk cardiovascular complications such as coronary artery aneurysms.We describe 3-month-old infant who developed early severe aneurysms arteries despite timely administration intravenous immunoglobulins, followed by days...
Diabetes is an increasing global health burden with the highest prevalence (24.0%) observed in elderly people. Older diabetic adults have a greater risk of hospitalization and several geriatric syndromes than older nondiabetic adults. For these conditions, special care required prescribing therapies including anti- diabetes drugs. Aim this study was to evaluate appropriateness adherence safety recommendations prescriptions glucose-lowering drugs hospitalized patients diabetes. Data for...
Epilepsy is the most common chronic neurological disorder of dogs and requires a substantial commitment by pet owner. The aim this study was to evaluate how Italian owners epileptic receiving long-term treatment perceived their own quality life (QoL) that pet, using list key questions. A questionnaire sent affected recurrent seizures treated with antiepileptic drugs for at least three months. questions included signalment, medical history physical, social psychological aspects associated...
Problem A maternal serum biomarker profile analysis was performed to determine potential indicators of acute rejection pregnancy following in‐utero cell‐based treatments in pre‐immune embryos. Method study We used an established non‐human primate model for stem cell therapy at 38–42 days from fertilization. The concentrations nine candidate biomarkers were determined before and after the injection different cocktails human umbilical cord blood cells into gestational sac. All animals then...
Fabry disease is a rare X-linked lysosomal storage disorder caused by mutations in the GLA gene, leading to deficient α-galactosidase A activity and, consequently, glycosphingolipid accumulation wide variety of cells. due N215S (c.644A>G, p.Asn215Ser) missense mutation usually results late-onset phenotype presenting with isolated cardiac involvement. We herein present case patient involvement, namely left ventricular hypertrophy and arrhythmias, end-stage renal requiring kidney...