A5003751904- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Cancer-related molecular mechanisms research
- Enzyme Catalysis and Immobilization
- MicroRNA in disease regulation
- Click Chemistry and Applications
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Circular RNAs in diseases
- Glycosylation and Glycoproteins Research
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Biochemical and biochemical processes
- High Entropy Alloys Studies
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- High-Temperature Coating Behaviors
- Real-time simulation and control systems
- Advanced Biosensing Techniques and Applications
University of Nebraska–Lincoln
2015-2024
Binzhou Technician College
2024
The University of Texas Health Science Center at Houston
2023-2024
Tiangong University
2023-2024
Tianjin Special Equipment Supervision and Inspection Technology Research Institute
2024
Xi'an Aeronautical University
2022-2023
Soochow University
2018-2023
Nanjing Agricultural University
2023
Institute of Software
2022-2023
Ministry of Agriculture and Rural Affairs
2023
Genome-scale reconstructions of metabolism are computational species-specific knowledge bases able to compute systemic metabolic properties. We present a comprehensive and validated reconstruction the biotechnologically relevant bacterium Pseudomonas putida KT2440 that greatly expands computable predictions its states. The represents significant reactome expansion over available reconstructed bacterial networks. Specifically, iJN1462 (i) incorporates several hundred additional genes...
Abstract Strains of Escherichia coli were constructed and evaluated that synthesized cis,cis ‐muconic acid from d ‐glucose under fed‐batch fermentor conditions. Chemical hydrogenation the cis , in resulting fermentation broth has also been examined. Biocatalytic synthesis adipic glucose eliminates two environmental concerns characteristic industrial manufacture: use carcinogenic benzene benzene‐derived chemicals as feedstocks generation nitrous oxide a byproduct nitric catalyzed oxidation....
Aim: This study aimed to determine whether circular RNA (circRNA) molecules in peripheral blood mononuclear cells (PBMCs) could be used as novel non-invasive biomarkers for major depressive disorder (MDD). Materials & methods: Differentially expressed circRNAs were screened using an Arraystar Human CircRNA Array (which includes 13,617 human circRNAs) and qRT-PCR. Thirty MDD patients randomly selected retest the circRNA levels after 4-week 8-week antidepressant regimens. Results: Four...
We have utilized in vitro evolution to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins response a quadruplet codon both bacterial and mammalian cells. This approach will facilitate creation an optimized standardized system genetic using codons, which allow biosynthesis biopolymers that contain multiple building blocks.
Bacterial gene expression is orchestrated by numerous transcription factors (TFs). Elucidating how regulated fundamental to understanding bacterial physiology and engineering it for practical use. In this study, a machine-learning approach was applied uncover the genome-scale transcriptional regulatory network (TRN) in Pseudomonas putida KT2440, an important organism bioproduction. We performed independent component analysis of compendium 321 high-quality profiles, which were previously...
The lack of a route to precursor 1,2,4-butanetriol that is amenable large-scale synthesis has impeded substitution trinitrate for nitroglycerin. To identify an alternative the current commercial racemic d,l-1,2,4-butanetriol involving NaBH4 reduction esterified d,l-malic acid, microbial syntheses d- and l-1,2,4-butanetriol have been established. These rely on creation biosynthetic pathways do not exist in nature. Oxidation d-xylose by Pseudomonas fragi provides d-xylonic acid 70% yield....
Tyrosine 66 of a green fluorescent protein (GFP) was substituted with unnatural amino acids carrying boronate, azido, nitro, and keto substituents. In general, the values these GFP mutants is blue-shifted relative to that GFP, fluorescence intensity boronate variant increases upon oxidation (see scheme). The X-ray crystal structures provide explanations their altered properties. Detailed facts importance specialist readers are published as "Supporting Information". Such documents...
MicroRNAs (miRNA, miR) have been implicated as promising blood‐based biomarkers for schizophrenia patients. This study aimed to clinically validate miRNA potential biomarkers. Plasma levels of 10 miRNAs were analyzed using qPCR in a cohort 61 patients and 62 normal controls, well 25 particularly selected six‐week antipsychotic treatment course. Positive And Negative Syndrome Scale (PANSS), Global Assessment (GAS) Clinical Impression (CGI) administered assess the clinical symptoms. The...
Background:The criteria for diagnosing depression are based on behavioral observation and self-reporting of symptoms by the patients or guardians without any biological validation disease.This study aimed to identify long non-coding RNAs (lncRNAs) in peripheral blood mononuclear cells (PBMCs) as robust predictive biomarkers diagnosis therapy response major depressive disorder (MDD). Material/Methods:We used human lncRNA 3.0 microarray profiling (which covers 30,586 lncRNAs), using PBMCs from...
Abstract A safe and effective vaccine against human immunodeficiency virus type 1 (HIV‐1) is urgently needed to combat the worldwide AIDS pandemic, but still remains elusive. The fact that uncontrolled replication of an attenuated can lead regaining its virulence creates safety concerns precluding many vaccines from clinical application. We introduce a novel approach control HIV‐1 replication, which entails manipulation essential protein biosynthesis through unnatural amino acid...
Abstract 1,2,4-Butanetriol (BT) is a valuable chemical with extensive applications in many different fields. The traditional routes to synthesize BT suffer from drawbacks, e.g., harsh reaction conditions, multiple steps and poor selectivity, limiting its industrial production. In this study, an engineered Escherichia coli strain was constructed produce xylose, which major component of the lignocellulosic biomass. Through coexpression xylose dehydrogenase ( CCxylB ) xylonolactonase xylC...
A new fluorogenic bioorthogonal reaction between styrene (an unstrained alkene) and a tetrazine was developed.
We report the first systematic evolution and study of tRNA variants that are able to read a set UAGN (N = A, G, U, C) codons in genomically recoded E. coli strain lacks any endogenous in-frame sequences release factor 1. Through randomizing bases anticodon stem-loop followed by functional selection, we identified mutants with significantly improved decoding efficiency, which will augment current efforts on genetic code expansion through quadruplet decoding. found an extended loop extra...
BACKGROUND Dysfunction of long non-coding RNAs (lncRNAs) has been demonstrated to be involved in psychiatric diseases. However, the expression patterns and functions regulatory lncRNAs schizophrenia (SZ) patients have rarely systematically reported. MATERIAL AND METHODS The peripheral blood mononuclear cells (PBMCs) were screened compared between SZ demographically-matched healthy controls using microarray analysis, then validated by quantitative real-time reverse transcription polymerase...
A safe and effective human immunodeficiency virus type 1 (HIV-1) vaccine is urgently needed, but remains elusive. While HIV-1 live-attenuated can provide potent protection as demonstrated in rhesus macaque-simian model, the potential pathogenic consequences associated with uncontrolled replication preclude such from clinical applications. We investigated a novel approach to address this problem by controlling through an unnatural genetic switch that was based on amber suppression strategy....
Abstract Carboxylic acid reductases (CARs) catalyze the reduction of a broad range carboxylic acids into aldehydes, which can serve as common biosynthetic precursors to many industrial chemicals. This work presents systematic biochemical characterization five from different microorganisms, including two known and three new ones, by using panel short‐chain dicarboxylic hydroxy acids, are cellular metabolites. All enzymes displayed substrate specificities. Higher catalytic efficiencies were...