A5017642428- Heavy Metal Exposure and Toxicity
- Wnt/β-catenin signaling in development and cancer
- Antimicrobial Resistance in Staphylococcus
- Acute Kidney Injury Research
- Trace Elements in Health
- Antibiotics Pharmacokinetics and Efficacy
- Mercury impact and mitigation studies
- Renal and related cancers
- Cardiac electrophysiology and arrhythmias
- Sepsis Diagnosis and Treatment
- Chronic Kidney Disease and Diabetes
- Heavy metals in environment
- Anesthesia and Sedative Agents
- Diet, Metabolism, and Disease
- Regulation of Appetite and Obesity
- Traditional and Medicinal Uses of Annonaceae
- Alkaloids: synthesis and pharmacology
- Cancer-related gene regulation
- Receptor Mechanisms and Signaling
- Kruppel-like factors research
- Barrier Structure and Function Studies
- Clostridium difficile and Clostridium perfringens research
- Ion channel regulation and function
- Connexins and lens biology
- Chemotherapy-induced organ toxicity mitigation
Midwestern University
2009-2022
Cadmium (Cd) is a nephrotoxic environmental pollutant that causes generalized dysfunction of the proximal tubule characterized by polyuria and proteinuria. Even though effects Cd on kidney have been well-characterized, molecular mechanisms underlying these not fully elucidated. MicroRNAs (miRNAs) are small non-coding RNAs regulate cellular physiologic function modulating gene expression at post-transcriptional level. The goal present study was to determine if affects renal cortex miRNA in...
ABSTRACT Vancomycin has been associated with acute kidney injury (AKI). However, the pharmacokinetic/toxicodynamic relationship for AKI is not well defined. Allometrically scaled vancomycin exposures were used to assess between exposure and AKI. Male Sprague-Dawley rats received clinical-grade in normal saline (NS) as intraperitoneal (i.p.) injections 24- 72-h durations doses ranging 0 200 mg/kg of body weight divided once or twice daily. Urine was collected over protocol's final 24 h. Renal...
Urinary biomarkers are superior to serum creatinine for defining onset and extent of kidney injury. This study classifies the temporal predictive ability vancomycin-induced injury (VIKI) as defined by histopathologic damage. Male Sprague-Dawley rats (n = 125) were randomized receive 150 400 mg/kg body weight/day vancomycin via once or twice daily intraperitoneal injection over 1, 3, 6 days. Urine was collected during 24 h prior euthanasia treated Receiver operating characteristic (ROC)...
Vancomycin has been associated with acute kidney injury in preclinical and clinical settings; however, the precise exposure profiles vancomycin-induced have not defined. We sought to determine pharmacokinetic/pharmacodynamics indices development of using sensitive urinary biomarkers. Male Sprague-Dawley rats received clinical-grade vancomycin or normal saline as an intraperitoneal injection. Total daily doses between 0 400 mg/kg body weight were administered a single dose 2 divided over 24-h...
Abstract Objectives To identify the pharmacokinetic (PK) and toxicodynamic (TD) relationship for vancomycin-induced kidney injury. Methods Male Sprague–Dawley rats received intravenous (iv) vancomycin. Doses ranging from 150 mg/kg/day to 400 were administered as a single or twice-daily injection over 24 h (total protocol duration). Controls iv saline. Plasma was sampled with up eight samples in per rat. Twenty-four hour urine collected assayed injury molecule 1 (KIM-1), osteopontin...
Kratom (Mitragyna speciosa, Korth) is a tree-like plant that indigenous to Southeast Asia. leaf products have been used in traditional folk medicine for their unique combination of stimulant and opioid-like effects. being increasingly the West its reputed benefits treatment pain, depression opioid use disorder. Recently, United States Food Drug Administration Centers Disease Control raised concerns regarding contamination some kratom with toxic metals (Pb Ni) microbes such as Salmonella. To...
Abstract Background Ethidium homodimer is a cell-membrane impermeant nuclear fluorochrome that has been widely used to identify necrotic cells in culture. Here, we describe novel technique for evaluating necrosis of epithelial the proximal tubule involves perfusing ethidium through intact rat kidney. As positive control inducing necrosis, rats were treated with 3.5, 1.75, 0.87 and 0.43 mg/kg mercuric chloride (Hg 2+ , intraperitoneal), treatments which have previously shown rapidly cause...
Cadmium (Cd) is a nephrotoxic environmental pollutant that causes insidious injury to the proximal tubule results in severe polyuria and proteinuria. Cystatin C low molecular weight protein being evaluated as serum urinary biomarker for various types of ischemic renal injury. The objective present study was determine if cystatin might be useful early Cd nephrotoxicity. Male Sprague–Dawley rats were given daily injections up 12 weeks. At 3, 6, 9 weeks, urine samples analyzed C, protein,...
Kratom ( Mitragyna speciosa , Korth.) is an evergreen tree that indigenous to Southeast Asia. When ingested, kratom leaves or decoctions from the have been reported produce complex stimulant and opioid-like effects. For generations, native populations in Asia used products stave off fatigue, improve mood, alleviate pain manage symptoms of opioid withdrawal. Despite long history use Asia, it only within past 10–20 years has emerged as important herbal agent United States, where being for...
ABSTRACT The cadherin/catenin complex serves as an important structural component of adherens junctions in epithelial cells. Under certain conditions, β-catenin can be released from this and interact with transcription factors the nucleus to stimulate expression genes that regulate apoptosis cell cycle control. While studying effects bacterial pathogen Chlamydia trachomatis on human cervical cells culture, we observed C. caused separate each other without detaching their growing surface....
Diabetic obesity in the leptin-deficient ob/ob mouse is associated with weight gain, and hyperglycemia, along hyperinsulinemia. We have previously examined effects of genistein (a naturally occurring isoflavone found soy) on metabolic disturbances demonstrated beneficial (600 mg genistein/kg diet, for 4-weeks) T3 production corticosterone status. The goal this study was to examine whether dietary could prevent, or at least lessen, typical phenotype murine model diabetic-obesity, assess...
Vancomycin induces exposure-related acute kidney injury. However, the pharmacokinetic-toxicodynamic (PK-TD) relationship remains unclear. Sprague-Dawley rats received intravenous (i.v.) vancomycin doses of 300 mg/kg/day and 400 mg/kg/day, divided into once-, twice-, three-times-, or four-times-daily (i.e., QD, BID, TID, QID) over 24 h. Up to 8 samples plus a terminal sample were drawn during 24-h dosing period. Twenty-four-hour urine was collected assayed for injury molecule-1 (KIM-1)....
Vancomycin area under the concentration curve (AUC) is known to predict vancomycin-induced acute kidney injury (AKI). Data were analyzed from a rat model (n = 48) and two prospective clinical studies (PROVIDE [n 263] CAMERA2 291]). A logit-link was used calculate multiplicative factors between probability of AKI in rat. The 2.7 4.2 times more sensitive AUCs 199.5 794.3 mg·h/liter, respectively.
Cd (Cd) is a nephrotoxic environmental pollutant that causes generalized proximal tubule dysfunction. Even though the specific mechanisms by which damages kidney have yet to be fully elucidated, there evidence suggest some of these effects may result from ability alter levels and function metals such as Cu, Se, Zn Fe within kidney. In order further explore this issue, we examined subchronic exposure on tissue panel (Ca, Fe, K, Mg, Na, Se Zn) in rat renal cortex. Adult male Sprague-Dawley...