A5101939780- Cancer Genomics and Diagnostics
- Advanced Energy Technologies and Civil Engineering Innovations
- RNA modifications and cancer
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Genomic variations and chromosomal abnormalities
- Lung Cancer Treatments and Mutations
- interferon and immune responses
- Lung Cancer Research Studies
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Drug Solubulity and Delivery Systems
- Ferroptosis and cancer prognosis
- Plant Pathogens and Fungal Diseases
- Probiotics and Fermented Foods
- Single-cell and spatial transcriptomics
- Hydrogels: synthesis, properties, applications
- Vasculitis and related conditions
- IL-33, ST2, and ILC Pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Metal complexes synthesis and properties
- Mycotoxins in Agriculture and Food
- Autonomous Vehicle Technology and Safety
- Complement system in diseases
- RNA Research and Splicing
National Institutes for Food and Drug Control
2023-2024
Peking University
2024
Peking University Third Hospital
2024
China Pharmaceutical University
2024
Southern Medical University
2024
Soochow University
2016-2023
Beijing Institute of Nanoenergy and Nanosystems
2020-2021
Tsinghua University
2021
South China University of Technology
2020
Xiamen University
2018
Presented here is a genome sequence of an individual human. It was produced from ∼32 million random DNA fragments, sequenced by Sanger dideoxy technology and assembled into 4,528 scaffolds, comprising 2,810 bases (Mb) contiguous with approximately 7.5-fold coverage for any given region. We developed modified version the Celera assembler to facilitate identification comparison alternate alleles within this diploid genome. Comparison National Center Biotechnology Information human reference...
There is much interest in characterizing the variation a human individual, because this may elucidate what contributes significantly to person's phenotype, thereby enabling personalized genomics. We focus here on variants 'exome,' which set of exons genome, exome believed harbor functional variation. provide an analysis ∼12,500 that affect protein coding portion individual's genome. identified ∼10,400 nonsynonymous single nucleotide polymorphisms (nsSNPs) ∼15–20% are rare population. predict...
Small cell lung cancer (SCLC) is an aggressive disease with poor survival. A few sequencing studies performed on limited number of samples have revealed potential disease-driving genes in SCLC, however, much still remains unknown, particularly the Asian patient population. Here we conducted whole exome (WES) and transcriptomic primary tumors from 99 Chinese SCLC patients. Dysregulation tumor suppressor TP53 RB1 was observed 82% 62% patients, respectively, more than half patients (62%)...
Chinese lung cancer patients have distinct epidemiologic and genomic features, highlighting the presence of specific etiologic mechanisms other than smoking. Here, we present a comprehensive landscape 149 non-small cell (NSCLC) cases identify 15 potential driver genes. We reveal that are specially characterized by not only highly clustered EGFR mutations but mutational signature (MS3, 33.7%), is associated with inflammatory tumor-infiltrating B lymphocytes (P = 0.001). The mutation rate...
Sodium Sulfobutylether-β-cyclodextrin (SBE-β-CD) is a derivative of β-cyclodextrin, characterized by its stereo structure, which closely resembles truncated cone with hydrophobic internal cavity. The solubility insoluble substances within the cavity significantly enhanced, reducing contact between guest and environment. Consequently, SBE-β-CD frequently employed as co-solvent stabilizer. As research progresses, it has been observed that inclusion reversible competitive. Besides, some...
It is now clear that tyrosine kinases represent attractive targets for therapeutic intervention in cancer. Recent advances DNA sequencing technology provide the opportunity to survey mutational changes cancer a high-throughput and comprehensive manner. Here we report on sequence analysis of members receptor kinase (RTK) gene family genomes glioblastoma brain tumors. Previous studies have identified number molecular alterations glioblastoma, including amplification RTK epidermal growth factor...
The scraper conveyor plays the role of bearing, coal, shearer guiding and hydraulic support in fully mechanized mining face, is "backbone" whole set equipment. Its performance, reliability life are important guarantee for normal production face good technical economic results. As one most components central groove, choice materials overall wear resistance play a vital conveyor. middle groove mainly as running track chain, channel coal load. In actual use process, damage rate gang plate very...
Background Sepsis is a common critical condition that can lead to multiple organ injury. Sepsis-induced acute respiratory distress syndrome (ARDS) frequently an important cause of poor prognosis and associated with high mortality rates, despite existing therapeutic interventions. Neutrophil infiltration extracellular traps (NET) are implicated in lung injury (ALI) ARDS following sepsis. As circulating neutrophils infiltrate infected tissues, they come into direct contact vascular endothelial...
It is difficult to detect the nighttime lane lines which showed darker and uneven lighted. In order overcome these problems, a recognition method was proposed. Firstly, edge enhancement based on Laplacian used enhance pre-processing image's edges. Then, edges were detected by Canny Otsu algorithm straight within one third at bottom of image Hough transform. Finally, an inside line extraction proposed basis slope constraint. Thereby, aim marking realized. By experimenting with various...
Studies already revealed that some E3 ubiquitin ligases participated in the immune response after viral infection by regulating type I interferon (IFN) pathway. Here, we demonstrated signaling enhanced translocation of ETS1 to nucleus and promoter activity ligase DTX3L (deltex 3L) virus thus increased expression DTX3L. Further experiments suggested ubiquitinated TBK1 at K30 K401 sites on K63-linked ubiquitination was also necessary for mediating phosphorylation through binding with tyrosine...
TGF-β-centered epithelial-mesenchymal transition (EMT) is a key process involved in radiation-induced pulmonary injury (RIPI) and fibrosis. PIEZO1, mechanosensitive calcium channel, expressed myeloid cell has been found to play an important role bleomycin-induced Whether PIEZO1 related with EMT remains elusive. Herein, we that functional rat primary type II epithelial cells RLE-6TN cells. After irradiation, expression was increased lung alveolar line, which accompanied changes evidenced by...
Abstract Long non-coding RNAs (lncRNAs) play a crucial role in macrophage development but little is known about their asthma. Here, we investigated the of lncRNA lncTRPM2-AS asthma and found that participates promotion inflammation. Downregulation promoted apoptosis inhibited proliferation production cytokines including IL-1β, IL-4, IL-6, IL-10, TNF-α, TGF-β. RNA-immunoprecipitation mass spectrometry indicated protein TRPM2 interacted with both E3 ubiquitin ligase TRIM21. LncTRPM2-AS...
An aquo-cisplatin arsenite multidrug nanocomposite loaded with cisplatin and arsenic trioxide prodrugs was reported for overcoming drug resistance combination therapy.
We tested the hypothesis that co-coordinated up-regulation or down-regulation of several ovarian cell surface kinases may provide clues for better understanding disease and help in rational design therapeutic targets.We compared expression signature 69 normal epithelial cells (OSE), with OSE from patients at high risk cancer.Seven kinases, ALK, EPHA5, EPHB1, ERBB4, INSRR, PTK, TGFbetaR1 displayed a distinctive linear trend normal, highrisk, malignant epithelium. confirmed these results using...
Tumors are characterized by properties of genetic instability, heterogeneity, and significant oligoclonality. Elucidating this intratumoral heterogeneity is challenging but important. In study, we propose a framework, BubbleTree, to characterize the tumor clonality using next generation sequencing (NGS) data. BubbleTree simultaneously elucidates complexity biopsy, estimating cancerous cell purity, ploidy, allele-specific copy number, represents in an intuitive graph. We further developed...
Cell-free DNA (cfDNA) is gaining substantial attention from both biological and clinical fields as a promising marker for liquid biopsy. Many aspects of disease-related features have been discovered cfDNA high-throughput sequencing (HTS) data. However, there still lack integrative systematic tools HTS data analysis quality control (QC).Here, we propose cfDNApipe, an easy-to-use python package whole-genome (WGS) bisulfite (WGBS) analysis. It covers the entire pipeline data, including raw...
Abstract The MAT-C1 subline of the 13762 rat mammary adenocarcinoma has highly stable, branched microvilli and immobile cell surface receptors. A membrane- microfilament-associated 58-kDa protein (p58) in been implicated stabilization microfilament-membrane interactions. This is associated with a high M(r) glycoprotein complex containing (proto)oncogene p185neu other signal transduction components putative particle. Amino acid sequences were obtained from two trypsin peptides p58. Screening...