A5065811811- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Cell Image Analysis Techniques
- Tendon Structure and Treatment
- Cancer-related molecular mechanisms research
- Shoulder Injury and Treatment
- Knee injuries and reconstruction techniques
- Gene Regulatory Network Analysis
- Immune cells in cancer
- Plant Pathogenic Bacteria Studies
- Selenium in Biological Systems
- Ferroptosis and cancer prognosis
- Trace Elements in Health
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Shoulder and Clavicle Injuries
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Genomics and Phylogenetic Studies
- Cardiac Valve Diseases and Treatments
- Legume Nitrogen Fixing Symbiosis
- Neutropenia and Cancer Infections
- Genetics, Aging, and Longevity in Model Organisms
- Vehicle Noise and Vibration Control
Fudan University
2023-2025
Huashan Hospital
2023-2025
Zhejiang University
2019-2024
Shenzhen University
2024
First Affiliated Hospital Zhejiang University
2020-2023
China Agricultural University
2021-2023
Xiamen University
2023
Second Affiliated Hospital of Zhejiang University
2023
Ministry of Agriculture and Rural Affairs
2023
Sailun Jinyu Group (China)
2023
Objective Hepatocellular carcinoma (HCC) is heterogeneous, especially in multifocal tumours, which decreases the efficacy of clinical treatments. Understanding tumour heterogeneity critical when developing novel treatment strategies. However, a comprehensive investigation HCC lacking, and available evidence regarding has not led to improvements practice. Design We harvested 42 samples from eight patients evaluated using whole-exome sequencing, RNA mass spectrometry-based proteomics...
Abstract Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity different organisms, cross-species comparisons landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating comprehensive dataset > 2.6 million single cells, constructed a identified signatures common pathways that...
Abstract Background Acute myeloid leukemia (AML) is a fatal hematopoietic malignancy and has prognosis that varies with its genetic complexity. However, there been no appropriate integrative analysis on the hierarchy of different AML subtypes. Methods Using Microwell-seq, high-throughput single-cell mRNA sequencing platform, we analyzed cellular bone marrow samples from 40 patients 3 healthy donors. We also used single-molecule real-time (SMRT) to investigate clonal heterogeneity cells....
Overwhelming evidences suggest oxidative stress is a major cause of sperm dysfunction and male infertility. Zinc an important non-enzymatic antioxidant with wide range biological functions plays significant role in preserving fertility. Notably, zinc trafficking through the cellular intracellular membrane mediated by specific families transporters, i.e., SLC39s/ZIPs SLC30s/ZnTs. However, their expression function were rarely evaluated germ cells. The aim this study to determine characterize...
The tumor microenvironment heterogeneity of papillary thyroid cancer (PTC) is poorly characterized. relationship between PTC and Hashimoto thyroiditis (HT) also in doubt. Here, we used single-cell RNA sequencing to map the transcriptome landscape from eight patients, which three were concurrent with HT. Predicted copy number variation epithelial cells mesenchymal revealed distinct molecular signatures carcinoma cells. Carcinoma demonstrated intertumoral based on BRAF V600E mutation or lymph...
Serum microRNAs (miRNAs) may serve as biomarkers in various cancers. Our study aims to explore the roles of miR-497 and miR-1246 hepatocellular carcinoma (HCC). The expression levels were measured by RT-PCR. A correlation analysis was conducted between clinicopathological characteristics patients. receiver operating characteristic (ROC) curve applied evaluate diagnostic efficacy HCC. In addition, bioinformatics tools also utilized predict potential targets miR-1246. level HCC significantly...
Immune checkpoint inhibitors (ICIs) offer new options for the treatment of patients with solid cancers worldwide. The majority colorectal (CRC) are proficient in mismatch-repair (pMMR) genes, harbors few tumor antigens and is insensitive to ICIs. These tumors often found be immune-deserted. We hypothesized that forcing immune cell infiltration into microenvironment followed by ignition PD1 blockade may initiate a positive cycle can boost antitumor immunity. Bioinformatics using public...
Background: Augmented repair is an alternative strategy for the treatment of acute ligament and tendon injuries that imparts time-zero biomechanical strength to allow early loading, thereby protecting repaired structures during healing process. Purpose: To investigate properties biological process after suture anterior cruciate (ACL) tears with polyethylene terephthalate (PET) augmentation compare findings those obtained without PET augmentation. Study Design: Controlled laboratory study....
Abstract Collagen-based biomaterials are gaining prominence in tissue engineering, attributed to their remarkable biocompatibility, inherent biodegradability, and unparalleled capacity facilitate repair regeneration. However, the ability dynamically visualize quantitatively assess collagen degradation vivo remains a critical challenge, hindering development of optimized for clinical applications. To address this, novel approach was developed monitor injury microenvironment by conjugating...
Abstract The Mexican axolotl ( Ambystoma mexicanum ) is a well-established tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, process that triggers many dramatic changes in diverse organs, accompanied by gradually decline of their capacity lifespan. However, the molecular regulation cellular metamorphosed are still poorly investigated. Here, we develop single-cell sequencing method based on combinatorial hybridization to...
Abstract Tumor heterogeneity and its drivers impair tumor progression cancer therapy. Single‐cell RNA sequencing is used to investigate the of ecosystems. However, most methods scRNA‐seq amplify termini polyadenylated transcripts, making it challenging perform total analysis somatic mutation analysis.Therefore, a high‐throughput high‐sensitivity method called snHH‐seq developed, which combines random primers preindex strategy in droplet microfluidic platform. This innovative allows for...
Penicillin-binding proteins (PBPs) are considered essential for bacterial peptidoglycan biosynthesis and cell wall assembly. Clavibacter michiganensis is a representative Gram-positive species that causes canker in tomato. pbpC plays significant role maintaining morphological characteristics stress responses C. michiganensis. The current study demonstrated the deletion of commonly enhances pathogenicity revealed mechanisms through which this occurs. expression interrelated virulence genes,...
Background: Extracorporeal shock wave therapy (ESWT) promotes tissue healing by modulating inflammation, which has implications for meniscal tear in the avascular zone. Purpose: To evaluate effects of a single dose radial ESWT on process and inflammation meniscus knee joints after tears Study Design: Controlled laboratory study. Methods: Avascular were induced medial (MM) 72 Sprague-Dawley rats. One week postoperatively, rats received session with Power+ handpiece (ESWT group; n = 36) or...
Abstract The liver is the most tolerogenic of transplanted organs. However, mechanisms underlying transplant tolerance are not well understood. comparison between transplantation and heart/kidney rejection will deepen our understanding in solid Here, we built a mouse model liver, heart kidney allograft performed single‐cell RNA sequencing 66,393 cells to describe cell composition immune interactions at early stage or rejection. We also bulk RNA‐seq allografts from Day 7 60...
With the development of single-cell RNA sequencing (scRNA-seq) technology, analysts need to integrate hundreds thousands cells with multiple experimental batches. It is becoming increasingly difficult for users select best integration methods remove batch effects. Here, we compared advantages and limitations four commonly used Scanpy-based batch-correction using two representative large-scale scRNA-seq datasets. We quantitatively evaluated performance efficiency. Furthermore, discussed...
Abstract Background Xanthomonas campestris pv. c ampestris (Xcc) is an important seed-borne plant pathogenic bacteria that can cause a serious threat to cruciferous crops. Bacteria enter into the viable but non-culturable (VBNC) state under stress conditions, and potential risks agricultural production because VBNC bacterial cells will evade culture-based detection. However, little known about mechanism of VBNC. Our previous study showed Xcc could be induced by copper ion (Cu 2+ ). Results...
This paper studies pattern noise in original equipment fitment through four approaches, including pitch arrangement optimization, pipe resonance experiment, orthogonal experimental design of tread transverse groove, and block shift simulation. An in-cab test is conducted to verify the effectiveness. Results show an 8.7% reduction total harmonic energy 12% decrease highest peak optimization. introduces 3-Sum concept measure difficulty exciting a certain harmonic. Meanwhile, optimal width...