A5102768146- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Congenital heart defects research
- Cancer Immunotherapy and Biomarkers
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- Telomeres, Telomerase, and Senescence
- RNA Research and Splicing
- DNA Repair Mechanisms
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Autophagy in Disease and Therapy
- Helicobacter pylori-related gastroenterology studies
- Ophthalmology and Eye Disorders
- Sperm and Testicular Function
- Congenital Heart Disease Studies
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Genetic and Kidney Cyst Diseases
- Mitochondrial Function and Pathology
Nanjing Medical University
2016-2025
Nanjing Maternity and Child Health Care Hospital
2023
Suzhou Institute of Nano-tech and Nano-bionics
2023
Chinese Academy of Sciences
2023
Georgetown University Medical Center
2017-2021
Georgetown University
2017-2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2020
Suzhou Institute of Systems Medicine
2019-2020
Center for Global Health
2020
Faculty of Public Health
2015
Abstract Cancer-testis ( CT ) genes represent the similarity between processes of spermatogenesis and tumorigenesis. It is possible that their selective expression pattern can help identify driver in cancer. In this study, we integrate transcriptomics data from multiple databases systematically 876 new 19 cancer types. We explore relationship with testis-specific regulatory elements. propose extremely highly expressed (EECTGs) are potential drivers activated through epigenetic mechanisms....
Abstract RNAi therapy has undergone two stages of development, direct injection synthetic siRNAs and delivery with artificial vehicles or conjugated ligands; both have not solved the problem efficient in vivo siRNA delivery. Here, we present a proof-of-principle strategy that reprogrammes host liver genetic circuits to synthesis self-assembly into secretory exosomes facilitate through circulating exosomes. By combination different circuit modules, assembled are systematically distributed...
Chinese lung cancer patients have distinct epidemiologic and genomic features, highlighting the presence of specific etiologic mechanisms other than smoking. Here, we present a comprehensive landscape 149 non-small cell (NSCLC) cases identify 15 potential driver genes. We reveal that are specially characterized by not only highly clustered EGFR mutations but mutational signature (MS3, 33.7%), is associated with inflammatory tumor-infiltrating B lymphocytes (P = 0.001). The mutation rate...
Abstract Background Epigenetic alterations have been shown to contribute immensely human carcinogenesis. Dynamic and reversible N6‐methyladenosine (m6A) RNA modification regulates gene expression cell fate. However, the reasons for activation of KIAA1429 (also known as VIRMA, an methyltransferase) its underlying mechanism in lung adenocarcinoma (LUAD) remain largely unexplored. In this study, we aimed clarify oncogenic role tumorigenesis LUAD. Methods Whole‐genome sequencing transcriptome...
Inner dynein arm (IDA), composed of a series protein complex, is necessary to cilia and flagella bend formation beating. Previous studies indicated that defects IDA complex result in multiple morphological abnormalities the sperm flagellum (MMAF) male infertility. However, genetic causes molecular mechanisms IDAs need further exploration. Here we identified two loss-of-function variants WDR63 both MMAF non-obstructive azoospermia (NOA) affected cohorts. encodes an IDA-associated dominantly...
Cardiogenesis is a tightly regulated dynamic process through continuum of differentiation and proliferation events. Key factors pathways governing this remain incompletely understood. Here, we investigate mice hearts from embryonic day 10.5 to postnatal week 8 dissect developmental changes in phosphoproteome-, proteome-, metabolome-, transcriptome-encompassing cardiogenesis cardiac maturation. We identify mitogen-activated protein kinases as core involved transcriptional regulation by...
Neonatal heart undergoes metabolic conversion and cell cycle arrest preparing for the increased workload during adulthood. Herein, we report that neonatal ketone body elevation is a critical regulatory factor postnatal development. Through multiomics screening, found expression of 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), rate-limiting enzyme ketogenesis, was transiently induced by colostrum in heart. Hmgcs2 knockout caused mitochondrial maturation defects. Meanwhile, development...
Exposure to PM
// Kaipeng Xie 1, 2, * , Hongxia Ma Cheng Liang Wang 2 Na Qin Wei Shen Yayun Gu Caiwang Yan Kai Zhang Ningbin Dai Meng Zhu Shuangshuang Wu 3 Hui Juncheng Guangfu Jin Hongbing Zhibin Hu 1 Department of Epidemiology and Biostatistics, Collaborative Innovation Center Cancer Medicine, School Public Health, Nanjing Medical University, Nanjing, China Jiangsu Key Lab Biomarkers, Prevention Treatment, The First Affiliated Hospital These authors have contributed equally to this work Correspondence...
The aim of this article was to evaluate whether genetic variants in autophagy-related genes affect the overall survival (OS) non-small cell lung cancer (NSCLC) patients. We analyzed 14 single nucleotide polymorphisms (SNPs) core for OS 1,001 NSCLC Three promising SNPs ATG10 were subsequently annotated by expression quantitative trait loci (eQTL) and methylation (meQTL) analyses based on Genotype-Tissue Expression (GTEx) Cancer Genome Atlas (TCGA) datasets. observed that rs10514231, rs1864182...
N6-methyladenosine (m6A) is the most prevalent reversible RNA modification in mammalian transcriptome. It has recently been demonstrated that m6A crucial for male germline development. Fat mass and obesity-associated factor (FTO), a known demethylase, widely expressed human mouse tissues involved manifold biological processes diseases. However, function of FTO spermatogenesis fertility remains poorly understood. Here, we generated an Fto knockout model using CRISPR/Cas9-mediated genome...
Telomeres maintain chromosomal stability and integrity are crucial in carcinogenesis. Telomere length is implicated multiple cancer risk, but the results conflicting. Genome-wide association studies have identified several genetic loci associated with telomere Caucasians. However, roles of related variants on gastric development largely unknown. We conducted a case–control study including 1136 cases 1012 controls to evaluate associations between length, eight length-related Caucasians risk...
Abstract Piwi‐like RNA ‐mediated gene silencing 1 ( PIWIL ) has been identified as a novel extremely highly expressed cancer‐testis CT in lung adenocarcinoma. However, the exact function and mechanism of adenocarcinoma remains unclear. Herein, we sought to investigate role occurrence development We examined expression pattern The Cancer Genome Atlas TCGA samples, validated it by Real‐Time PCR RT ‐ additional 21 paired tissues 16 normal tissues. Subsequently, explored biological A549 H1299...
•STK33 has two loss-of-function mutations in the patients of NOA.•Stk33-/KI male mice were sterile with defects sperm formation.•Fibrous sheath proteins AKAP3/AKAP4 are novel phosphorylation substrates STK33.•Sperm from Stk33−/− have oocyte activation. Spermatogenesis important for infertility; however, etiology and pathogenesis still unknown. Herein, we identified STK33 seven individuals non-obstructive azoospermia. Further functional studies these frameshift nonsense revealed that...
Genome-wide association studies have identified thousands of genetic variants associated with non-small cell lung cancer (NSCLC), however, it is still challenging to determine the causal and improve disease risk prediction. Here, we applied massively parallel reporter assays perform NSCLC variant-to-function mapping at scale. A total 1249 candidate were evaluated, 30 potential within 12 loci identified. Accordingly, proposed three architectures underlying susceptibility: multiple in a single...
PIWI-interacting RNAs (piRNAs) are small noncoding that almost exclusively expressed in germ cells to silence harmful transposons maintain genome stability. PIWIL4 is guided by its associated piRNAs transposable elements, where it recruits the DNA methylation apparatus and instructs de novo methylation. Herein, we identified a missense variant of (c.805 C>T p.R269W) two infertile males. Homozygous male mice carrying orthologous knock-in displayed elevated element expression aberrant gene...
The type 2 transmembrane serine protease matriptase is involved in many pathophysiological processes probably via its enzymatic activity, which depends on the dynamic relationship between zymogen activation and inhibition. Matriptase shedding can prolong life of enzymatically active increase accessibility to substrates. We show here that occurs a de novo proteolytic cleavage at sites located SEA domain CUB domain. Point or combined mutations four positively charged amino acid residues region...