Genome-scale reconstructions of metabolism are computational species-specific knowledge bases able to compute systemic metabolic properties. We present a comprehensive and validated reconstruction the biotechnologically relevant bacterium Pseudomonas putida KT2440 that greatly expands computable predictions its states. The represents significant reactome expansion over available reconstructed bacterial networks. Specifically, iJN1462 (i) incorporates several hundred additional genes...

Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain able to grow in presence of 90% and tolerant organic solvents whose log P(ow) (octanol/water partition coefficient) higher than 2.3. Solvent tolerance inducible, bacteria grown absence required an adaptation period before growth restarted. Mg2+ ions culture improved solvent tolerance. Electron micrographs showed that cells growing high concentrations exhibited...

The development of tolerance in Pseudomonas putida DOT-T1 to toluene and related highly toxic compounds involves short- long-term responses. short-term response is based on an increase the rigidity cell membrane by rapid transformation fatty acid cis-9,10-methylene hexadecanoic (C17:cyclopropane) unsaturated 9-cis-hexadecenoic (C16:1,9 cis) subsequent trans isomer. addition changes acids, alterations level phospholipid polar head groups: cardiolipin increases phosphatidylethanolamine...

ABSTRACT In this study, we show that glucose catabolism in Pseudomonas putida occurs through the simultaneous operation of three pathways converge at level 6-phosphogluconate, which is metabolized by Edd and Eda Entner/Doudoroff enzymes to central metabolites. When enters periplasmic space specific OprB porins, it can either be internalized into cytoplasm or oxidized gluconate. Glucose transported a process mediated an ABC uptake system encoded open reading frames PP1015 PP1018 then...

ABSTRACT In Pseudomonas putida DOT-T1E multidrug efflux pumps of the resistance-nodulation-division family make a major contribution to solvent resistance. Two have been identified: TtgABC, expressed constitutively, and TtgDEF, induced by aromatic hydrocarbons. A double mutant lacking both was able survive sudden toluene shock if only preinduced with small amounts supplied via gas phase. this article we report identification characterization in strain third pump, named TtgGHI. The ttgGHI...

The basic mechanisms underlying solvent tolerance in Pseudomonas putida DOT-T1E are efflux pumps that remove the from bacterial cell membranes. solvent-tolerant P. grows presence of high concentrations (e.g., 1% [vol/vol]) toluene and octanol. Growth cells LB supplied via gas phase has a clear effect on survival: sudden addition 0.3% (vol/vol) to pregrown with resulted survival almost 100% initial number, whereas only 0.01% absence tolerated exposure this aromatic hydrocarbon. One class...

Summary Maize represents one of the main cultivar for food and energy crop yields are influenced by soil physicochemical climatic conditions. To study how maize plants influence microbes we have examined microbial communities that colonize grown in carbonate‐rich ( pH 8.5) using culture‐independent, PCR ‐based methods. We observed a low proportion unclassified bacteria this whether it was planted or unplanted. Our results indicate higher complexity bacterial community is present bulk with...

Pseudomonas putida KT2440 is a chloramphenicol-resistant bacterium that able to grow in the presence of this antibiotic at concentration up 25 μg/ml. Transcriptomic analyses revealed expression profile 102 genes changed response chloramphenicol culture medium. The showed altered include those involved general metabolism, cellular stress response, gene regulation, efflux pump transporters, and protein biosynthesis. Analysis genome-wide collection mutants survival knockout mutant TtgABC...

Pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. In this study we describe the multilocus sequence typing four P. (HB13667, HB8234, HB4184, HB3267) isolated from in-patients at Besançon Hospital (France). The isolates (in particular were resistant to a number antibiotics. pathogenicity virulence potential was tested ex vivo using different biological models: tissue culture,...

A bacterium, Pseudomonas sp. strain C1S1, able to grow on 2,4,6-trinitrotoluene (TNT), 2,4- and 2,6-dinitrotoluene, 2-nitrotoluene as N sources, was isolated. The bacterium grew at 30 degrees C with fructose a source accumulated nitrite. Through batch culture enrichment, we isolated derivative strain, called clone A, which faster TNT did not accumulate nitrite in the medium. Use of by these two strains an involved successive removal nitro groups yield 2-nitrotoluene, toluene. Transfer putida...

Summary P seudomonas putida BIRD ‐1 is a plant growth‐promoting rhizobacterium whose genome size 5.7 Mbp. It adheres to roots and colonizes the rhizosphere high cell densities even in soils with low moisture. This property linked its ability synthesize trehalose, since mutant deficient synthesis of trehalose exhibited less tolerance desiccation than parental strain. The encodes wide range proteins that help it deal reactive oxygen stress generated rhizosphere. rhizobacteria properties derive...

Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the profiles animal human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil water but have been seldom isolated from humans. We established a collection P. in-patients different hospitals France. One (HB3267) kills insects is resistant to majority antibiotics used laboratories hospitals, including aminoglycosides, ß-lactams, cationic peptides,...

We report the identification of McpS as specific chemoreceptor for 6 tricarboxylic acid (TCA) cycle intermediates and butyrate in Pseudomonas putida. The analysis bacterial mutant deficient mcpS complementation assays demonstrate that is only TCA strain under study. are abundantly present root exudates, taxis toward these compounds proposed to facilitate access carbon sources. has an unusually large ligand-binding domain (LBD) un-annotated InterPro predicted contain helices. ligand profile...

Summary Bacterial chemotaxis is an adaptive behaviour, which requires sophisticated information‐processing capabilities that cause motile bacteria to either move towards or flee from chemicals. Pseudomonas putida DOT‐T1E exhibits the capability different aromatic hydrocarbons present at a wide range of concentrations. The chemotactic response mediated by McpT chemoreceptor encoded pGRT1 megaplasmid. Two alleles mcpT are borne on this plasmid and inactivation one led loss phenotype. Cloning...

In Gram-negative bacteria, multidrug efflux pumps are responsible for the extrusion of chemicals that deleterious growth. Some these induced by endogenously produced effectors, while abiotic or biotic signals induce expression other pumps. Pseudomonas putida, TtgABC pump is main antibiotic system respond to exogenous antibiotics through modulation this operon mediated TtgR. The plasmid-encoded TtgGHI in P. putida plays a minor role resistance parental strain; however, its critical isogenic...

Pseudomonas putida are strict aerobes that proliferate in a range of temperate niches and interest for environmental applications due to their capacity degrade pollutants ability promote plant growth. Furthermore solvent-tolerant strains useful biosynthesis added-value chemicals. We present comprehensive comparative analysis nine the first characterization pangenome. The core genome P. comprises approximately 3386 genes. most abundant genes within those encode nutrient transporters. Other...

Pseudomonas putida DOT‐T1E grows on a water–toluene double liquid phase. Toluene tolerance in this microorganism is mainly achieved by at least two efflux pumps that belong to the RND family. The TtgDEF pump induced toluene, whereas other pump, called TtgABC, expressed high level cells not exposed toluene and lower grown with toluene. ttgR gene adjacent ttgABC operon transcribed divergently from ttgA . expression of was fourfold higher growing presence than its absence. In TtgR‐deficient...

Pseudomonas putida EEZ15(pWW0-EB62) is a phosphinothricin (PPT)-resistant strain with recombinant TOL plasmid which allows the to grow on p-ethylbenzoate. The survival of this in sterile agricultural soils depends physicochemical properties soil. pWW0-EB62 and its catabolic functions were stable for periods up 1 month bacteria introduced unamended only conferred selective advantage host without or natural pWW0 when amended low amounts addition aromatics that are cometabolized by P. had...