A5025025992- RNA Research and Splicing
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Toxic Organic Pollutants Impact
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- PARP inhibition in cancer therapy
- Product Development and Customization
- Heat shock proteins research
Nanjing Medical University
2016-2025
Inner Mongolia University of Technology
2022
Significance Infertility is a global problem that afflicts 15% of couples, and in 50% cases, the attributing factor linked to men. Among these infertile men, 18% specifically exhibit decreased motility sperm (asthenozoospermia). Sperm dependent on formation functioning flagellum, modified cilium used for locomotion. Cilia are present almost every cell vertebrates essential proper organ functioning. Defects cilia lead severe syndromic diseases, termed ciliopathies, affecting numerous tissues...
Mammalian spermatogenesis shows prominent chromatin and transcriptomic switches in germ cells, but it is unclear how such dynamics are controlled. Here we identify RNA helicase DDX43 as an essential regulator of the remodeling process during spermiogenesis. Testis-specific Ddx43 knockout mice show male infertility with defective histone-to-protamine replacement post-meiotic condensation defects. The loss its ATP hydrolysis activity by a missense mutation replicates phenotype global mice....
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary insufficiency incomplete or absent sexual maturation, respectively. The associated defects spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions Dax1 knockout mouse models provide limited insight into AHC/HH disease mechanisms, because exhibit more extensive abnormalities testicular development, including...
Circular RNAs (circRNAs), covalently closed continuous RNA loops, are generated from cognate linear through back splicing events, and alternative events may generate different circRNA isoforms at the same locus. However, challenges of reconstruction quantification alternatively spliced full-length circRNAs remain unresolved. On basis internal structural characteristics circRNAs, we developed CircAST, a tool to assemble transcripts estimate their expression by using multiple splice graphs....
During meiosis, telomere attachment to the inner nuclear envelope is required for proper pairing of homologous chromosomes and recombination. Here, we identified F-box protein 47 (FBXO47) as a regulator telomeric shelterin complex that specifically expressed during meiotic prophase I. Knockout Fbxo47 in mice leads infertility males. We found deficient spermatocytes are unable form complete synaptonemal complex. FBXO47 interacts with TRF1/2, disruption destabilizes TRF2, leading unstable slow...
Abstract Tubulin-based microtubule is a core component of flagella axoneme and essential for sperm motility male fertility. Structural components the have been well explored. However, how tubulin folding regulated in formation still largely unknown. Here, we report germ cell-specific co-factor CCT complex, STYXL1. Deletion Styxl1 results infertility defects flagella. Proteomic analysis -/- reveals abnormal downregulation flagella-related proteins including tubulins. The N-terminal...
Transcription factors of the Sox protein family contain a DNA-binding HMG box and are key regulators progenitor cell fate. Here, we report that expression Sox30 is restricted to meiotic spermatocytes postmeiotic haploids. mutant males sterile due spermiogenic arrest at early round spermatid stage. Specifically, in absence Sox30, proacrosomic vesicles fail form single acrosomal organelle, spermatids step 2-3. Although most progress through meiosis, accumulation diplotene indicates delayed or...
Abstract Embryo loss and pregnancy disorders are prevalent worldwide, with both conditions critically associated dysfunctioning gastrulation processes. Gastrulation post-gastrulation organogenesis crucial stages of embryonic development that establish the blueprint for body part formation. These processes involve sequential generation three germ layer cells primordial cells, as well assembly precursor tissues parts. However, due to ethical limitations studying human embryogenesis, a more...
Despite continuous expansion of the RNA binding protein (RBP) world, there is a lack systematic understanding RBPs in mammalian testis, which harbors one most complex tissue transcriptomes. We adapted interactome capture to mouse male germ cells, building an RBP atlas characterized by multiple layers dynamics along spermatogenesis. Trapping RNA-cross-linked peptides showed that glutamic acid-arginine (ER) patch, residue-coevolved polyampholytic element present coiled coils, enhances its host...
Abstract Oligoasthenozoospermia is a major cause of male infertility; however, its etiology and pathogenesis are unclear may be associated with specific gene abnormalities. This study focused on Tppp2 (tubulin polymerization promoting protein family member 2), whose encoded localizes in elongating spermatids at stages IV‐VIII the seminiferous epithelial cycle testis mature sperm epididymis. In human mouse sperm, vitro inhibition TPPP2 caused significantly decreased motility ATP content....
Circular RNAs (circRNAs) are a large class of mammalian RNAs. Several protein products translated by circRNAs have been reported to be involved in the development various tissues and systems; however, their physiological functions male reproduction yet not explored.Here, we report an endogenous circRNA (circRsrc1) that encodes novel 161-amino-acid which named Rsrc1-161aa through sequencing coupled with mass spectrometry analysis on mouse testicular tissues. Deletion mice impaired fertility...
•STK33 has two loss-of-function mutations in the patients of NOA.•Stk33-/KI male mice were sterile with defects sperm formation.•Fibrous sheath proteins AKAP3/AKAP4 are novel phosphorylation substrates STK33.•Sperm from Stk33−/− have oocyte activation. Spermatogenesis important for infertility; however, etiology and pathogenesis still unknown. Herein, we identified STK33 seven individuals non-obstructive azoospermia. Further functional studies these frameshift nonsense revealed that...
Advances in single-cell technology have enabled the detailed mapping of testicular cell transcriptomes, which is essential for understanding spermatogenesis. However, fragmented nature age-specific data from various literature sources has hindered comprehensive analysis. To overcome this, Human Testis Database (HumanTestisDB) was developed, consolidating multiple human sequencing datasets to address this limitation. Through extensive investigation, 38 unique types were identified, providing...
Protein kinase C (PKC) family is evolutionally conserved and involved in various signaling cascades all cells. Of the family, PRKCQ dominatingly expressed testis, however, its molecular functionality spermatogenesis male fertility remains unclear. To evaluate role of spermatogenesis, Prkcq knockout mice were generated using CRISPR/Cas9 system. Histological immunofluorescence assays by different markers employed to assess testicular cells variation. Sperm parameters analyzed computer-assisted...
Spermatogenesis is a complex process closely associated with the phosphorylation-orchestrated cell cycle. Elucidating phosphorylation-based regulations should advance our understanding of underlying molecular mechanisms. Here we present an integrative study phosphorylation events in testis. Large-scale phosphoproteome profiling adult mouse testis identified 17,829 sites 3955 phosphoproteins. Although only approximately half enriched by IMAC were also captured TiO2, both phosphoprotein data...
The assembly of primordial follicles in mammals represents one the most critical processes ovarian biology. It directly affects number oocytes available to a female throughout her reproductive life. Premature depletion contributes pathology primary insufficiency (POI). To delineate developmental trajectory and regulatory mechanisms during process, we performed RNA-seq on single germ cells from newborn (P0.5) ovaries. Three cell clusters were classified which corresponded three states (germ...
Spermatogenesis requires a large number of proteins to be properly expressed at certain stages, during which post-transcriptional regulation plays an important role. RNA-binding (RBPs) are key players in regulation, but only few RBPs have been recognized and preliminary explored their function spermatogenesis present. Here we identified new RBP tubby-like protein 2 (TULP2) found three potential deleterious missense mutations Tulp2 gene dyszoospermia patients. Therefore, the mechanism TULP2...