A5100760975- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Reproductive System and Pregnancy
- Preterm Birth and Chorioamnionitis
- Immune Cell Function and Interaction
- RNA Research and Splicing
- MicroRNA in disease regulation
- Pregnancy and preeclampsia studies
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Macrophage Migration Inhibitory Factor
- Histone Deacetylase Inhibitors Research
- Insect Resistance and Genetics
- Immune cells in cancer
- Pregnancy-related medical research
- Birth, Development, and Health
- Gut microbiota and health
- Maternal and fetal healthcare
- RNA Interference and Gene Delivery
- Stress Responses and Cortisol
- interferon and immune responses
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Growth Hormone and Insulin-like Growth Factors
Shanghai Tenth People's Hospital
2022-2024
Tongji University
2022-2024
Hubei University of Medicine
2024
Chongqing University
2024
University of Maryland, Baltimore
2021-2024
Zhejiang Normal University
2024
Kunming University
2024
Xuzhou Medical College
2024
Jilin University
2024
Capital Medical University
2011-2023
Background— Essential hypertension has been recognized as a disease resulting from combination of environmental and genetic factors. Recent studies demonstrated that microRNAs (miRNAs) are involved in cardiac hypertrophy heart failure. However, little is known about the roles miRNAs essential hypertension. Methods Results— Using microarray-based miRNA expression profiling, we compared expressions plasma samples 13 hypertensive patients 5 healthy control subjects. Twenty-seven were found to...
Abstract Alternative splicing enhances transcriptome diversity in all eukaryotes and plays a role plant tissue identity stress adaptation. To catalog new maize (Zea mays) transcripts identify genomic loci that regulate alternative splicing, we analyzed over 90 RNA-seq libraries from inbred lines B73 Mo17, as well Syn10 doubled haploid (progenies × Mo17). Transcript discovery was augmented with publicly available data 14 tissues, expanding the by more than 30,000 increasing percentage of...
MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate protein output from the majority of human mRNAs. In contrast to consensus view all miRNAs associated with Argonaute (Ago) proteins, we determine expressed in a 13-fold excess relative Agos HeLa cells and bound mRNAs sevenfold Agos, implying existence miRNA–mRNA duplexes not stoichiometrically by Agos. We show four can repress duplexes, but only Ago2 cleave interfering RNA–mRNA vitro. visualize direct Ago binding...
MicroRNAs (miRNAs) are small noncoding RNAs that may target more than one-third of human genes, yet the mechanisms used by miRNAs to repress translation mRNAs obscure. Using a recently described cell-free assay miRNA function, we observe miRNA-targeted enriched for 40S but not 60S ribosome components. Additionally, toeprinting analysis demonstrates approximately 18 nt 3' relative initiating AUG protected, consistent with subunits positioned at codon. Our results suggest initiation preventing...
Peptidoglycan recognition protein (PGRP) is an important pattern receptor in innate immunity that vital for bacterial and defense insects. Few studies report the role of PGRP viral infection. Here we cloned two forms from model lepidopteran Bombyx mori: BmPGRP2-1 a transmembrane protein, whereas BmPGRP2-2 intracellular protein. bound to DAP-type PGN activate canonical Imd pathway. knockdown reduced B. mori nucleopolyhedrovirus (BmNPV) multiplication mortality cell lines silkworm larvae,...
Abstract Although immunotherapies of tumors have demonstrated promise for altering the progression malignancies, been limited by an immunosuppressive tumor microenvironment (TME) that prevents infiltrating immune cells from performing their anticancer functions. Prominent among are myeloid-derived suppressor (MDSC) and tumor-associated macrophages (TAM) inhibit T via release cytokines engagement checkpoint receptors. Here, we explore properties MDSCs TAMs freshly isolated mouse human find...
Abstract The critical role of the tumor immune microenvironment (TIME) in determining response to checkpoint inhibitor (ICI) therapy underscores importance understanding cancer cell–intrinsic mechanisms driving immune-excluded (“cold”) TIMEs. One such cold is oral cavity squamous cell carcinoma (OSCC), a tobacco-associated with mutations TP53 gene which responds poorly ICI therapy. Because altered function promotes progression and plays potential TIME modulation, here we developed syngeneic...
Glucocorticoids epigenetically induce the expression of a stress hormone gene associated with labor in human placenta cells.
The role of the sputum microbiome in chronic obstructive pulmonary disease (COPD) progression remains elusive. As advent new culture-independent microbial sequencing technique makes it possible to disclose complex community respiratory tract. aim this study was use metagenomic next-generation (mNGS) confirm whether there are differences COPD between different exacerbation frequencies and lung function.Thirty-nine patients were divided into a frequent exacerbators (FE) group (n = 20)...
Placental CRH may be part of a clock that governs the length human gestation. The mechanism underlying differential regulation in placenta is poorly understood. We report here constitutively activated RelB/nuclear factor-κB2 (NF-κB)-2 (p100/p52) acts as an endogenous stimulatory signal to regulate by binding NF-κB enhancer gene promoter placenta. Nuclear staining NF-κB2 and RelB villous syncytiotrophoblasts cytotrophoblasts was coupled with cytoplasmic syncytial knots cytotrophoblasts....
Women exposed to phthalates during pregnancy are at increased risk for delivering preterm, but the mechanism behind this relationship is unknown. Placental corticotropin-releasing hormone (CRH) and cyclooxygenase-2 (COX-2) key mediators of parturition regulated by non-canonical NF-kB (RelB/p52) signaling pathway. In study, we demonstrate that one major phthalate metabolites, mono-(2-ethylhexyl)-phthalate (MEHP), CRH COX-2 mRNA protein abundance in a dose-dependent manner primary cultures...
Abstract Although the principle of systemic treatment to prevent progression oral premalignant lesions (OPL) has been demonstrated, there remains a lack consensus about an optimal approach that balances clinical efficacy with toxicity concerns. Recent advances in cancer therapy using approaches targeting tumor immune microenvironment (TIME) including immune-checkpoint inhibitors indicate these agents have significant clinically activity against different types cancers, cancer, and therefore...
Our recent study demonstrated that constitutively activated RelB/NF-κB2 positively regulates the CRH in human placenta. In current study, we explored role of glucocorticoid receptor (GR) signaling constitutive activation noncanonical NF-κB pathway. A response element (GRE) motif search suggests both inducing kinase (NIK) and RelB genes, which are key regulators pathway, have a putative GRE within their promoter, approximately 1 kb upstream from transcription start site. By using chromatin...