A5064139736- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Acute Myeloid Leukemia Research
- Gene Regulatory Network Analysis
- Immune cells in cancer
- Traffic Prediction and Management Techniques
- Hematopoietic Stem Cell Transplantation
- Intensive Care Unit Cognitive Disorders
- Face recognition and analysis
- Traffic control and management
- Pluripotent Stem Cells Research
- Generative Adversarial Networks and Image Synthesis
- Transportation Planning and Optimization
- Cancer Cells and Metastasis
- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Automated Road and Building Extraction
- Mindfulness and Compassion Interventions
- Respiratory Support and Mechanisms
- Sepsis Diagnosis and Treatment
- Psychosomatic Disorders and Their Treatments
- Pharmaceutical studies and practices
- Ultrasound in Clinical Applications
- Immune Cell Function and Interaction
- Target Tracking and Data Fusion in Sensor Networks
Zhejiang University
2017-2025
Ruijin Hospital
2020-2025
First Affiliated Hospital Zhejiang University
2020-2024
Beijing University of Posts and Telecommunications
2022-2024
Shanghai Jiao Tong University
2020-2022
Committee on Publication Ethics
2022
Sun Yat-sen University
2016
Sun Yat-sen University Cancer Center
2016
Changsha University of Science and Technology
2008
Dalian University of Technology
2002
Abstract Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity different organisms, cross-species comparisons landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating comprehensive dataset > 2.6 million single cells, constructed a identified signatures common pathways that...
Highly aggressive gastric cancer (HAGC) is a characterized by bone marrow metastasis and disseminated intravascular coagulation (DIC). Information about the disease limited. Here we employed single-cell RNA sequencing to investigate peripheral blood mononuclear cells (PBMCs), aiming unravel immune response of patients toward HAGC. PBMCs from seven HAGC patients, six normal advanced (NAGC) five healthy individuals were analysed sequencing. The expression genes interest was validated bulk...
Abstract Background Acute myeloid leukemia (AML) is a fatal hematopoietic malignancy and has prognosis that varies with its genetic complexity. However, there been no appropriate integrative analysis on the hierarchy of different AML subtypes. Methods Using Microwell-seq, high-throughput single-cell mRNA sequencing platform, we analyzed cellular bone marrow samples from 40 patients 3 healthy donors. We also used single-molecule real-time (SMRT) to investigate clonal heterogeneity cells....
Objective Multi-organ failure frequently complicates sepsis, with lungs being the primary target. T helper (Th) cell activation and phenotypic imbalance among them contribute significantly to sepsis-associated lung injury. Additionally, complement system could regulate polarized phenotype of lymphocytes. Therefore, this study investigated effect C5a/C5a receptor (C5aR)/Peptidylarginine deiminase 4 (PAD4) on Th1/Th2 ratio in sepsis-induced
Tumor heterogeneity plays a pivotal role in tumor progression and resistance to clinical treatment. Single-cell RNA sequencing (scRNA-seq) enables us explore within cell population identify rare types, thereby improving our design of targeted therapeutic strategies. Here, we use pan-cancer pan-tissue single-cell transcriptional landscape reveal heterogeneous expression patterns malignant cells, precancerous as well cancer-associated stromal endothelial cells. We introduce deep learning...
Cell trans-differentiation offers a powerful means to manipulate cell identities. By exposing cells combination of small molecules (SMs), can be induced in simple and cost-effective manner. However, comprehensive atlas detailing chemical-induced across multiple fates has yet established. In this study, the underlying mechanisms is investigated constructed an in-depth single-cell process. The time-course trajectory demonstrated for mouse embryonic fibroblasts (MEFs) into lineages including...
Acute lung injury (ALI) is the of alveolar epithelial cells and capillary endothelial caused by various factors. Complement system pyroptosis have been proved to be involved in ALI, inhibition C5a/C5a receptor (C5aR) could alleviate ALI. This study aimed investigate whether C5a/C5aR protect against LPS-induced ALI via mediating pyroptosis. Rats were assigned into four groups: Control, LPS, LPS+W-54011 1mg/kg, 5mg/kg. Beas-2B pretreated with or without C5a W-54011, alone combination,...
Convolutional Neural Networks (CNN) based detectors perform well in face manipulation detection, but are still limited by redundant information. Some methods focus on blending boundary to localize regions, discarding a part of useless information like background image. But these contain deceptive such as facial regions without texture, which occupies resources and affects detection accuracy. Besides, left out some features useful for identification. Therefore, this paper proposes module...
Face forgery instances involving celebrities are on the rise, owing to ease with which their large quantity of videos may be accessible Internet, world leaders particularly. While current face manipulation detectors have achieved impressive results several open datasets, incorporate persons various identities, they show performance degradation these high-quality ones targeting at celebrities. What is more, online usually undergo compression processing, marking detection task harder. Besides,...
In order to explore the effective diagnosis method of gynecological acute abdomen, this paper takes hospital abdomen patients as samples and selects admitted be included in study. They are divided into transabdominal ultrasound group, intracavitary combined group. Moreover, uses mathematical statistics carry out sample statistics. The statistical data mainly include ectopic pregnancy, torsion ovarian tumor pedicle, suppurative salpingitis, fallopian tube, hemorrhagic pelvic inflammatory...
Survivors of intensive care unit (ICU) transfer to the common ward are often accompanied by psychological distress, negative emotions, fatigue, and sleep disturbances that affect recovery. Mindfulness-based stress reduction (MBSR) has achieved reliable results in improving physical mental health. However, no clinical study been conducted evaluate effects MBSR on fatigue quality patients who survived ICU were transferred general wards.This is a prospective randomized controlled trial (RCT)...
Identification of effective culture conditions to maintain and possibly expand human HSPCs in vitro is an important goal. Recent advances highlight the efficacy chemicals maintaining converting cell fates. We screened 186 found that a combination CHIR-99021, Forskolin OAC1 (CFO) maintained CD34-positive cells vitro. Efficiency system was characterized using flow cytometry for cells, colony-forming assay xeno-transplants. treated with this had enhanced expression HSPC markers increased...
Abstract Background Down syndrome (DS), which is characterized by various malfunctions, the most common chromosomal disorder. As DS population continues to grow and of those with live beyond puberty, early‐onset health problems have become apparent. However, cellular landscape molecular alterations not been thoroughly studied. Methods This study utilized single‐cell resolution techniques examine in humans mice, spanning seven distinct organs. A total 71 934 mouse 98 207 human cells were...
Summary The classical hematopoietic hierarchy, which is mainly built with fluorescence-activated cell sorting (FACS) technology, proves to be inaccurate in recent studies. Single RNA-seq (scRNA-seq) analysis provides a solution overcome the limit of FACS-based type definition system for dissection complex cellular hierarchy. However, large-scale scRNA-seq constrained by throughput and cost traditional methods. Here, we developed Microwell-seq, high-throughput low-cost platform using...
Abstract Despite extensive efforts to sequence different genomes, genetic models interpret gene regulation and cell fate decisions are lacking for most species. Here, we performed whole-body single-cell transcriptomic analysis of zebrafish, Drosophila , earthworm. We then mapped landscapes covering eight representative metazoan species study through evolution. With uniformly constructed cross-species datasets, developed a deep learning-based strategy, Nvwa, predict expression decipher cis...
BACKGROUND:This study analyzed the epidemiological characteristics and trends of trauma injuries in Ruijin Hospital North, Shanghai, China, feasibility methods to prevent trauma. MATERIAL AND METHODS:In this retrospective cross-sectional study, electronic databases North were searched for patients who experienced severe from 2013 2016. Characteristics analyzed, including mechanism, gender, reasons injury, injury-associated causes death. RESULTS:Of 17,093 during period, 11,165 (65.3%) male...