A5047616959- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Gene expression and cancer classification
- AI in cancer detection
- Metabolomics and Mass Spectrometry Studies
- Radiomics and Machine Learning in Medical Imaging
- Advanced Proteomics Techniques and Applications
- Ovarian cancer diagnosis and treatment
- Advanced Chemical Sensor Technologies
- Research Data Management Practices
- Breast Lesions and Carcinomas
- Ion-surface interactions and analysis
- Various Chemistry Research Topics
- Analytical Chemistry and Chromatography
- Advanced X-ray and CT Imaging
- thermodynamics and calorimetric analyses
- Geographic Information Systems Studies
- Analytical chemistry methods development
- Cancer, Lipids, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- Scientific Computing and Data Management
- Microfluidic and Capillary Electrophoresis Applications
- Dendrimers and Hyperbranched Polymers
- Analytical Chemistry and Sensors
The University of Texas at Austin
2016-2024
Thermo Fisher Scientific (United States)
2021
University of San Francisco
2016
Analysis of large biomolecules including proteins has proven challenging using ambient ionization mass spectrometry imaging techniques. Here, we have successfully optimized desorption electrospray (DESI-MS) to detect intact directly from tissue sections and further integrated DESI-MS a high field asymmetric waveform ion mobility (FAIMS) device for protein imaging. Optimized DESI-FAIMS-MS parameters were used image mouse kidney, brain, human ovarian breast samples, allowing detection 11, 16,...
Ambient ionization mass spectrometry imaging (MSI) has been increasingly used to investigate the molecular distribution of biological tissue samples. Here, we report integration and optimization desorption electrospray (DESI) liquid-microjunction surface sampling probe (LMJ-SSP) with a chip-based high-field asymmetric waveform ion mobility (FAIMS) device image metabolites, lipids, proteins in Optimized FAIMS parameters for specific classes enabled semitargeted detection multiply charged...
Abstract BACKGROUND Accurate tissue diagnosis during ovarian cancer surgery is critical to maximize excision and define treatment options. Yet, current methods for intraoperative evaluation can be time intensive subjective. We have developed a handheld biocompatible device coupled mass spectrometer, the MasSpec Pen, which uses discrete water droplet molecular extraction rapid diagnosis. Here we evaluated performance of this technology across different sample sets, types, spectrometry...
Minimally invasive robotic-assisted surgeries have been increasingly used as a first-line of treatment for patients undergoing oncologic surgeries. In-situ tissue identification is critical to guide resection and assist decision-making. Traditional intraoperative histopathologic analysis frozen sections can be time-consuming present logistical challenges which interrupt surgical workflows. We report the development implementation laparoscopic, drop-in version MasSpec Pen device integrated...
Intraoperative delineation of tumor margins is critical for effective pancreatic cancer surgery. Yet, intraoperative frozen section analysis a time-consuming and often challenging procedure that can yield confounding results due to histologic heterogeneity tissue-processing artifacts. We have previously described the development MasSpec Pen technology as handheld mass spectrometry-based device nondestructive tissue analysis. Here, we evaluated usefulness diagnosis ductal adenocarcinoma based...
Importance Surgery with complete tumor resection remains the main treatment option for patients breast cancer. Yet, current technologies are limited in providing accurate assessment of tissue vivo, warranting development new surgical guidance. Objective To evaluate performance MasSpec Pen intraoperative tissues and margins based on metabolic lipid information. Design, Setting, Participants In this diagnostic study conducted between February 23, 2017, August 19, 2021, mass spectrometry–based...
Ambient ionization mass spectrometry has been widely applied to image lipids and metabolites in primary cancer tissues with the purpose of detecting understanding metabolic changes associated development progression. Here, we report use desorption electrospray (DESI-MS) metastatic breast thyroid human lymph node tissues. Our results show clear alterations lipid metabolite distributions detected spectra profiles from 42 samples tumors, normal 2D DESI-MS ion images selected molecular species...
Free fatty acids (FA) are a vital component of cells and critical to cellular structure function, so much that alterations in FA often associated with cell malfunction disease. Analysis from biological samples can be achieved by mass spectrometry (MS), but these analyses not capable distinguishing the fine structural within isomers limited global profiling lipids without spatial resolution. Here, we present use ultraviolet photodissociation (UVPD) for characterization double bond positional...
Intraoperative identification of tissues through gross inspection during thyroid and parathyroid surgery is challenging yet essential for preserving healthy tissue improving outcomes patients.
Developing alternative MS/MS strategies to distinguish isomeric lipids has become a high impact goal in shotgun lipidomics. Novel approaches have been developed resolve structural features that are not discernible by traditional methods and consequently promoted the discovery of new disease biomarkers. However, these largely limited characterizing with low complexity. Here, ultraviolet photodissociation (UVPD) for phospholipid characterization expanded analysis cardiolipins (CL), class...
Structural characterization of glycerophospholipids beyond the fatty acid level has become a major endeavor in lipidomics, presenting an opportunity to advance understanding intricate relationship between lipid metabolism and disease state. Distinguishing subtle structural features, however, remains challenge for high-throughput workflows that implement traditional tandem mass spectrometry (MS/MS) techniques, stunting molecular depth quantitative strategies. Here, reversed phase liquid...
Abstract Endometriosis is a pathologic condition affecting approximately 10% of women in their reproductive years. Characterized by abnormal growth uterine endometrial tissue other body areas, endometriosis can cause severe abdominal pain and/or infertility. Despite devastating consequences to patients’ quality life, the causes are not fully understood and validated diagnostic markers for have been identified. Molecular analyses ectopic eutopic tissues could lead enhanced understanding...
Intraoperative tissue analysis and identification are critical to guide surgical procedures improve patient outcomes. Here, we describe the clinical translation evaluation of MasSpec Pen technology for molecular in vivo freshly excised tissues operating room (OR).An Orbitrap mass spectrometer equipped with a interface was installed an OR. A "dual-path" designed programmed studies 2 parallel systems that facilitated operation Pen. The devices were autoclaved before each procedure used by...
Analytical methods that allow rapid, sensitive, and specific chemical measurements are central to forensic analysis essential accelerating compound screening confirmation. We have previously reported the development of MasSpec Pen technology as an easy-to-use disposable hand-held device integrated a mass spectrometer for direct molecular profiling biological samples. In this Technical Note, we describe new apparatus integrates with subatmospheric pressure ionization (sub-APCI) source ion...
Ambient ionization mass spectrometry (MS) techniques, such as desorption electrospray (DESI), have been increasingly used due to their simplicity, minimal sample preparation requirements, and potential applications in the field clinic. However, intrinsic nature, performance of these methods is susceptible variations ambient conditions. Here, we present data that suggests DESI-MS analysis becomes inconsistent below a relative humidity (RH) level ~ 35%. At low RH, hypothesize DESI spray...
Intraoperative tissue analysis is critical to guide surgical procedures and improve patient outcomes. Here, we describe the clinical translation intraoperative use of MasSpec Pen technology for direct molecular in vivo freshly excised tissues operating room. In this study, was used by surgeons staff during 100 surgeries over a 12-month period, allowing rapid detection rich mass spectral profiles from 715 ex analyses performed on thyroid, parathyroid, lymph node, breast, pancreatic, bile duct...