A5060027924- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Peptidase Inhibition and Analysis
- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Trace Elements in Health
- Fungal Infections and Studies
- Radiopharmaceutical Chemistry and Applications
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Chromium effects and bioremediation
- Enzyme Catalysis and Immobilization
- Metalloenzymes and iron-sulfur proteins
- Legume Nitrogen Fixing Symbiosis
- Enzyme Production and Characterization
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Polyamine Metabolism and Applications
- Protist diversity and phylogeny
- Microbial Metabolic Engineering and Bioproduction
- Polysaccharides and Plant Cell Walls
- Synthesis and Biological Evaluation
- Antimicrobial Peptides and Activities
Innsbruck Medical University
2016-2025
Universität Innsbruck
2016-2025
Friedrich Schiller University Jena
2018
Leiden University
2014
Vienna Biocenter
2008-2013
Christian Doppler Laboratory for Thermoelectricity
2013
In-Q-Tel
2012
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2009
University of Vienna
2008
University of California, Los Angeles
2004
The ability to acquire iron in vivo is essential for most microbial pathogens. Here we show that Aspergillus fumigatus does not have specific mechanisms the utilization of host sources. However, it functional siderophore-assisted mobilization and reductive assimilation systems, both which are induced upon deprivation. Abrogation assimilation, by inactivation high affinity permease (FtrA), has no effect on virulence a murine model invasive aspergillosis. In striking contrast, A. l-ornithine-N...
Siderophore biosynthesis by the highly lethal mould Aspergillus fumigatus is essential for virulence, but non-existent in humans, presenting a rare opportunity to strategize therapeutically against this pathogen. We have previously demonstrated that A. excretes fusarinine C and triacetylfusarinine capture extracellular iron, uses ferricrocin hyphal iron storage. Here, we delineate pathways of intra- siderophore show synthesizes developmentally regulated fourth siderophore, termed...
Abstract NPS6, encoding a nonribosomal peptide synthetase, is virulence determinant in the maize (Zea mays) pathogen Cochliobolus heterostrophus and involved tolerance to H2O2. Deletion of NPS6 orthologs rice (Oryza sativa) pathogen, miyabeanus, wheat (Triticum aestivum) Fusarium graminearum, Arabidopsis thaliana Alternaria brassicicola, resulted reduced hypersensitivity Introduction ortholog from saprobe Neurospora crassa Δnps6 strain C. restored wild-type H2O2, demonstrating functional...
Iron is essential for a wide range of cellular processes. Here we show that the bZIP-type regulator HapX indispensable transcriptional remodeling required adaption to iron starvation in opportunistic fungal pathogen Aspergillus fumigatus. represses iron-dependent and mitochondrial-localized activities including respiration, TCA cycle, amino acid metabolism, iron-sulfur-cluster heme biosynthesis. In agreement with impact on mitochondrial HapX-deficiency decreases resistance tetracycline...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSuccessive Deposition of Alternate Layers Polyelectrolytes and a Charged VirusYuri Lvov, Heinrich Haas, Gero Decher, Helmuth Moehwald, Albert Mikhailov, Boris Mtchedlishvily, Ekaterina Morgunova, VainshteinCite this: Langmuir 1994, 10, 11, 4232–4236Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November...
Aspergillus fumigatus, the most common airborne fungal pathogen of humans, employs two high-affinity iron uptake systems: mediated by extracellular siderophore triacetylfusarinine C and reductive assimilation. Furthermore, A. fumigatus utilizes intracellular siderophores, ferricrocin hydroxyferricrocin, to store iron. Siderophore biosynthesis, which is essential for virulence, repressed Here we show that this control GATA factor SreA. During iron-replete conditions, SreA deficiency partially...
Aspergillus fumigatus is a common mould whose spores are component of the normal airborne flora. Immune dysfunction permits developmental growth inhaled in human lung causing aspergillosis, significant threat to health form allergic, and life-threatening invasive infections. The success A. as pathogen unique among close phylogenetic relatives poorly characterised at molecular level. Recent genome sequencing several species provides an exceptional opportunity analyse fungal virulence...
Summary The filamentous ascomycete A. nidulans produces two major siderophores: it excretes triacetylfusarinine C to capture iron and contains ferricrocin intracellularly. In this study we report the characterization of siderophore biosynthetic genes, sidA encoding l ‐ornithine N 5 ‐monooxygenase sidC a non‐ribosomal peptide synthetase respectively. Disruption eliminated synthesis deletion completely blocked biosynthesis. Siderophore‐deficient strains were unable grow, unless growth medium...
The steady state of histone acetylation is established and maintained by multiple acetyltransferases deacetylases, this affects chromatin structure function. identification a maize complementary DNA encoding the chromatin-bound deacetylase HD2 reported. This protein was not homologous to yeast RPD3 transcriptional regulator. It expressed throughout embryo germination in correlation with proliferative activity cells. Antibodies against recombinant HD2-p39 immunoprecipitated native enzyme...
Iron is an essential but, in excess, toxic nutrient. Therefore, fungi evolved fine-tuned mechanisms for uptake and storage of iron, such as the production siderophores (low-molecular mass iron-specific chelators). In Aspergillus fumigatus, iron starvation causes extensive transcriptional remodeling involving two central transcription factors, which are interconnected a negative feed-back loop: GATA-factor SreA bZip-factor HapX. During sufficiency, represses uptake, including reductive...
Human tear lipocalin (TL; also known as Lcn1) is a secretory protein present in large amounts fluids that cover epithelial surfaces such tears and respiratory secretions. It supposed to act physiological scavenger of hydrophobic, potentially harmful molecules, but there evidence it inhibits bacterial growth. In the study, we reconsidered possibility TL might interfere with microbial growth by scavenging siderophores, described for human neutrophil gelatinase-associated (NGAL). Indeed, our...
Gliotoxin, and other related molecules, are encoded by multi-gene clusters biosynthesized fungi using non-ribosomal biosynthetic mechanisms. Almost universally described in terms of its toxicity towards mammalian cells, gliotoxin has come to be considered as a component the virulence arsenal Aspergillus fumigatus. Here we show that deletion single gene, gliT, cluster two A. fumigatus strains, rendered organism highly sensitive exogenous completely disrupted secretion. Addition glutathione...
Small non-protein-coding RNAs (ncRNAs) have systematically been studied in various model organisms from Escherichia coli to Homo sapiens . Here, we analyse the small ncRNA transcriptome pathogenic filamentous fungus Aspergillus fumigatus To that aim, experimentally screened for ncRNAs, expressed under growth conditions or during specific developmental stages, by generating a specialized cDNA library size-selected RNA species. Our screen revealed 30 novel candidates known classes such as...
The Aspergillus fumigatus sterol regulatory element binding protein (SREBP) SrbA belongs to the basic Helix-Loop-Helix (bHLH) family of transcription factors and is crucial for antifungal drug resistance virulence. latter phenotype especially striking, as loss results in complete virulence murine models invasive pulmonary aspergillosis (IPA). How fungal SREBPs mediate unknown, though it has been suggested that lack growth hypoxic conditions accounts attenuated To further understand role...
Sterol regulatory element binding proteins (SREBPs) are a class of basic helix-loop-helix transcription factors that regulate diverse cellular responses in eukaryotes. Adding to the recognized importance SREBPs human health, fungal pathogens Cryptococcus neoformans and Aspergillus fumigatus required for virulence susceptibility triazole antifungal drugs. To date, exact mechanism(s) behind role SREBP these observed phenotypes is not clear. Here, we report A. SREBP, SrbA, mediates regulation...
Destruction of the pulmonary epithelium is a major feature lung diseases caused by mould pathogen Aspergillus fumigatus. Although it widely postulated that tissue invasion governed fungal proteases, A. fumigatus mutants lacking individual or multiple enzymes remain fully invasive, suggesting concomitant requirement for other pathogenic activities during host invasion. In this study we discovered, and exploited, novel, non-invasive, phenotype in pH-responsive transcription factor PacC. Our...
Abstract The opportunistic fungal pathogen Aspergillus fumigatus is increasingly found as a coinfecting agent along with Pseudomonas aeruginosa in cystic fibrosis patients. Amongst the numerous molecules secreted by P. during its growth, phenazines constitute major class. usually four phenazines, pyocyanin (PYO), phenazine-1-carboxamide (PCN), 1-hydroxyphenazine (1-HP) and phenazine-1-carboxylic acid (PCA). These inhibited growth of A. but underlying mechanisms impact these on biology were...
Abstract Soilborne fungal pathogens cause devastating yield losses and are highly persistent difficult to control. During the infection process, these organisms must cope with limited availability of iron. Here we show that bZIP protein HapX functions as a key regulator iron homeostasis virulence in vascular wilt fungus Fusarium oxysporum. Deletion hapX does not affect uptake but causes derepression genes involved iron-consuming pathways, leading impaired growth under iron-depleted...