Angelika N. Christ
A5077058409- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Evolution and Genetic Dynamics
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Genetic factors in colorectal cancer
- Lung Cancer Treatments and Mutations
- Congenital heart defects research
- Neuroendocrine Tumor Research Advances
- Nutrition, Genetics, and Disease
- Genomics and Rare Diseases
- Genetics, Bioinformatics, and Biomedical Research
- Ovarian cancer diagnosis and treatment
- Genomic variations and chromosomal abnormalities
- Lung Cancer Research Studies
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
The University of Queensland
2014-2023
Korea Advanced Institute of Science and Technology
2020
Universitätsklinikum Erlangen
2020
Research Institute for Bioscience and Biotechnology
2017
Royal Women's Hospital
2015
The University of Melbourne
2015
Oesophageal adenocarcinoma (EAC) incidence is rapidly increasing in Western countries. A better understanding of EAC underpins efforts to improve early detection and treatment outcomes. While large exome sequencing date have found recurrent loss-of-function mutations, oncogenic driving events been underrepresented. Here we use a combination whole-genome (WGS) single-nucleotide polymorphism-array profiling show that genomic catastrophes are frequent EAC, with almost third (32%, n=40/123)...
Abstract Heterogeneous subtypes of cancer-associated fibroblasts (CAFs) coexist within pancreatic cancer tissues and can both promote restrain disease progression. Here, we interrogate how cells harboring distinct alterations in p53 manipulate CAFs. We reveal the existence a p53-driven hierarchy, where with gain-of-function (GOF) mutant educate dominant population CAFs that establish pro-metastatic environment for GOF null alike. also demonstrate educated by may be reprogrammed either or...
The importance of epigenetic modifications such as DNA methylation in tumorigenesis is increasingly being appreciated. To define the genome-wide pattern pancreatic ductal adenocarcinomas (PDAC), we captured profiles 167 untreated resected PDACs and compared them to a panel 29 adjacent nontransformed pancreata using high-density arrays. A total 11,634 CpG sites associated with 3,522 genes were significantly differentially methylated (DM) PDAC capable segregating from non-malignant pancreas,...
Abstract Treatment options for patients with brain metastases ( BMs ) have limited efficacy and the mortality rate is virtually 100%. Targeted therapy critically under‐utilized, our understanding of mechanisms underpinning metastatic outgrowth in limited. To address these deficiencies, we investigated genomic transcriptomic landscapes 36 from breast, lung, melanoma oesophageal cancers, using DNA copy‐number analysis exome‐ RNA ‐sequencing. The key findings were as follows. (a) Identification...
Heterogeneity of cell states represented in pluripotent cultures has not been described at the transcriptional level. Since gene expression is highly heterogeneous between cells, single-cell RNA sequencing can be used to identify how individual cells function. Here, we present results from analysis data 18,787 WTC-CRISPRi human induced stem cells. We developed an unsupervised clustering method and, through this, identified four subpopulations distinguishable on basis their state, including a...
Somatic mutation calling from next-generation sequencing data remains a challenge due to the difficulties of distinguishing true somatic events artifacts arising PCR, errors or mis-mapping. Tumor cellularity purity, sub-clonality and copy number changes also confound identification against background germline variants. We have developed heuristic strategy software (http://www.qcmg.org/bioinformatics/qsnp/) for in samples with low tumor content we show superior sensitivity precision our...
Conventional dendritic cells (cDCs) are traditionally subdivided into cDC1 and cDC2 lineages. Batf3 is a cDC1-required transcription factor, we observed that Batf3−/− mice harbor population of cDC1-like co-expressing cDC2-associated surface molecules. Using single-cell RNA sequencing with integrated cell protein expression (CITE-seq), found mitotic immature showed reduced features increased levels features. In wild type, also proportion mature expressing characteristic to overall cDC state...
Influenza A virus (IAV) causes a wide range of extrarespiratory complications. However, the role host factors in these complications influenza infection remains to be defined.Here, we sought use transcriptional profiling, virology, histology, and echocardiograms investigate high-fat diet IAV-associated cardiac damage.Transcriptional profiling showed that, compared their low-fat counterparts (LF mice), mice fed (HF mice) had impairments inflammatory signaling lung heart after IAV infection....
Genetic variation modulates gene expression transcriptionally or post-transcriptionally, and can profoundly alter an individual's phenotype. Measuring allelic differential at heterozygous loci within individual, a phenomenon called allele-specific (ASE), assist in identifying such factors. Massively parallel DNA RNA sequencing advances bioinformatic methodologies provide outstanding opportunity to measure ASE genome-wide. In this study, matched sequencing, genotyping arrays computationally...
The hematopoietic prostaglandin D2 synthase has a proinflammatory effect in range of diseases, including allergic asthma, where its product (PGD2) role regulating many the hallmark disease characteristics. Here we describe development and characterization novel series inhibitors with potency similar to that known inhibitors. Compounds N-benzhydryl-5-(3-hydroxyphenyl)thiophene-2-carboxamide (compound 8) N-(1-amino-1-oxo-3-phenylpropan-2-yl)-6-(thiophen-2-yl)nicotinamide 34) demonstrated low...
Abstract The majority of genetic loci underlying common disease risk act through changing genome regulation, and are routinely linked to expression quantitative trait loci, where gene is measured using bulk populations mature cells. A crucial step that missing evidence variation in the these genes as cells progress from a pluripotent state. This especially important for cardiovascular disease, cardiac have limited properties renewal postneonatal. To investigate dynamic changes across...
Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment single-cell transcriptomic profiling with integrated cell surface protein expression CITE-seq. This protocol provides a general transferrable road map for different tissues species. For complete details on use execution this protocol, please refer Lukowski et al. (2021).
Abstract Heterogeneity of cell states represented in pluripotent cultures have not been described at the transcriptional level. Since gene expression is highly heterogeneous between cells, single-cell RNA sequencing can be used to identify how individual cells function. Here, we present results from analysis data 18,787 WTC CRISPRi human induced stem cells. We developed an unsupervised clustering method, and through this identified four subpopulations distinguishable on basis their state...
Somatic rearrangements, which are commonly found in human cancer genomes, contribute to the progression and maintenance of cancers. Conventionally, verification somatic rearrangements comprises many manual steps Sanger sequencing. This is labor intensive when verifying a large number cohort. To increase throughput, we devised high-throughput workflow that utilizes benchtop next-generation sequencing in-house bioinformatics tools link laboratory processes. In proposed workflow, primers...