A5102871633- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Photochromic and Fluorescence Chemistry
- HIV Research and Treatment
- Synthesis of Indole Derivatives
- Advanced Proteomics Techniques and Applications
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Machine Learning in Bioinformatics
- Lipid Membrane Structure and Behavior
- Pharmacological Effects and Toxicity Studies
- HIV/AIDS drug development and treatment
- Sleep and Wakefulness Research
- S100 Proteins and Annexins
- Genetic Associations and Epidemiology
- Sleep and related disorders
- SARS-CoV-2 and COVID-19 Research
- Cancer therapeutics and mechanisms
- Obesity, Physical Activity, Diet
Sichuan Agricultural University
2024
Nanjing Medical University
2021-2024
Nanjing University of Chinese Medicine
2022-2024
Huazhong University of Science and Technology
2016-2023
Academy of State Administration of Grain
2016-2023
University of California, San Francisco
2018-2022
Sichuan University
2022
State Key Laboratory of Oral Diseases
2022
Union Hospital
2016-2022
Soochow University
2020
Introducing new chemical reactivity into proteins in living cells would endow innovative covalent bonding ability to for research and engineering vivo. Latent bioreactive unnatural amino acids (Uaas) can be incorporated react with target natural acid residues via proximity-enabled reactivity. To expand the diversity of amenable such vivo, a functionality that is biocompatible able multiple under physiological conditions highly desirable. Here we report genetic encoding...
Genetically introducing novel chemical bonds into proteins provides innovative avenues for biochemical research, protein engineering, and biotherapeutic applications. Recently, latent bioreactive unnatural amino acids (Uaas) have been incorporated to covalently target natural residues through proximity-enabled reactivity. Aryl fluorosulfate is particularly attractive due its exceptional biocompatibility multitargeting capability via sulfur(VI) fluoride exchange (SuFEx) reaction. Thus far,...
Innate lymphoid cells (ILCs) are tissue-resident lymphocytes that play critical roles in cytokine-mediated regulation of homeostasis and inflammation. However, relationships between their immune phenotypic characteristics HCC remain largely unexplored.We performed single-cell RNA sequencing analysis on sorted hepatic ILC from human patients with validated using flow cytometry, multiplex immunofluorescence staining, functional experiments. Moreover, we applied selection strategies to enrich...
Significance Chemical cross-linking mass spectrometry is a powerful method to identify protein interaction partners. The cross-links also provide approximate interresidue distances, which can help model the structures of complexes. However, current reagents react with only limited set amino acid residues and their very high reactivity induce between sites that are distant in native state interrogated proteins. We therefore developed “plant-and-cast” strategy, heterobifunctional cross-linker...
Expansion of the genetic code with unnatural amino acids (Uaas) has significantly increased chemical space available to proteins for exploitation. Due inherent limitation translational machinery and required compatibility biological settings, function groups introduced via Uaas date are restricted chemically inert, bioorthogonal, or latent bioreactive groups. To break this barrier, here we report a new strategy enabling specific incorporation biochemically reactive into proteins. A acid is...
Genetically introducing covalent bonds into proteins in vivo with residue specificity is affording innovative ways for protein research and engineering, yet latent bioreactive unnatural amino acids (Uaas) genetically encoded to date react one few natural residues only, limiting the variety of scope applications amenable this technology. Here we report genetic encoding (2R)-2-amino-3-fluoro-3-(4-((2-nitrobenzyl)oxy) phenyl) propanoic acid (FnbY) Escherichia coli mammalian cells. Upon...
Background Several previous cross‐sectional studies suggested that body roundness index (BRI) may be associated with cardiovascular disease (CVD). However, the association should further validated. Our study aimed to assess of BRI trajectories CVD among middle‐aged and older Chinese people in a longitudinal cohort. Methods Results A total 9935 participants from CHARLS (China Health Retirement Longitudinal Study) repeated measurements 2011 2016 were included. The identified by group‐based...
Abstract A safe and effective vaccine against human immunodeficiency virus type 1 (HIV‐1) is urgently needed to combat the worldwide AIDS pandemic, but still remains elusive. The fact that uncontrolled replication of an attenuated can lead regaining its virulence creates safety concerns precluding many vaccines from clinical application. We introduce a novel approach control HIV‐1 replication, which entails manipulation essential protein biosynthesis through unnatural amino acid...
Site-specific modification of proteins with functional molecules provides powerful tools for researching and engineering proteins. Here we report a new chemical conjugation method which photocages highly reactive but chemically selective moieties, enabling the use protein-inert amines protein modification. New amino acids FnbY FmnbY, bearing photocaged quinone methides (QMs), were genetically incorporated into Upon light activation, they generated QM, rapidly reacted amine derivatives. This...
We report the first systematic evolution and study of tRNA variants that are able to read a set UAGN (N = A, G, U, C) codons in genomically recoded E. coli strain lacks any endogenous in-frame sequences release factor 1. Through randomizing bases anticodon stem-loop followed by functional selection, we identified mutants with significantly improved decoding efficiency, which will augment current efforts on genetic code expansion through quadruplet decoding. found an extended loop extra...
A safe and effective human immunodeficiency virus type 1 (HIV-1) vaccine is urgently needed, but remains elusive. While HIV-1 live-attenuated can provide potent protection as demonstrated in rhesus macaque-simian model, the potential pathogenic consequences associated with uncontrolled replication preclude such from clinical applications. We investigated a novel approach to address this problem by controlling through an unnatural genetic switch that was based on amber suppression strategy....
Abstract Small‐molecule crosslinkers are invaluable for probing biomolecular interactions and crosslinking mass spectrometry. Existing chemical target only a small selection of amino acids, while conventional photo‐crosslinkers almost all residues non‐specifically, complicating data analysis. Herein, we report photocaged quinone methide (PQM)‐based that nine nucleophilic through Michael addition, including Gln, Arg, Asn, which inaccessible to existing crosslinkers. PQM were used in vitro,...
Sensory neuron membrane proteins (SNMPs) play important roles in insect chemoreception and SNMP1s have been reported to be essential detecting sex pheromones Drosophila some lepidopteran species. However, SNMPs for Cyrtotrachelus buqueti (Coleoptera: Curculionidae), a major pest of bamboo plantations, remain uncharacterized. In this study, novel SNMP gene, CbuqSNMP1b, from C. was functionally characterized. The expression CbuqSNMP1b significantly higher antennae than other tissues both sexes...
We demonstrated a general protection-deprotection strategy for the design of fluorescent protein biosensors through construction turn-on Hg2+ sensor. A combination engineering and unnatural amino acid mutagenesis was used. Unlike previously reported protein-based sensors that relied on binding to sulfhydryl group cysteine residues, well-established chemical reaction, oxymercuration, transformed into biological format incorporated our sensor design. This novel displayed good sensitivity...
By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to amber nonsense codon bacteria. We demonstrated that optimization anticodon recognition tRNA by led improved efficiency is acid-specific. The findings will facilitate creation an optimized system genetic
Large efforts have been devoted to genetic code engineering in the past decade, aiming for unnatural amino acid mutagenesis. Recently, an increasing number of studies were reported employ quadruplet codons encode acids. We and others demonstrated that decoding efficiency could be significantly enhanced by extensive tRNAs bearing extra nucleotide their anticodon loops. In this work, we report identification tRNA mutants derived from directed evolution efficiently decode a UAGA codon mammalian...