A5103222728- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Molecular Biology Techniques and Applications
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- SARS-CoV-2 detection and testing
- Ion-surface interactions and analysis
- Monoclonal and Polyclonal Antibodies Research
- Thyroid Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Medical Imaging and Pathology Studies
- Advanced X-ray and CT Imaging
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Sensors
- Pancreatic and Hepatic Oncology Research
- 3D Printing in Biomedical Research
- Thyroid and Parathyroid Surgery
- Radiomics and Machine Learning in Medical Imaging
- Parathyroid Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Capillary Electrophoresis Applications
The University of Texas at Austin
2017-2024
Merck & Co., Inc., Rahway, NJ, USA (United States)
2020
A handheld and biocompatible mass spectrometry system provides nondestructive molecular diagnosis of cancer tissue samples.
Intraoperative delineation of tumor margins is critical for effective pancreatic cancer surgery. Yet, intraoperative frozen section analysis a time-consuming and often challenging procedure that can yield confounding results due to histologic heterogeneity tissue-processing artifacts. We have previously described the development MasSpec Pen technology as handheld mass spectrometry-based device nondestructive tissue analysis. Here, we evaluated usefulness diagnosis ductal adenocarcinoma based...
Importance Surgery with complete tumor resection remains the main treatment option for patients breast cancer. Yet, current technologies are limited in providing accurate assessment of tissue vivo, warranting development new surgical guidance. Objective To evaluate performance MasSpec Pen intraoperative tissues and margins based on metabolic lipid information. Design, Setting, Participants In this diagnostic study conducted between February 23, 2017, August 19, 2021, mass spectrometry–based...
Intraoperative identification of tissues through gross inspection during thyroid and parathyroid surgery is challenging yet essential for preserving healthy tissue improving outcomes patients.
Distinguishing adenocarcinoma and squamous cell carcinoma subtypes of non-small lung cancers is critical to patient care. Preoperative minimally-invasive biopsy techniques, such as fine needle aspiration (FNA), are increasingly used for cancer diagnosis subtyping. Yet, histologic distinction in FNA material can be challenging. Here, we evaluated the usefulness desorption electrospray ionization mass spectrometry imaging (DESI-MSI) diagnose differentiate tissues samples.DESI-MSI was analyze...
Intraoperative tissue analysis and identification are critical to guide surgical procedures improve patient outcomes. Here, we describe the clinical translation evaluation of MasSpec Pen technology for molecular in vivo freshly excised tissues operating room (OR).An Orbitrap mass spectrometer equipped with a interface was installed an OR. A "dual-path" designed programmed studies 2 parallel systems that facilitated operation Pen. The devices were autoclaved before each procedure used by...
The outbreak of COVID-19 has created an unprecedent global crisis. While the polymerase chain reaction (PCR) is gold standard method for detecting active SARS-CoV-2 infection, alternative high-throughput diagnostic tests are a significant value to meet universal testing demands. Here, we describe new design MasSpec Pen technology integrated electrospray ionization (ESI) direct analysis clinical swabs and investigate its use screening. redesigned system incorporates disposable sampling device...
In this study, we used multiple enzyme digestions, coupled with higher-energy collisional dissociation (HCD) and electron-transfer/higher-energy collision (EThcD) fragmentation to develop a mass-spectrometric (MS) method for determining the complete protein sequence of monoclonal antibodies (mAbs). The was refined on an mAb known sequence, SARS-CoV-1 antireceptor binding domain (RBD) spike antibody. data were searched using Supernovo generate template-assisted de novo two SARS-CoV-2 mAbs...
Intraoperative tissue analysis is critical to guide surgical procedures and improve patient outcomes. Here, we describe the clinical translation intraoperative use of MasSpec Pen technology for direct molecular in vivo freshly excised tissues operating room. In this study, was used by surgeons staff during 100 surgeries over a 12-month period, allowing rapid detection rich mass spectral profiles from 715 ex analyses performed on thyroid, parathyroid, lymph node, breast, pancreatic, bile duct...
Abstract The outbreak of COVID-19 has created an unprecedent global crisis. While PCR is the gold standard method for detecting active SARS-CoV-2 infection, alternative high-throughput diagnostic tests are significant value to meet universal testing demands. Here, we describe a new design MasSpec Pen technology integrated electrospray ionization (ESI) direct analysis clinical swabs and investigate its use screening. redesigned system incorporates disposable sampling device refined uniform...
Abstract Precise removal of pancreatic ductal adenocarcinoma (PDAC) with microscopically negative margins, commonly assessed by frozen section analysis, is associated longer disease-free survival. However, histologic complexities and tissue-processing artifacts can render analysis PDAC margins a challenging time-consuming task, reported accuracies dependent on the skillset subspecialty pathologist call. We developed MasSpec Pen, handheld device coupled to mass spectrometer, for rapid (~15 s)...