A5100698887- SARS-CoV-2 and COVID-19 Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- Diabetes and associated disorders
- Long-Term Effects of COVID-19
- Diabetes Treatment and Management
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Respiratory viral infections research
- Hormonal Regulation and Hypertension
- Birth, Development, and Health
- Pancreatitis Pathology and Treatment
- Diet, Metabolism, and Disease
- Cell death mechanisms and regulation
- Polydiacetylene-based materials and applications
- Immune responses and vaccinations
Chinese People's Liberation Army
2025
Army Medical University
2015-2025
Huazhong Agricultural University
2022-2024
Sichuan University
2024
West China Hospital of Sichuan University
2024
Bioscience (China)
2024
Anhui Medical University
2024
Dalian Medical University
2023-2024
First Affiliated Hospital of Dalian Medical University
2023-2024
Shenzhen Third People’s Hospital
2019-2023
Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid cells from patients varying of COVID-19 and healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the severe COVID-9. Moderate cases presence highly clonally expanded CD8+ T cells. This atlas microenvironment suggests potential mechanisms underlying pathogenesis recovery...
Abstract Understanding the mechanism that leads to immune dysfunction in severe coronavirus disease 2019 (COVID-19) is crucial for development of effective treatment. Here, using single-cell RNA sequencing, we characterized peripheral blood mononuclear cells (PBMCs) from uninfected controls and COVID-19 patients paired broncho-alveolar lavage fluid (BALF). We found a close association decreased dendritic (DCs) increased monocytes resembling myeloid-derived suppressor (MDSCs), which...
The liver plays a critical role in both immune defense and tolerance the body. liver-resident cells (LrICs) determine properties, but unique composition heterogeneity of these are incompletely understood. Here, we dissect diversity LrICs by comprehensive transcriptomic profiling using unbiased single-cell RNA-sequencing (scRNA-seq). A total 70, 706 CD45+ from paired perfusion, spleen peripheral blood as references were profiled. We identified more than 30 discrete cell populations comprising...
Abstract The novel coronavirus SARS-CoV-2, etiological agent of recently named Coronavirus infected disease (COVID-19) by WHO, has caused more than 2, 000 deaths worldwide since its emergency in Wuhan City, Hubei province, China, December, 2019. symptoms COVID-19 varied from modest, mild to acute respiratory distress syndrome (ARDS), and the latter which is generally associated with deregulated immune cytokine production; however, we currently know little as interplay between extent clinical...
Abstract Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity different organisms, cross-species comparisons landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating comprehensive dataset > 2.6 million single cells, constructed a identified signatures common pathways that...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease 2019 (COVID-19), has seriously threatened global public health. COVID-19 been reported to be associated with an impaired IFN response. However, the mechanisms of how SARS-CoV-2 antagonizes host response are poorly understood. In this study, we report that helicase NSP13 inhibits type I production by directly targeting TANK-binding kinase 1 (TBK1) for degradation. Interestingly, inhibition autophagy...
Non-structural proteins (NSPs) play a crucial role in the replication of severe acute respiratory syndrome coronavirus 2, facilitating virus amplification and propagation. In this study, we conducted comprehensive investigation into stability all subunits comprising RNA-dependent RNA polymerase complex. Notably, our results reveal for first time that NSP8 is relatively unstable protein, which found to be readily recognized degraded by proteasome. This degradation process mediated host E3...
Abstract Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the ongoing global pandemic that poses substantial challenges to public health worldwide. A subset of COVID-19 patients experience systemic inflammatory response, known as cytokine storm, which may lead death. Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) an important mediator inflammation and cell Here, we examined interaction RIPK1-mediated innate...
Introduction Since December 2019, an outbreak of new coronavirus disease (coronavirus 2019 [COVID-19]) has developed into a world pandemic (1). The city Wuhan in China, as one the most seriously affected areas, is now out woods (2), and aggressive quarantine performed by government played crucial role. COVID-19 insidious highly contagious, it prone to cause cluster outbreaks (3,4). patients dialysis centers are relatively dense mobile, particularity with uremia who require long-term...
Severe coronavirus disease 2019 (COVID-19) is often indicated by lymphopenia and increased myelopoiesis; however, the underlying mechanism still unclear, especially alteration of hematopoiesis. It important to explore what extent how hematopoietic stem cells contribute impairment peripheral lymphoid myeloid compartments in COVID-19 patients. In this study, we used single-cell RNA sequencing assess bone marrow mononuclear from patients with blood as control. The results showed that these were...
Abstract The Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) is a global pandemic that seriously threatens health and socioeconomic development, but the existed antiviral drugs vaccines still cannot yet halt spread of epidemic. Therefore, comprehensive profound understanding pathogenesis SARS-CoV-2 urgently needed to explore effective therapeutic targets. Here, we conducted multiomics study SARS-CoV-2-infected lung epithelial cells, including...
Mycobacterium tuberculosis (Mtb) infection, including active (TB) and latent Mtb infection (LTBI), leads to diverse outcomes owing different host immune responses. However, the mechanisms that govern progression from LTBI TB remain poorly defined in humans. Here, we profiled lung cell populations within bronchoalveolar lavage fluid (BALF) patients with or using single-cell RNA sequencing (scRNA-seq). We found substantially changed compartments BALF, especially for three subsets of...
Abstract Improved vaccination requires better delivery of antigens and activation the natural immune response. Here we report a lipid nanoparticle system with capacity to carry antigens, including mRNA proteins, which is formed into virus-like structure by surface decoration spike demonstrating application against SARS-CoV-2 variants. The strategy uses S1 protein from Omicron BA.1 on deliver XBB.1. particle enables specific augmentation mRNAs expressed in human respiratory epithelial cells...
Tumors are comprised of malignant cancer cells and stromal which constitute the tumor microenvironment (TME). Previous studies have shown that associated fibroblast (CAF) in TME is an important promoter initiation progression. However, underlying molecular mechanisms by CAFs influence growth colorectal (CRCs) not been clearly elucidated. In this study, using a non-contact co-culture system between human fibroblasts (CCD-18-co) CRCs (LoVo, SW480, SW620), we found existing positively...
beta-Cell replacement therapy via islet transplantation is a promising possibility for the optimal treatment of type 1 diabetes; however, such an approach severely limited by shortage donor organs. This problem could be overcome if it were possible to generate transplantable islets from stem cells. We showed previously that adult beta-cells might originate duct or duct-associated Ductal progenitor cells in pancreas would become particularly useful therapies target beta-cell diabetic...
Abstract SARS-CoV-2 outbreak has been declared by World Health Organization as a worldwide pandemic. However, there are many unknowns about the antigen-specific T-cell-mediated immune responses to infection. Here, we present both single-cell TCR-seq and RNA-seq analyze dynamics of TCR repertoire metabolic functions blood T cells collected from recently discharged COVID-19 patients. We found that while diversity was increased in patients, it returned basal level ~1 week after becoming...
Curcuma longa (turmeric) has a long history of use in Ayurvedic medicine as treatment for inflammatory conditions. The purpose the present study was to investigate preventive effects curcumin against acute pancreatitis (AP) induced by caerulein mouse and elucidate possible mechanism action.Curcumin (50 mg/kg/day) intraperitoneally injected Kun Ming male mice 6 days, followed injection induce AP. GW9662 (0.3 mg/kg), specific peroxisome proliferator-activated receptor gamma (PPARγ) antagonist,...