A5039496752- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Anaerobic Digestion and Biogas Production
- Membrane Separation Technologies
- Cell Image Analysis Techniques
- Radiomics and Machine Learning in Medical Imaging
- Nanoplatforms for cancer theranostics
- Wastewater Treatment and Nitrogen Removal
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Catalytic Processes in Materials Science
- Gene Regulatory Network Analysis
- Cancer-related molecular mechanisms research
- Histone Deacetylase Inhibitors Research
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Botany, Ecology, and Taxonomy Studies
- Biofuel production and bioconversion
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Chemical synthesis and alkaloids
Zhejiang University
2017-2025
Tianjin Medical University
2016-2025
Shenzhen Second People's Hospital
2024-2025
Chongqing University
2025
Shantou University
2024
University of Macau
2020-2024
The First People's Hospital of Xiaoshan District, Hangzhou
2024
Mindray (China)
2023-2024
Shenzhen University Health Science Center
2024
Ningbo College of Health Sciences
2024
Abstract Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity different organisms, cross-species comparisons landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating comprehensive dataset > 2.6 million single cells, constructed a identified signatures common pathways that...
Lack of immunogenicity cancer cells has been considered a major reason for their failure in induction tumor specific T cell response. In this paper, we present evidence that decitabine (DAC), DNA methylation inhibitor is currently used the treatment myelodysplastic syndrome (MDS), acute myeloid leukemia (AML) and other malignant neoplasms, capable eliciting an anti-tumor cytotoxic lymphocyte (CTL) response mouse EL4 model. C57BL/6 mice with established tumors were treated DAC (1.0 mg/kg body...
Cell types are the basic building units of multicellular life, with extensive diversities. The evolution cell is a crucial layer comparative biology but thus far not comprehensively studied. We define compendium atlases using single-cell RNA-seq (scRNA-seq) data from seven animal species and construct cross-species cell-type evolutionary hierarchy. present roadmap for origin diversity major categories find that muscle neuron cells conserved types. Furthermore, we identify transcription...
Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions molecular dynamics during tissue zebrafish remain poorly understood. Here, we utilized Microwell-seq analyze more than 250,000 single cells covering major types constructed systematic landscape. We revealed single-cell for 18 both embryo adult stages. Single-cell mapping of caudal fin unique characteristic blastema population key genetic regulation...
Aging increases the risk of liver diseases and systemic susceptibility to aging-related diseases. However, cell type-specific changes underlying mechanism aging in higher vertebrates remain incompletely characterized. Here, we constructed first single-nucleus transcriptomic landscape primate aging, which resolved gene expression fluctuation hepatocytes across three zonations detected aberrant cell-cell interactions between niche cells. Upon in-depth dissection this rich dataset, identified...
Highly aggressive gastric cancer (HAGC) is a characterized by bone marrow metastasis and disseminated intravascular coagulation (DIC). Information about the disease limited. Here we employed single-cell RNA sequencing to investigate peripheral blood mononuclear cells (PBMCs), aiming unravel immune response of patients toward HAGC. PBMCs from seven HAGC patients, six normal advanced (NAGC) five healthy individuals were analysed sequencing. The expression genes interest was validated bulk...
Chronic wound healing impairment is a significant complication in diabetes. Hydrogels that maintain moisture and enable sustained drug release have become prominent for enhancing chronic care. Particularly, hydrogels respond to reactive oxygen species (ROS) are sought-after their dual capacity mitigate ROS facilitate controlled delivery at the site. We strategically designed an ROS-responsive scavenging supramolecular hydrogel composed of simple hexapeptide Glu-Phe-Met-Phe-Met-Glu (EFM)....
There is compelling evidence that FoxP3+ regulatory T cells (Tregs) play a critical role in promoting tumor immune evasion. Our prior research demonstrated the expression of miR-125b-5p directly inhibits Tregs by targeting TNFR2 and FoxP3. Given significant therapeutic potential mesenchymal stromal/stem cell (MSC)-derived exosomes (MSC-EXO) cancer treatment, MSC-EXO augmenting anti-tumor immunotherapy through delivery remains unexplored. Nanoparticle tracking analysis (NTA) transmission...
As one of the best known cancer testis antigens, PRAME is overexpressed exclusively in germ line tissues such as well a variety solid and hematological malignant cells including acute myeloid leukemia. Therefore, has been recognized promising target for both active adoptive anti-leukemia immunotherapy. However, most patients with PRAME-expressing leukemia, antigen-specific CD8+ CTL response are either undetectable or too weak to exert immune surveillance presumably due inadequate antigen...
Abstract Background Acute myeloid leukemia (AML) is a fatal hematopoietic malignancy and has prognosis that varies with its genetic complexity. However, there been no appropriate integrative analysis on the hierarchy of different AML subtypes. Methods Using Microwell-seq, high-throughput single-cell mRNA sequencing platform, we analyzed cellular bone marrow samples from 40 patients 3 healthy donors. We also used single-molecule real-time (SMRT) to investigate clonal heterogeneity cells....