Significance Ewing sarcoma is a pediatric bone malignancy driven by the fusion protein EWS/FLI. EWS/FLI mediates oncogenesis through its role as an aberrant transcription factor, but little known about molecular mechanisms underlying this function. We demonstrate in cells that activates gene targets binding at associated GGAA-microsatellites, and these repetitive sequences are necessary for cell proliferation anchorage-independent growth. Furthermore, we show previously unknown EWS portion...

Minimally invasive robotic-assisted surgeries have been increasingly used as a first-line of treatment for patients undergoing oncologic surgeries. In-situ tissue identification is critical to guide resection and assist decision-making. Traditional intraoperative histopathologic analysis frozen sections can be time-consuming present logistical challenges which interrupt surgical workflows. We report the development implementation laparoscopic, drop-in version MasSpec Pen device integrated...

A new closure technique is introduced, which uses strips of macroporous polypropylene mesh as a suture for abdominal wall defects due to failures standard sutures and difficulties with planar meshes.Strips 2 cm width were passed through the tied simple interrupted sutures. The surgical outcomes are presented.One hundred seven patients underwent sutured closure. Seventy-six had preoperative hernias, mean hernia by CT scan those scans was 9.1 cm. Forty-nine fields clean-contaminated,...

EWS/FLI is the pathognomic fusion oncoprotein that drives Ewing sarcoma. The amino-terminal EWS portion coordinates transcriptional regulation and carboxy-terminal FLI contains an ETS DNA-binding domain. acts as aberrant transcription factor, orchestrating a complex mix of gene activation repression, from both high affinity motifs repetitive GGAA-microsatellites. Our overarching hypothesis executing multi-faceted requires to use distinct molecular mechanisms at different loci. Many attempts...

Many cancers are characterized by chromosomal translocations which result in the expression of oncogenic fusion transcription factors. Typically, these proteins contain an intrinsically disordered domain (IDD) fused with DNA-binding (DBD) another protein and orchestrate widespread transcriptional changes to promote malignancy. These fusions often sole recurring genomic aberration they cause, making them attractive therapeutic targets. However, targeting factors requires a better...

Paediatric cancers commonly harbour quiet mutational landscapes and are instead characterized by single driver events such as the mutation of critical chromatin regulators, expression oncohistones, or oncogenic fusion proteins. These ultimately promote malignancy through disruption normal gene regulation development. The protein in Ewing sarcoma, EWS/FLI, is an transcription factor that reshapes enhancer landscape, resulting widespread transcriptional dysregulation. Lysine-specific...

The role of cutaneous viral infections in the development non-melanoma skin cancer (NMSC), including squamous cell carcinoma (SCC), among chronic lymphocytic leukemia (CLL) and blood marrow transplant (BMT) patients is not established. CLL (n = 977) BMT 3587) treated at Moffitt Cancer Center were included a retrospective cohort study. Human papillomavirus (HPV) human polyomavirus (HPyV) DNA examined subset incident SCC tumors. Five-year cumulative incidence NMSC was 1.42% both 31 NMSCs) 18...

Lynch syndrome (LS) is the most common hereditary colorectal cancer syndrome, caused by germline mutations in mismatch-repair genes. Besides a lifetime risk of averaging 70%-80%, there an increased extracolonic tumors including gastric cancer. The utility screening gastroscopy has long been debated. This study aimed to determine proportion abnormal gastroscopies among patients screened, incidence and prevalence precursor lesions.Charts with mutation-proven between January 1, 2004, December...

Aging-related bone loss significantly impacts the growing elderly population globally, leading to debilitating conditions such as osteoporosis. Senescent osteocytes play a crucial role in aging process of bone. This longitudinal study examines impact continuous local and paracrine exposure senescence-associated secretory phenotype (SASP) factors on biophysical biomolecular markers osteocytes. We found significant cytoskeletal stiffening irradiated osteocytes, accompanied by expansion F-actin...

Atherosclerotic innominate artery occlusive disease can lead to cerebral and upper extremity ischemia. Innominate angioplasty stenting be complicated by stent fractures restenosis; furthermore, this technique is limited in treatment of occlusions. Ministernotomy the second or third intercostal space used instead conventional full sternotomy for open surgical revascularization with excellent perioperative long-term outcomes. This series three consecutive patients highlights aorta-innominate...

ABSTRACT Pediatric cancers commonly harbor quiet mutational landscapes and are instead characterized by single driver events such as the mutation of critical chromatin regulators, expression oncohistones, or oncogenic fusion proteins. These ultimately promote malignancy through disruption normal gene regulation development. The protein in Ewing sarcoma, EWS/FLI, is an transcription factor that reshapes enhancer landscape, resulting widespread transcriptional dysregulation. Lysine-specific...

Background: Metabolic syndrome includes a plethora of symptoms ultimately leading to obesity and type 2 diabetes. Obesity has been linked chronic gastrointestinal dysfunction (constipation, or diarrhea) specifically females with high BMI have associated increased prevalence diarrhea. We aimed better understand the influence fat-high sugar diet (Western diet) on small intestinal function assess sex-dependent effects. Methods: measured transepithelial short circuit current (Isc) measure...

Background/Aims: The goal of this study was to determine the influence high-fat high-sugar diet (Western diet) on intestinal function and subsequently if there were any beneficial effects exercise, genistein (a naturally occurring phytoestrogen) or both, intestine. Methods: We measured transepithelial short circuit current (Isc), across freshly isolated segments jejunum from male female C57Bl/6J mice randomly assigned one following groups for 12-week duration: (HFS), HFS with (Gen), exercise...

Abstract Objective: This study aims to define the biochemical characteristics of EWS required for distinct modes EWS/FLI-mediated gene regulation. EWS/FLI is fusion protein and oncogenic transcription factor that drives Ewing sarcoma. While well characterized as a transcriptional activator, both activation repression are necessary oncogenesis. Whether active or passive unknown. We previously demonstrated recruitment nucleosome modeling deacetylase (NuRD) lysine specific demethylase 1 (LSD1)...

Chronic consumption of a western diet (high fat with high sugar, HFHS) is associated type 2 diabetes, inflammation. Genistein naturally occurring isoflavone known to exert anti‐inflammatory properties and improve insulin sensitivity. Similar benefits have also been moderate exercise. This study aimed determine whether dietary genistein (600 mg genistein/kg diet, Gen) or exercise (Ex), both (Gen+Ex) would reduce the obese‐diabetic phenotype thus mitigate intestinal dysfunction induced by...

Many cancers are characterized by chromosomal translocations which result in the expression of oncogenic fusion transcription factors. Typically, these proteins contain an intrinsically disordered domain (IDD) fused with DNA-binding (DBD) another protein and orchestrate widespread transcriptional changes to promote malignancy. These fusions often sole recurring genomic aberration they cause, making them attractive therapeutic targets. However, targeting factors requires a better...