A5038494195- Mass Spectrometry Techniques and Applications
- Thyroid Cancer Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Advanced Chemical Sensor Technologies
- Gene expression and cancer classification
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- AI in cancer detection
- Cholesterol and Lipid Metabolism
- Ovarian cancer diagnosis and treatment
- Radiomics and Machine Learning in Medical Imaging
- Analytical chemistry methods development
- Biosensors and Analytical Detection
- Hormonal and reproductive studies
- Research Data Management Practices
- Breast Lesions and Carcinomas
- Metabolism and Genetic Disorders
- Forensic Fingerprint Detection Methods
Baylor College of Medicine
2022-2025
The University of Texas at Austin
2015-2023
United States Department of Homeland Security
2021
Stanford University
2013-2017
Purdue University West Lafayette
2007-2016
Universidade Estadual de Campinas (UNICAMP)
2006-2016
Brigham and Women's Hospital
2011
Harvard University
2011
Dana-Farber Cancer Institute
2011
Center for Cancer Research
2010
A handheld and biocompatible mass spectrometry system provides nondestructive molecular diagnosis of cancer tissue samples.
Brain tissue biopsies are required to histologically diagnose brain tumors, but current approaches limited by characterization at the time of surgery. Emerging technologies such as mass spectrometry imaging can enable a rapid direct analysis cancerous based on molecular composition. Here, we illustrate how gliomas be rapidly classified desorption electrospray ionization-mass (DESI-MS) imaging, multivariate statistical analysis, and machine learning. DESI-MS was carried out 36 human glioma...
The main goal of brain tumor surgery is to maximize resection while preserving function. However, existing imaging and surgical techniques do not offer the molecular information needed delineate boundaries. We have developed a system rapidly analyze classify tumors based on lipid acquired by desorption electrospray ionization mass spectrometry (DESI-MS). In this study, classifier was built discriminate gliomas meningiomas 36 glioma 19 meningioma samples. tested results were validated for...
Significance The diagnosis of tumors during surgery still relies principally on an approach developed over 150 y ago: frozen section microscopy. We show that a validated molecular marker—2-hydroxyglutarate generated from isocitrate dehydrogenase 1 mutant gliomas—can be rapidly detected using form ambient MS does not require sample preparation. use the Advanced Multimodality Image Guided Operating Suite at Brigham and Women’s Hospital to demonstrate desorption electrospray ionization could...
Abstract Cardinal is an R package for statistical analysis of mass spectrometry-based imaging (MSI) experiments biological samples such as tissues. supports both Matrix-Assisted Laser Desorption/Ionization (MALDI) and Desorption Electrospray Ionization-based MSI workflows, with multiple tissues complex designs. The main analytical functionalities include (1) image segmentation, which partitions a tissue into regions homogeneous chemical composition, selects the number segments subset...
The MYC oncogene is frequently mutated and overexpressed in human renal cell carcinoma (RCC). However, there have been no studies on the causative role of or any other initiation maintenance kidney tumorigenesis. Here, we show through a conditional transgenic mouse model that oncogene, but not RAS initiates maintains RCC. Desorption electrospray ionization-mass-spectrometric imaging was used to obtain chemical maps metabolites lipids RCC samples. Gene expression analysis revealed tumors...
Significance Complete resection of a tumor is associated with an improved prognosis for most types solid malignancies. In gastric-cancer surgery, surgical-margin evaluation commonly performed intraoperatively by histopathologic frozen sections. However, frozen-section results are subjective and can be unreliable in up to 30% patients undergoing gastrointestinal cancers. We used desorption electrospray ionization mass spectrometric imaging (DESI-MSI) the statistical method least absolute...
The standard treatment for high-grade serous ovarian cancer is primary debulking surgery followed by chemotherapy. extent of metastasis and invasive potential lesions can influence the outcome these surgeries. Here, we explored underlying mechanisms that could increase metastatic in cancer. We discovered FABP4 (fatty acid binding protein) substantially cells. also found miR-409-3p regulates cells hypoxia decreases levels. Treatment with DOPC nanoliposomes containing either mimic or siRNA...
Desorption electrospray ionization mass spectrometry (DESI-MS) is employed in the forensic analysis of documents. Blue ballpoint pen inks applied to ordinary writing paper are examined under ambient conditions without any prior sample preparation. When coupled an automated moving stage, two-dimensional molecular images generated. Proof-of-principle experiments include characterization a simulated forged number and examination older written records. This application DESI has advantages over...
Development of methods for rapid distinction between cancerous and non-neoplastic tissues is an important goal in disease diagnosis. To this end, desorption electrospray ionization mass spectrometry (DESI-MS) imaging was applied to analyze the lipid profiles thin tissue sections 68 samples human prostate cancer normal tissue. The state determined by independent histopathological examination. Cholesterol sulfate identified as a differentiating compound, found almost exclusively including...
Imaging, not imagination: Three-dimensional (3D) images can be constructed from a set of 2D data acquired by desorption electrospray ionization (DESI) mass spectrometry. The 3D show the spatial distributions specific biomolecules in substructures mouse brain (see picture). Two-dimensional (2D) imaging spectrometry (MS)1 has emerged as powerful technique biological sciences. It allows direct investigation distribution variety lipids, drugs, defensive agents, pigments, and proteins plant...
Differentiation of human brain astrocytic tumor grades can be achieved by direct lipid analysis using desorption electrospray ionization mass spectrometry (DESI-MS). Distinctive profiles are associated with the degree malignancy, II, III, and IV (see picture). Detailed facts importance to specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted authors. Please note: The publisher is responsible for...
Maintaining your integrity: Morphologically friendly DESI-MS imaging of tissue can be performed prior to histochemical and immuno-histochemical analysis on same section by using new solvent systems. This novel capability allows chemical information obtained while preserving integrity. Imaging mass spectrometry (MS) is a powerful technique that has been gaining increased attention broad use in many fields science. Many biological applications are being pursued make the comprehensive it...
Significance We studied lung tumors induced by oncogene KRAS gene mutation using transgenic mice and human specimens. Gene expression analyses show that induces fatty acid synthase (FASN), promoting lipogenesis. Through desorption electrospray ionization MS imaging, we found specific lipid modifications in adenocarcinoma. Nanoimmunoassay identified KRAS-associated phosphoprotein signatures. showed activates the ERK2 protein, whereas non-KRAS adenocarcinoma shows elevated ERK1. inhibited FASN...
Overexpression of the v-myc avian myelocytomatosis viral oncogene homolog (MYC) is one most commonly implicated causes human tumorigenesis. MYC known to regulate many aspects cellular biology including glucose and glutamine metabolism. Little about relationship between appearance disappearance specific lipid species. We use desorption electrospray ionization mass spectrometry imaging (DESI-MSI), statistical analysis, conditional transgenic animal models cell samples investigate changes in...
Analysis of large biomolecules including proteins has proven challenging using ambient ionization mass spectrometry imaging techniques. Here, we have successfully optimized desorption electrospray (DESI-MS) to detect intact directly from tissue sections and further integrated DESI-MS a high field asymmetric waveform ion mobility (FAIMS) device for protein imaging. Optimized DESI-FAIMS-MS parameters were used image mouse kidney, brain, human ovarian breast samples, allowing detection 11, 16,...
Abstract Diagnosis of human bladder cancer in untreated tissue sections is achieved by using imaging data from desorption electrospray ionization mass spectrometry (DESI‐MS) combined with multivariate statistical analysis. We use the distinctive DESI‐MS glycerophospholipid (GP) spectral profiles to visually characterize and formally classify twenty pairs (40 samples) cancerous adjacent normal samples. The individual ion images derived acquired correlate standard histological hematoxylin...