A5044115025- Wnt/β-catenin signaling in development and cancer
- Single-cell and spatial transcriptomics
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- interferon and immune responses
- Microbial Inactivation Methods
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Planarian Biology and Electrostimulation
- MicroRNA in disease regulation
- Congenital heart defects research
- Extracellular vesicles in disease
- Hedgehog Signaling Pathway Studies
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
Guangdong Academy of Medical Sciences
2021-2025
Guangdong Provincial People's Hospital
2021-2025
Southern Medical University
2023-2025
South China Normal University
2021-2023
Zhejiang University
2021
Xinxiang Medical University
2014-2019
Jena University Hospital
2016
Friedrich Schiller University Jena
2015
Stanford University
1996
The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct distribution, features, functions. identified an injury-induced ependymoglial cluster at the wound site as a progenitor population for potential replenishment lost...
The axolotl is broadly used in regenerative, developmental, and evolutionary biology research. Targeted gene knock-in crucial for precision transgenesis, enabling disease modeling, visualization, tracking, functional manipulation of specific cells or genes interest (GOIs). Existing CRISPR/Cas9-mediated homology-independent method often causes "scars/indels" at integration junctions. Here, we develop a semi-homology-directed recombination (HDR) using donor construct containing single homology...
Abstract The Mexican axolotl ( Ambystoma mexicanum ) is a well-established tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, process that triggers many dramatic changes in diverse organs, accompanied by gradually decline of their capacity lifespan. However, the molecular regulation cellular metamorphosed are still poorly investigated. Here, we develop single-cell sequencing method based on combinatorial hybridization to...
The nuclear protein v-Myb, encoded by the avian myeloblastosis virus (AMV), can induce acute monoblastic leukemia in vivo and transform chicken myelomonocytic cells culture. N terminus of v-Myb functions as DNA-binding domain, multiple central C-terminal regions this have been reported to function transcriptional activation model reporter genes. We showed previously that a domain (amino acids 296 371) is required for transformation primary cells. In study, we now analyzed series mutants...
Pax3 and Pax7 are closely related transcription factors that widely expressed in the developing nervous system somites. In CNS, both genes dorsal part of neural tube during development. involved sonic hedgehog (Shh) signaling pathway inhibited by Shh overexpression. The present study confirms vivo overexpression represses expression Pax7, whereas endogenously enhances ectopically induces chicken spinal cord. Overexpression endogenous cadherin-7, a member cadherin family morphogenetic genes,...
To investigate the variance of exogenous gene expression driven by different promoters in vivo electroporation, 3 plasmid vectors carrying were selected, and their driving strength was compared developing chicken embryos.The included: 1) CAG promoter (containing cytomegalovirus (CMV) immediate early enhancer β-actin promoter), 2) CMV (the human region enhancer), 3) SV40 (Simian virus 40).The intensity GFP detected fluorescence microscopy.The results clearly showed that reporter differed...
Axolotl (Ambystoma mexicanum) is an excellent model for investigating regeneration, the interaction between regenerative and developmental processes, comparative genomics, evolution. The brain, which serves as material basis of consciousness, learning, memory, behavior, most complex advanced organ in axolotl. modulation transcription factors a crucial aspect determining function diverse regions within brain. There is, however, no comprehensive understanding gene regulatory network axolotl...
Pax3 is a transcription factor that belongs to the paired box family. In developing spinal cord it expressed in dorsal commissural neurons, which project ascending axons contralaterally form proper cord-brain circuitry. While has been shown induces cell aggregation vitro, little known about role of and circuit formation vivo. We have reported involved neuron differentiation its overexpression ectopic cadherin-7 expression. this study we report also Tissue sections open book preparations...
Peripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach. Menstrual blood-derived endometrial cells (MenSCs) considered an ideal therapeutic option for peripheral repair due to noninvasive collection procedure their high proliferation rate immunological tolerance. Here, we successfully isolated MenSCs examined biological characteristics including morphology, multipotency,...
SUMMARY Brain regeneration requires a precise coordination of complex responses in time- and region-specific manner. Identifying key cell types molecules that direct brain would provide potential targets for the advance regenerative medicine. However, progress field has been hampered largely due to limited capacity mammalian understanding process at both cellular molecular level. Here, using axolotl with extrodinary ability upon injury, SpaTial Enhanced REsolution Omics-sequencing...
The tetrapod salamander species axolotl (Ambystoma mexicanum) is capable of regenerating injured brain. For better understanding the mechanisms brain regeneration, it very necessary to establish a rapid and efficient gain-of-function loss-of-function approaches study gene function in Here, we optimize an electroporation-based method overexpress or knockout/knockdown target ependymal glial cells (EGCs) telencephalon. By orientating electrodes, were able achieve specific expression EGFP EGCs...
Abstract The Mexican axolotl (Ambystoma mexicanum) is a promising tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, during which their capacity lifespan gradually decline. However, system-level single-cell analysis of molecular characteristics in metamorphosed still lacking. Here, we developed RNA-seq method based on combinatorial hybridization to generate tissue-based transcriptomic atlas the adult axolotl. We performed gene...