Barsha Poudel

ORCID: 0000-0003-0435-276X
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About
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Research Areas
  • Plant Pathogens and Fungal Diseases
  • Wheat and Barley Genetics and Pathology
  • Plant Disease Management Techniques
  • Plant Disease Resistance and Genetics
  • Genomics and Phylogenetic Studies
  • Yeasts and Rust Fungi Studies
  • Plant biochemistry and biosynthesis
  • Cancer Genomics and Diagnostics
  • Plant tissue culture and regeneration
  • CRISPR and Genetic Engineering
  • Animal Genetics and Reproduction
  • Microbial Metabolic Engineering and Bioproduction
  • Plant Pathogens and Resistance
  • RNA modifications and cancer
  • Ovarian cancer diagnosis and treatment
  • Viral Infectious Diseases and Gene Expression in Insects
  • Plant Pathogenic Bacteria Studies
  • Chromosomal and Genetic Variations
  • Plant Virus Research Studies
  • Muscle and Compartmental Disorders
  • Biomedical Text Mining and Ontologies
  • Advanced Chemical Sensor Technologies
  • Plant and fungal interactions
  • Turfgrass Adaptation and Management
  • Genetic Mapping and Diversity in Plants and Animals

University of Southern Queensland
2017-2024

Curtin University
2017

Agriculture Victoria
2017

The University of Queensland
2015

Royal Women's Hospital
2015

The University of Melbourne
2015

Wageningen University & Research
2012

10.1038/nature14169 article EN Nature 2015-02-24

In bacteria, plasmids can confer the ability to cause disease. Although they potentially vary in copy number, little has been reported on dynamics of plant pathogenic bacteria. Pathogenicity bacterium Curtobacterium flaccumfaciens pv. ( Cff ), which causes foliar disease leguminous crops, including mung bean Vigna radiata previously linked a plasmid. This study explored variation plasmid number among genetically diverse collection 25 isolates using purposely designed quantitative PCR assays...

10.1101/2025.01.22.634406 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2025-01-25

example, Sipos et al. (2017) showed that genome evolution in the genus was predominantly caused by gene family expansion.Kolesnikova (2019) assembled complete mitochondrial genomes of A. borealis, gallica, sinapina, and solidipes found a high degree variation size, content genomic organization among these phylogenetically closely related species.Recently, first chromosome-level Armillaria assembly became available, revealing genome-wide recombination ostoyae (Heinzelmann 2020).Sequenced...

10.1186/s43008-022-00104-3 article EN cc-by IMA Fungus 2022-11-21

Pyrenophora teres f. and P. maculata cause net form spot form, respectively, of blotch on barley (Hordeum vulgare). The two forms reproduce sexually, producing hybrids with genetic pathogenic variability. Phenotypic identification is challenging because lesions induced by host plants resemble either or maculata. In this study, 12 sequence-specific polymerase chain reaction markers were developed based expressed regions spread across the genome. primers validated using 210 isolates, 2...

10.1094/phyto-11-16-0396-r article EN other-oa Phytopathology 2017-04-14

Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed draft genome of M. isolate BRIP 70780a from sorghum, using long-read native DNA MinION sequencing, which was error-corrected short-read Illumina MiSeq reads. The genome, consisting 22 contigs with an N50 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, valuable resource to aid future studies population...

10.1094/mpmi-12-19-0356-a article EN other-oa Molecular Plant-Microbe Interactions 2020-02-25

Despite the substantial economic impact of Curtobacterium flaccumfaciens pv. on legume production worldwide, genetic basis its pathogenicity and potential host association is poorly understood. The high-quality reference genome assemblies C. strains associated with different hosts sheds light pathogenic variability association. Moreover, study recent outbreaks bacterial wilt microevolution pathogen in Australia requires access to genomes that are sufficiently closely related population being...

10.1094/mpmi-05-21-0116-a article EN cc-by-nc-nd Molecular Plant-Microbe Interactions 2021-06-29

Purpose: Phosphatidylserine exposed on live and motile sperm is necessary for sperm:egg fusion, emerging data suggest it a biomarker fertilization competent sperm. Men with varicocele often display infertility, one of the known causes poor quality. Here, we sought to determine whether percentage phosphatidylserine-positive (PS + sperm) could serve as reliable indicator male fertility. Materials Methods: Semen samples from cryo-banked pregnancy-proven donors, self-identified...

10.1097/ju9.0000000000000221 article EN cc-by-nc-nd JU Open Plus 2024-11-01

Net form of net blotch caused by Pyrenophora teres f. (Ptt) is a major foliar disease barley ( Hordeum vulgare ) worldwide. Knowledge the evolution Ptt pathogen populations important for development durable host‐plant resistance. This study was conducted to investigate changes in genetic structure population within field during three cropping years. The susceptible cultivar Henley inoculated with isolate NB050. Leaf samples were collected years 2013–15 and 174 single spore isolates stored....

10.1111/ppa.13035 article EN Plant Pathology 2019-04-23

Charcoal rot is an important soilborne disease caused by a range of Macrophomina species, which affects broad commercially crops worldwide. Even though species are fungal pathogens substantial economic importance, their mechanism pathogenicity and host spectrum poorly understood. There urgent need to better understand the biology, epidemiology, evolution which, in turn, will aid improving charcoal management strategies. Here, we present first high-quality genome assembly annotation tecta...

10.1093/gbe/evac081 article EN cc-by-nc Genome Biology and Evolution 2022-05-31

Common rust of maize (CR), caused by Puccinia sorghi Schwein., is found in all growing regions across the globe. In recent years, an increase incidence and severity CR has been observed Australia. The challenges posed a diverse population P. to breeding programs strategies required deploy durable resistance were explored. Preliminary studies diversity conducted phenotyping five most isolates against lines carrying no genes one four genes. results showed that further genotyping required....

10.1094/phytofr-12-22-0151-r article EN cc-by-nc-nd PhytoFrontiers™ 2023-03-09

Net or web blotch (NB), caused by Didymella arachidicola, is an emerging threat causing a significant reduction in yield of peanuts cooler production regions globally, including Australia, China, and Southern Africa. Host resistance against NB poorly understood, with field screenings reliant on favorable climatic conditions. A rapid effective phenotyping method essential to differentiate between resistant susceptible breeding lines improve selection accuracy. In this study, greenhouse...

10.1094/phytofr-07-22-0078-r article EN cc-by-nc-nd PhytoFrontiers™ 2023-03-14
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