A5026127519- Pancreatic and Hepatic Oncology Research
- Adenosine and Purinergic Signaling
- Cancer Immunotherapy and Biomarkers
- Pancreatitis Pathology and Treatment
- Gallbladder and Bile Duct Disorders
- Adolescent and Pediatric Healthcare
- Lung Cancer Research Studies
- Gastric Cancer Management and Outcomes
- Peptidase Inhibition and Analysis
- Neuroendocrine Tumor Research Advances
- CAR-T cell therapy research
- Renal cell carcinoma treatment
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Screening and Detection
- Angiogenesis and VEGF in Cancer
- Cancer Genomics and Diagnostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Axon Guidance and Neuronal Signaling
- Colorectal Cancer Surgical Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Neuroblastoma Research and Treatments
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Esophageal and GI Pathology
- Palliative Care and End-of-Life Issues
Baylor College of Medicine
2016-2025
The University of Texas MD Anderson Cancer Center
2006-2023
Dan L Duncan Comprehensive Cancer Center
2015-2023
National Institutes of Health
2023
Baylor University Medical Center
2023
Baylor University
2021
Anesthesiology and Surgical Oncology Research Group
2020
Improvement Service
2019
Hospital El Cruce
2018
Pancreas Centre (Canada)
2017
5-Fluorouracil (5FU) and oxaliplatin are standard therapy for metastatic colorectal cancer (CRC), but the development of chemoresistance is inevitable. Because stem cells (CSC) hypothesized to be chemoresistant, we investigated CSC properties in newly developed chemoresistant CRC cell lines sought identify targets therapy. The human line HT29 was exposed increasing doses 5FU (HT29/5FU-R) or (HT29/OxR) achieve resistance at clinically relevant doses. Western blotting flow cytometry were done...
Abstract Purpose: Epithelial-to-mesenchymal transition (EMT) is a process whereby cells acquire molecular alterations that facilitate cell motility and invasion. In preliminary studies, we observed oxaliplatin-resistant (OxR) colorectal cancer (CRC) underwent morphologic changes suggestive of migratory phenotype, leading us to hypothesize OxR CRC undergo EMT. Experimental Design: The human lines KM12L4 HT29 were exposed increasing doses oxaliplatin establish stable resistant oxaliplatin....
To test by randomized prospective multicenter trial the hypothesis that pancreaticoduodenectomy (PD) without use of intraperitoneal drainage does not increase frequency or severity complications.Some surgeons have abandoned drains placed during pancreas resection.We 137 patients to PD with (n = 68, drain group) and 69, no-drain compared safety this approach spectrum complications between 2 groups.There were no differences cohorts in demographics, comorbidities, pathology, pancreatic duct...
Neuropilin-2 (NRP2) is a high-affinity kinase-deficient receptor for vascular endothelial growth factor (VEGF) and semaphorin 3F. We investigated its function in human colorectal cancers.Immunohistochemistry immunoblotting were used to assess NRP2 expression levels tumors cancer cell lines, respectively. HCT-116 cells stably transfected with short hairpin RNA (shRNAs) against or control shRNAs assayed proliferation by the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium...
Objective: The objective of this study was to test the hypothesis that distal pancreatectomy (DP) without intraperitoneal drainage does not affect frequency grade 2 or higher complications. Background: use routine drains during DP is controversial. Prior study, no prospective trial focusing on has been reported. Methods: Patients undergoing for all causes at 14 high-volume pancreas centers were preoperatively randomized placement a drain drain. Complications and their severity tracked 60...
The ampulla of Vater is a complex cellular environment from which adenocarcinomas arise to form group histopathologically heterogenous tumors. To evaluate the molecular features these tumors, 98 ampullary were evaluated and compared 44 distal bile duct 18 duodenal adenocarcinomas. Genomic analyses revealed mutations in WNT signaling pathway among half patients all three irrespective their origin histological morphology. These tumors characterized by high frequency inactivating ELF3, rate...
Intraoperative delineation of tumor margins is critical for effective pancreatic cancer surgery. Yet, intraoperative frozen section analysis a time-consuming and often challenging procedure that can yield confounding results due to histologic heterogeneity tissue-processing artifacts. We have previously described the development MasSpec Pen technology as handheld mass spectrometry-based device nondestructive tissue analysis. Here, we evaluated usefulness diagnosis ductal adenocarcinoma based...
Abstract The microenvironment that surrounds pancreatic ductal adenocarcinoma (PDAC) is profoundly desmoplastic and immunosuppressive. Understanding triggers of immunosuppression during the process tumorigenesis would aid in establishing targets for effective prevention therapy. Here, we interrogated differential molecular mechanisms dependent on cell origin subtype promote PDAC initiation established tumors. Transcriptomic analysis cell-of-origin–dependent epithelial gene signatures...
Abstract Purpose. Neuropilin-2 (NRP-2) is a coreceptor for vascular endothelial growth factor (VEGF) on cells. NRP-2 overexpressed in pancreatic ductal adenocarcinoma (PDAC) cells relative to nonmalignant epithelium. This study determined the role of PDAC Experimental Design. expression was reduced with stable short-hairpin RNA (shRNA) transfection. Western blotting done evaluate signaling intermediates. Migration and invasion studies were carried out Boyden chambers. Anchorage-independent...
AIMTo characterize incidence and risk factors for delayed gastric emptying (DGE) following pancreaticoduodenectomy examine its implications on healthcare utilization. METHODSA prospectively-maintained database was reviewed.DGE classified using International Study Group of Pancreatic Surgery criteria.Patients who developed DGE those did not were compared. RESULTSTwo hundred seventy-six patients underwent (PD) (> 80% pyloruspreserving, antecolic-reconstruction). in 49 (17.8%): 5.1% grade B,...
Pancreatic cystic lesions (PCLs) may be precancerous. Those likely to harbor high-grade dysplasia (HGD) or pancreatic cancer (PC) are targets for surgical resection. Current algorithms predict advanced neoplasia (HGD/PC) in PCLs lack diagnostic accuracy. In tissue and cyst fluid (CF) from PCLs, we sought identify validate novel methylated DNA markers (MDMs) that discriminate HGD/PC low-grade (LGD) no (ND).From an unbiased whole-methylome discovery approach using predefined selection criteria...
This study aims to evaluate the prognostic value of MUC expression in US GC patients. A total 70 tumor specimens were collected from patients who underwent surgery or endoscopic resection between 2013 and 2019 at a tertiary referral center US. status including MUC1, MUC2, MUC5AC, MUC6 was evaluated by immunohistochemical staining. The positive rates 71.4%, 78.6%, 74.3%, 33.3%, respectively. Patients with MUC1 had significantly higher rate aggressive pathologic features diffuse-type cancer...
Unresectable metastatic colorectal cancer (CRC) can be rendered resectable with systemic chemotherapy in approximately 20% of cases. Most patients CRC receive the addition targeted therapy anti-vascular endothelial growth factor (VEGF) or anti-epidermal receptor (EGFR) antibodies. We sought to determine whether anti-VEGF (VEGFR) anti-EGFR would impair liver regeneration after partial hepatectomy (PH) mice.Mice underwent either 66% PH sham laparotomy. In first experiment, mice group were...
Abstract Purpose: Gastrointestinal neuroendocrine tumors (NET) are rare heterogeneous that hypersecrete neuropeptides. The scarcity of good gastrointestinal NET models has limited the ability to study potential therapeutic agents. We describe and characterize establishment a human midgut carcinoid tumor cell line 2 (CNDT2). Experimental Design: Tumor cells (CNDT2) were isolated from liver metastasis patient with primary ileal carcinoid. After 9 weeks in culture, plated soft agar, single...