A5028498906- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cholesterol and Lipid Metabolism
- Lipid metabolism and biosynthesis
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Macrophage Migration Inhibitory Factor
- Immune cells in cancer
- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Bladder and Urothelial Cancer Treatments
- Platelet Disorders and Treatments
- Hormonal and reproductive studies
- Histone Deacetylase Inhibitors Research
- Steroid Chemistry and Biochemistry
- Cancer Research and Treatments
- Xenotransplantation and immune response
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- interferon and immune responses
Purdue University Institute for Cancer Research
2023-2024
Purdue University West Lafayette
2010-2023
Center for Cancer Research
2011-2017
State Street (United States)
2017
Purdue University System
2009
University of Iowa
1992-2008
Medical College of Wisconsin
2000-2004
University of Iowa Holden Comprehensive Cancer Center
2004
Tianjin Medical University
2004
Second Hospital of Tianjin Medical University
2004
Current paradigms suggest that, despite the heterogeneity of myeloid-derived suppressor cells (MDSC), all Gr-1(+) CD11b(+) can exert suppressive function when exposed to inflammatory stimuli. In vitro evaluation shows that MDSC from multiple tissue sites have activity, and in vivo inhibition enhances T-cell function; however, relative capacity present at localized or peripheral tissues suppress responses has not been directly evaluated. current study, we observed during a tissue-specific...
Abstract Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature that expand during benign and cancer-associated inflammation characterized by their ability to inhibit T cell immunity. Increased metabolism l-Arginine (l-Arg), through the enzymes arginase 1 NO synthase 2 (NOS2), is well documented as major MDSC suppressive mechanism. Therefore, we hypothesized restricting uptake l-Arg critical control point modulate activity. Using murine models prostate-specific...
Abstract Although immunotherapies of tumors have demonstrated promise for altering the progression malignancies, been limited by an immunosuppressive tumor microenvironment (TME) that prevents infiltrating immune cells from performing their anticancer functions. Prominent among are myeloid-derived suppressor (MDSC) and tumor-associated macrophages (TAM) inhibit T via release cytokines engagement checkpoint receptors. Here, we explore properties MDSCs TAMs freshly isolated mouse human find...
Corrosion of metal in biomedical devices could cause serious health problems to patients. Currently ceramics coating materials used implants can reduce corrosion some extent with limitations. Here we proposed graphene as a biocompatible protective film for potentially application. We confirmed effectively inhibits Cu surface from different biological aqueous environments. Results cell viability tests suggested that greatly eliminates the toxicity by inhibiting and reducing concentration Cu2+...
Abstract BACKGROUND Prostatitis is a poorly understood disease and increasing evidence suggests inflammation involved in other prostatic diseases including prostate cancer. METHODS The ability of pre‐activated CD8 T cells to induce prostatitis was examined by adoptive transfer antigen specific into POET‐3 mice or POET‐3/Luc/ Pten −/+ mice. Characterization the inflammatory response determined examining leukocyte infiltration histological analysis, flow cytometry evaluating cytokine chemokine...
Cholesterol accumulates in prostate lesions and has been linked to cancer incidence progression. However, how accumulated cholesterol contributes development progression is not completely understood. sulfate (CS), the primary sulfonation product of sulfotransferase (SULT2B1b), human adenocarcinoma precancerous prostatic intraepithelial neoplasia (PIN) compared with normal regions same tissue sample. Given enhanced accumulation CS these lesions, it was hypothesized that SULT2B1b-mediated...
Interleukin-1 (IL-1) is a growth arrest signal for diverse human tumor cell lines. We report here that the action of this cytokine in melanoma cells associated with induction EGR-1, zinc finger protein activates gene transcription. Both and EGR-1 are induced via type I receptor IL-1. To determine role IL-1 cells, we used chimera expressing transrepression domain Wilm's gene, WT1, DNA binding Egr-1. This competitively inhibited EGR-1-dependent transactivation GC-rich sequence, indicating it...
The expression of interleukin-6 (IL-6) by normal and malignant urothelium in response to bacillus Calmette-Guerin (BCG) may have direct indirect effects on the antitumor activity BCG. We evaluated molecular signaling pathway through which BCG induces IL-6 human transitional cell carcinoma lines.We messenger RNA protein lines Pharmacological inhibition synthesis was used determine if mediated induction occurred via an immediate-early or delayed pathway. 5' deletion analysis site directed...
BACKGROUND The presence of inflammation in prostate cancer (PCa) and benign hyperplasia (BPH) has been well described but the cellular mechanisms by which modulates are currently unclear. Prostate stem cells (PSC) not only maintain homeostasis also considered to be cell origin PCa an important contributor BPH. However, impact on PSC is understood. Therefore, we initiated studies evaluate effect PSC. METHOD Ovalbumin specific CD8+ T were intravenously delivered intact castrated ovalbumin...
Abstract The adjuvant therapy of choice for superficial bladder cancer is the intravesical instillation live Mycobacterium bovis bacillus Calmette‐Guerin (BCG). Despite fact that this most effective treatment cancer, administration BCG associated with high local morbidity and potential systemic infection. Therefore, there a need development safer, less toxic approaches to fight disease. Because fibronectin attachment protein (FAP) key element in retention targeting cells, we hypothesize can...
A synthetic peptidolipopolymer conjugate, incorporated into liposomes to promote specific binding the fibronectin (FBN) matrix surrounding bladder tumor cells and cellular internalization of FBN-integrin complexes, is reported. The peptide promotes association with MB49 mouse model in a sequence-specific concentration-dependent manner, maximum cell occurring at 2 mol % RWFV-PEG2000-DSPE. Double PEGylation liposome membrane (i.e., 4 mPEG1000-DSPE + RWFV-PEG2000-DSPE) enhanced by >1.6-fold,...
No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 May 2000AUTOCRINE IL-6 PRODUCTION BY HUMAN TRANSITIONAL CARCINOMA CELLS UPREGULATES EXPRESSION OF THE α5β1 FIBRONECTIN RECEPTOR GUANG-JIAN ZHANG, SCOTT A. CRIST, AMY K. MCKERROW, YONG XU, DAVID C. LADEHOFF, and WILLIAM SEE ZHANGGUANG-JIAN ZHANG , CRISTSCOTT CRIST MCKERROWAMY MCKERROW XUYONG XU LADEHOFFDAVID LADEHOFF SEEWILLIAM View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67678-1AboutFull TextPDF ToolsAdd to...
CD28 costimulation, an important second signal for antigen-mediated T cell activation, is known to enhance expression of several genes the regulation CD4+ effector function including interleukin-2 and CD154. Previous studies demonstrate CD28-mediated enhancement transcription Fas ligand (CD95L) in lines, suggesting a regulatory link between CD95L expression. These results served as basis structure/function analysis promoter elucidate mechanism CD95L. In this report, we describe novel...
Chronic prostate inflammation in patients with benign hyperplasia (BPH) correlates the severity of symptoms. How contributes to enlargement and/or BPH symptoms and underlying mechanisms remain unclear. In this study, we utilize a unique transgenic mouse model that mimics chronic non-bacterial prostatitis men investigate impact on androgen receptor (AR) basal stem cells (bPSC) their differentiation vivo. We find significantly enhances AR levels activity bPSC. More importantly, identify...
Studies have demonstrated elevated expression and secretion of IL-6 by transitional cell carcinomas (TCC) following bacillus Calmette-Guerin (BCG) therapy. At present, the role on biology TCC is poorly understood. This study evaluated influence a critical variable regulating BCG-tumor interaction, tumor alpha5beta1 integrin.A human line (253J) was transfected with an vector containing full-length cDNA sequence. Overexpression mRNA protein confirmed Northern analysis ELISA, respectively....