A5008597480- Single-cell and spatial transcriptomics
- Machine Learning in Bioinformatics
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Congenital heart defects research
- Tryptophan and brain disorders
- Cancer Immunotherapy and Biomarkers
- Genomics and Phylogenetic Studies
- Long-Term Effects of COVID-19
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- Protein Structure and Dynamics
- Immune Cell Function and Interaction
- Bacterial Infections and Vaccines
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Developmental Biology and Gene Regulation
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Advanced Breast Cancer Therapies
- Computational Drug Discovery Methods
First Affiliated Hospital of Guangzhou Medical University
2023-2025
State Key Laboratory of Respiratory Disease
2023-2025
Guangzhou Experimental Station
2022-2024
Dana-Farber Cancer Institute
2018-2023
Guangzhou Medical University
2022-2023
Harvard University
2020-2022
Center for Excellence in Molecular Cell Science
2016-2021
Chinese Academy of Sciences
2016-2021
Shanghai Institutes for Biological Sciences
2016-2021
Shanghai Institute of Nutrition and Health
2019-2021
Recent progress in single-cell technologies has enabled the identification of all major cell types mouse. However, for most types, regulatory mechanism underlying their identity remains poorly understood. By computational analysis recently published mouse atlas data, we have identified 202 regulons whose activities are highly variable across different and more importantly, predicted a small set essential regulators each type Systematic validation by automated literature data mining provides...
Abstract Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity different organisms, cross-species comparisons landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating comprehensive dataset > 2.6 million single cells, constructed a identified signatures common pathways that...
Abstract Motivation: Systematic dissection of the ubiquitylation proteome is emerging as an appealing but challenging research topic because significant roles play not only in protein degradation also many other cellular functions. High-throughput experimental studies using mass spectrometry have identified sites, primarily from eukaryotes. However, vast majority sites remain undiscovered, even well-studied systems. Because spectrometry–based approaches for identifying events are costly,...
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, has been associated with neurological and neuropsychiatric illness in many individuals. We sought to further our understanding of the relationship between brain tropism, neuro-inflammation, host immune response COVID-19 cases. Three regions (dorsolateral prefrontal cortex, medulla oblongata, choroid plexus) from 5 patients 4 controls were examined. The presence virus was...
Abstract CDK4/6 inhibitors are approved to treat breast cancer and in trials for other malignancies. We examined inhibition mouse human CD8+ T cells during early stages of activation. Mice receiving tumor-specific treated with displayed increased T-cell persistence immunologic memory. upregulated MXD4, a negative regulator MYC, both cells. Silencing Mxd4 or Myc demonstrated the importance this axis memory formation. used single-cell transcriptional profiling receptor clonotype tracking...
In vivo, some proteins exist as monomers and others oligomers. Oligomers can be further classified into homo-oligomers (formed by identical subunits) hetero-oligomers different subunits), they form the structural components of various biological functions, including cooperative effects, allosteric mechanism ion-channel gating. Therefore, with avalanche protein sequences generated in post-genomic era, it is very important for both basic research pharmaceutical industry to acquire possible...
Protein methylation is predominantly found on lysine and arginine residues, carries many important biological functions, including gene regulation signal transduction. Given their involvement in expression, protein regulatory enzymes are implicated a variety of human disease states such as cancer, coronary heart neurodegenerative disorders. Thus, identification sites can be very helpful for the drug designs various related diseases. In this study, we developed method called PMeS to improve...
Post-translational lysine methylation and acetylation are two major modifications of residues. They play critical roles in various biological processes, especially gene regulation. Identification protein sites would be a foundation for understanding their modification dynamics molecular mechanism. This work presents method called PLMLA that incorporates sequence information, secondary structure amino acid properties to predict residues whole sequences. We apply an encoding scheme based on...
In vertebrates, hematopoiesis occurring in different niches is orchestrated by intrinsic and extrinsic regulators. Previous studies have revealed numerous linear planar regulatory mechanisms. However, a multi-dimensional transcriptomic atlas of any given hematopoietic organ has not yet been established. Here, we use multiple RNA sequencing (RNA-seq) approaches, including cell type-specific, temporal bulk RNA-seq, vivo GEO-seq, single-cell RNA-seq (scRNA-seq), to characterize the detailed...
Immunoglobulin A (IgA) is the major secretory immunoglobulin isotype found at mucosal surfaces, where it regulates microbial commensalism and excludes luminal factors from contacting intestinal epithelial cells (IECs). IgA induced by both T cell-dependent -independent (TI) pathways. However, little known about TI regulation. We report that IEC endoplasmic reticulum (ER) stress induces a polyreactive response, which protective against enteric inflammation. ER causes microbiota-independent...
The forward genetic screen is a powerful, unbiased method to gain insights into biological processes, yet this approach has infrequently been used in vivo mammals because of high resource demands. Here, we use somatic Cas9 mutagenesis perform an mice identify regulators cardiomyocyte (CM) maturation, the coordinated changes phenotype and gene expression that occur neonatal CMs. We discover validate number transcriptional process. Among these are RNF20 RNF40, which form complex...
Protein lysine acetylation is a type of reversible post-translational modification that plays vital role in many cellular processes, such as transcriptional regulation, apoptosis and cytokine signaling. To fully decipher the molecular mechanisms acetylation-related biological an initial but crucial step recognition acetylated substrates corresponding sites. In this study, we developed position-specific method named PSKAcePred for prediction based on support vector machines. The residues...
Preimplantation embryogenesis encompasses several critical events including genome reprogramming, zygotic activation (ZGA), and cell-fate commitment. The molecular basis of these processes remains obscure in primates which there is a high rate embryo wastage. Thus, understanding the factors involved reprogramming ZGA might help reproductive success during this susceptible period early development generate induced pluripotent stem cells with greater efficiency. Moreover, explaining...
Abstract Tumor-infiltrating myeloid-derived suppressor cells (MDSC) are associated with poor survival outcomes in many human cancers. MDSCs inhibit T cell–mediated tumor immunity part because they strongly T-cell function. However, whether early or later steps of activation is not well established. Here we show that inhibited proliferation and induced apoptosis CD8+ even the presence dendritic (DC) presenting a high-affinity cognate peptide. This inhibitory effect was also observed delayed...
Abstract Mammalian embryos exhibit sophisticated cellular patterning that is intricately orchestrated at both molecular and level. It has recently become apparent cells within the animal body display significant heterogeneity, in terms of their properties spatial distributions. However, current transcriptomic profiling either lacks three-dimensional representation or limited its ability to capture complexity embryonic tissues organs. Here, we present a atlas all major organs day 13.5 mouse...