A5015970126- Diet and metabolism studies
- Phytoestrogen effects and research
- Diet, Metabolism, and Disease
- Digestive system and related health
- Cystic Fibrosis Research Advances
- Nutrition, Genetics, and Disease
- Gut microbiota and health
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Helicobacter pylori-related gastroenterology studies
- Adipose Tissue and Metabolism
- Osteoarthritis Treatment and Mechanisms
- Neonatal Respiratory Health Research
- Ion Transport and Channel Regulation
- Asthma and respiratory diseases
- Birth, Development, and Health
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- Bone Metabolism and Diseases
- Nutritional Studies and Diet
- Fatty Acid Research and Health
- Liver Disease Diagnosis and Treatment
- Ultrasound in Clinical Applications
- Infant Nutrition and Health
- Gastrointestinal motility and disorders
Midwestern University
2016-2025
Cellular Research (United States)
2016
University of Missouri
1999-2002
Case Western Reserve University
1996-1997
To date, there is no cure or effective treatment for Alzheimer’s disease (AD), a chronic neurodegenerative condition that affects memory, language, and behavior. AD characterized by neuroinflammation, accumulation of brain amyloid-beta (Aβ) oligomers neurofibrillary tangles, increased neuronal apoptosis, loss synaptic function. Promoting regular exercise diet containing polyphenols are non-pharmacological approaches prevent the progression diseases. In this study, we measured various...
Diabetes mellitus (DM)-induced brain damage is characterized by cellular, molecular and functional changes. The mechanisms include oxidative stress, neuroinflammation, reduction of neurotrophic factors, insulin resistance, excessive amyloid beta (Aβ) deposition Tau phosphorylation. Both antidiabetic neuroprotective effects the phytoestrogen genistein have been reported. However, beneficial effect in ob/ob mouse model severe obesity diabetes remains to be determined.In this study, female mice...
Abstract Context Point-of-care ultrasound (POCUS) has diverse applications across various clinical specialties, serving as an adjunct to findings and a tool for increasing the quality of patient care. Owing its multifunctionality, growing number medical schools are increasingly incorporating POCUS training into their curriculum, some offering hands-on during first 2 years didactics others utilizing longitudinal exposure model integrated all 4 school education. Midwestern University Arizona...
Chloride channel activity of cystic fibrosis transmembrane conductance regulator (CFTR) requires activation protein kinase A (PKA) by 3'-5'-cyclic adenosine monophosphate (cAMP). The level cAMP is controlled the balance between synthesis and hydrolysis adenylate cyclase phosphodiesterases (PDEs), respectively. CFTR appears to be most sensitive type III cyclic nucleotide PDEs in Calu-3 16HBE cells, both derived from airway epithelium expressing wild-type CFTR. Type can identified their...
We tested the effect of exercise training and genistein treatment on splenomegaly in mice fed a high-fat, high-sugar diet (HFSD).Male female C57BL6 HFSD containing 60% fat along with drinking water 42 g/L sugar (55% sucrose/45% fructose) for 12 weeks exhibited significant obesity, hyperglycemia, elevated plasma IL-6 levels. This was accompanied by characterized spleen weights 50% larger than standard chow (P < 0.05) enlarged rad white pulps. Mice treated combination (30 min/day, 5 days/week)...
We have investigated the mechanism of action two benzimidazolone analogs (NS004 and NS1619) on DeltaF508-CFTR using both whole-cell cell-attached patch-clamp techniques compared their effects with those genistein. conclude that genistein act through a common mechanism, based following evidence: 1) only phosphorylated CFTR, 2) maximal current activated by is identical to induced genistein, 3) increase open probability forskolin-dependent channel activity an time decrease closed (effects...
Abstract Energy‐dense foods can alter gut microbial diversity. However, the physiological effects of diet‐induced changes on development nonalcoholic fatty liver disease (NAFLD) remain debatable. We hypothesized that high‐fat intake for 6 weeks would promote intestinal dysbiosis by increasing gram‐positive bacteria, inducing production proinflammatory cytokines and subsequent hepatic lipid infiltration in young male rats. Six‐week old Sprague–Dawley rats were divided into two groups fed...
Skeletal muscle is composed of bundles fibers with distinctive characteristics. Oxidative fiber types contain higher mitochondrial content, relying primarily on oxidative phosphorylation for ATP generation. Notably, as a result obesity, or following prolonged exposure to high-fat diet, skeletal undergoes shift in type toward glycolytic type. Mitochondria are highly dynamic organelles, constantly undergoing biogenesis and processes. Our study aims explore the impact obesity dynamics also...
The cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel bears two nucleotide‐binding domains (NBD1 and NBD2) that control its ATP‐dependent gating. Exactly how these NBDs gating is controversial. To address this issue, we examined channels with a Walker‐A lysine mutation in NBD1 (K464 A) using the patch clamp technique. K464A mutants have an ATP dependence (EC 50 ≈ 60 μ m ) opening rate at 2.75 (∼ 2.1 s −1 similar to wild type 97 ; ∼ 2.0 ). However, K464A's closing...
Obese, type two diabetics are at an increased risk for fracturing their limb bones in comparison to the general population. Phytoestrogens like as soy isoflavone genistein have been shown protect against bone loss. In this study, we tested effects of treatment on femurs ob/ob mice, a model obesity and diabetes mellitus.Twenty six-week-old female mice were divided into obese (ob/ob) control, genistein-treated, lean (ob/+) genistein-treated groups (n = 5 each). Treatment with consisted 600 mg...
Many heterologously expressed mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) exhibit residual chloride channel activity that can be stimulated by agonists adenylate cyclase/protein kinase A pathway. Because clinical implications for activating in vivo, we are investigating whether deltaF508, most common disease-associated CFTR mutation, activated airway epithelial cells. We have found that, 36Cl- efflux 19-61% above baseline beta-adrenoreceptor and...
Diabetic obesity in the leptin-deficient ob/ob mouse is associated with weight gain, and hyperglycemia, along hyperinsulinemia. We have previously examined effects of genistein (a naturally occurring isoflavone found soy) on metabolic disturbances demonstrated beneficial (600 mg genistein/kg diet, for 4-weeks) T3 production corticosterone status. The goal this study was to examine whether dietary could prevent, or at least lessen, typical phenotype murine model diabetic-obesity, assess...
Avian ceca, a pair of blind sacs arising from the junction ileum and colon, are homologous to cecum in mammals. Cecal size is hypothesized depend on dietary proclivities pressures, with faunivorous species having short whereas herbivorous have long ceca. Previous tests this hypothesis, however, did not account for phylogenetic pseudoreplication among closely related taxa. We collated published data cecal length, category, flying ability, body mass 155 avian Character states were mapped onto...
The prevalence of obesity and related consequences, including insulin resistance Alzheimer's-like neuropathology, has increased dramatically. Contributing to this is the shift in lifestyle preference away from wholesome foods exercise Western-style diet sedentarism. Despite advances drug development, a healthy regular remain most effective approaches mitigating unwanted sequelae diet-induced on brain health. In study, we used high-fat high-sugar (HFHS) mouse model neurodegeneration examine...
Genistein (Gen) and exercise (Exe) have been postulated as potential strategies to ameliorate obesity, inflammation, gut microbiota (GM) with promising results. However, the impact of combination both Exe Gen is yet be investigated. We aimed analyze impacts Exe, Gen, their combined effects on GM inflammation in mice after a 12-week high-fat, high-sugar diet (HFD). Eighty-three C57BL/6 were randomized control, HFD, HFD + or Gen. The V4 region 16S rRNA gene was analyzed Illumina MiSeq. Serum...
Metabolic dysfunction-associated fatty liver disease (MAFLD) is fueled by escalations in both sedentary behavior and caloric intake noted obese type 2 diabetic (T2DM) patients. This study aimed to examine the effects of exercise phytoestrogen genistein mice fed a high fat (60% fat) sugar (55% fructose with 45% sucrose), HFHS diet.Male C57BL/6J were assigned five groups: HFHS, (600 mg/kg diet, HFHS+Gen), moderate (HFHS+Ex), combined (HFHS-Gen+Ex). Control lean standard chow water. Exercise...
Pulmonary hypertension (PAH) is characterized by abnormal vascular remodeling and increased pulmonary artery pressure which lead to right ventricular (RV) hypertrophy heart failure. Resveratrol (3,5,4′-trihydroxy-trans-stilbene), a dietary polyphenol found in the skins seeds of grapes, has been have antioxidant, anti-proliferative anti-fibrotic effects. This study examined effects resveratrol on cardiac trunk remodeling, common plasma markers function rats with PAH was induced male...