A5050334079- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Neurogenesis and neuroplasticity mechanisms
- Renal and related cancers
- Single-cell and spatial transcriptomics
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Liver physiology and pathology
- Ovarian function and disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Reproductive biology and impacts on aquatic species
- Nerve injury and regeneration
- Biomedical Ethics and Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Parkinson's Disease Mechanisms and Treatments
- Muscle Physiology and Disorders
Kunming University of Science and Technology
2016-2025
Yunnan Yingmao Biotechnology (China)
2012-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Ningxia Medical University
2024
Yunnan Provincial Science and Technology Department
2017-2023
Chinese Academy of Sciences
2004-2021
Anna Needs Neuroblastoma Answers
2021
University of Chinese Academy of Sciences
2021
Institute of Zoology
2007-2021
Hainan University
2020
Abstract Bone marrow-derived mesenchymal stem cells (MSCs) hold great promise for treating immune disorders because of their immunoregulatory capacity, but the mechanism remains controversial. As we show here, MSC-mediated immunosuppression varies among different species. Immunosuppression by human- or monkey-derived MSCs is mediated indoleamine 2,3-dioxygenase (IDO), whereas mouse utilize nitric oxide, under same culture conditions. When expression IDO and inducible oxide synthase (iNOS)...
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene or pathology seen human disease. Addressing these issues is necessary if are generate large animal models diseases using CRISPR/Cas9. Here target monkey dystrophin create that lead Duchenne muscular dystrophy (DMD), recessive...
Mammalian mesenchymal stem cells (MSCs) have been shown to be strongly immunosuppressive in both animal disease models and human clinical trials. We reported that the key molecule mediating immunosuppression by MSCs is species dependent: indoleamine 2,3-dioxygenase (IDO) inducible nitric oxide synthase (iNOS) mouse. In present study, we isolated from several mammalian species, each of a different genus, investigated involvement IDO iNOS during MSC-mediated immunosuppression. The...
Whether and how certain transposable elements with viral origins, such as endogenous retroviruses (ERVs) dormant in our genomes, can become awakened contribute to the aging process is largely unknown. In human senescent cells, we found that HERVK (HML-2), most recently integrated ERVs, are unlocked transcribe genes produce retrovirus-like particles (RVLPs). These RVLPs constitute a transmissible message elicit senescence phenotypes young which be blocked by neutralizing antibodies. The...
The transition from peri-implantation to gastrulation in mammals entails the specification and organization of lineage progenitors into a body plan. Technical ethical challenges have limited understanding cellular molecular mechanisms that underlie this transition. We established culture system enabled development cynomolgus monkey embryos vitro for up 20 days. Cultured underwent key primate developmental stages, including segregation, bilaminar disc formation, amniotic yolk sac cavitation,...
Studies of cultured embryos have provided insights into human peri-implantation development. However, detailed knowledge lineage development as well underlying mechanisms remains obscure. Using 3D-cultured embryos, herein we report a complete cell atlas the early post-implantation lineages and decipher cellular composition gene signatures epiblast hypoblast derivatives. In addition, develop an embryo-like assembloid (E-assembloid) by assembling naive hESCs extraembryonic cells....
Science & Society26 March 2018Open Access The power of synthetic biology for bioproduction, remediation and pollution control UN's Sustainable Development Goals will inevitably require the application molecular biotechnology on a global scale Víctor de Lorenzo Global Future Council Biotechnologies, World Economic Forum, Geneva, Switzerland Systems Biology Program, National Center Biotechnology CSIC, Madrid, Spain Search more papers by this author Kristala LJ Prather Department Chemical...
Large animals (non-human primates, livestock and dogs) are playing important roles in biomedical research, large serve as sources of meat milk. The recently developed programmable DNA nucleases have revolutionized the generation gene-modified that used for biological research. In this review, we briefly introduce recent advances nuclease-meditated gene editing tools, outline these tools' applications human disease modeling, regenerative medicine agriculture. Additionally, provide...
Abstract Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1 , gene with well-established role in cardiogenesis, controls regulatory network amnion. CRISPR/Cas9-targeting of results embryos which do not yield viable offspring, demonstrating critically required embryogenesis. On cellular level, mutant...
The third and fourth weeks of gestation in primates are marked by several developmental milestones, including gastrulation the formation organ primordia. However, our understanding this period is limited due to restricted access vivo embryos. To address gap, we developed an embedded 3D culture system that allows for extended ex utero cynomolgus monkey embryos up 25 days post-fertilization. Morphological, histological, single-cell RNA-sequencing analyses demonstrate cultured largely...
The primate frontal lobe (FL) is sensitive to aging-related neurocognitive decline. However, the aging-associated molecular mechanisms remain unclear. Here, using physiologically aged non-human primates (NHPs), we depicted a comprehensive landscape of FL aging with multidimensional profiling encompassing bulk and single-nucleus transcriptomes, quantitative proteome, DNA methylome. Conjoint analysis across these neuropathological layers underscores nuclear lamina heterochromatin erosion,...
Splice-switching antisense oligonucleotides (ASOs) and engineered U7 small nuclear ribonucleoprotein (U7 Sm OPT) are the most commonly used methods for exon skipping. However, challenges remain, such as limited organ delivery repeated dosing ASOs unknown risks of by-products produced by OPT. Here, we showed that circular RNAs (AS-circRNAs) can effectively mediate skipping in both minigene endogenous transcripts. We also a relatively higher efficiency at tested Dmd than AS-circRNA...
Abstract Blastoids—blastocyst-like structures created in vitro—emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities, typically below 30%, has limited their utility. Prior research predominantly utilized cells. In this work, we demonstrate efficient generation from induced pluripotent cells and somatic cell...