Summary Maize represents one of the main cultivar for food and energy crop yields are influenced by soil physicochemical climatic conditions. To study how maize plants influence microbes we have examined microbial communities that colonize grown in carbonate‐rich ( pH 8.5) using culture‐independent, PCR ‐based methods. We observed a low proportion unclassified bacteria this whether it was planted or unplanted. Our results indicate higher complexity bacterial community is present bulk with...

Pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. In this study we describe the multilocus sequence typing four P. (HB13667, HB8234, HB4184, HB3267) isolated from in-patients at Besançon Hospital (France). The isolates (in particular were resistant to a number antibiotics. pathogenicity virulence potential was tested ex vivo using different biological models: tissue culture,...

The use of biological control agents (BCA), alone or in combination with other management measures, has gained attention over the past decades, driven by need to seek for sustainable and eco-friendly alternatives confront plant pathogens. rhizosphere olive (Olea europaea L.) plants is a source bacteria potential as biocontrol tools against Verticillium wilt (VWO) caused dahliae Kleb. A collection bacterial isolates from healthy nursery-produced (cultivar Picual, susceptible VWO) was...

Abstract The use of fertilizers and pesticides to control plant diseases is widespread in intensive farming causing adverse effects together with the development antimicrobial resistance pathogens. As virulence many Gram‐negative phytopathogens controlled by N‐acyl‐homoserine lactones (AHLs), enzymatic disruption this type quorum‐sensing (QS) signal molecules, mechanism known as quorum quenching (QQ), has been proposed a promising alternative antivirulence therapy. In study, novel strain...

Summary P seudomonas putida BIRD ‐1 is a plant growth‐promoting rhizobacterium whose genome size 5.7 Mbp. It adheres to roots and colonizes the rhizosphere high cell densities even in soils with low moisture. This property linked its ability synthesize trehalose, since mutant deficient synthesis of trehalose exhibited less tolerance desiccation than parental strain. The encodes wide range proteins that help it deal reactive oxygen stress generated rhizosphere. rhizobacteria properties derive...

Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the profiles animal human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil water but have been seldom isolated from humans. We established a collection P. in-patients different hospitals France. One (HB3267) kills insects is resistant to majority antibiotics used laboratories hospitals, including aminoglycosides, ß-lactams, cationic peptides,...

Summary Indole‐3‐acetic acid (IAA) is the main naturally occurring auxin and produced by organisms of all kingdoms life. In addition to regulation plant growth development, IAA plays an important role in interaction between plants growth‐promoting phytopathogenic bacteria regulating bacterial gene expression physiology. We show here that metabolizing plant‐associated Pseudomonas putida isolate exhibits chemotaxis independent metabolism. found based on activity PcpI chemoreceptor heterologous...

Summary Pseudomonas putida KT2440 exhibits two formaldehyde dehydrogenases and formate dehydrogenase complexes that allow the strain to stoichiometrically convert into CO 2 . The tolerated up 1.5 mM died in presence of 10 mM. In 0.5 formaldehyde, a sublethal concentration this chemical, growth rate decreased by about 40% with respect absence toxicant. Transcriptomic analysis revealed response low concentrations, limited number genes (52) were upregulated. Based on function these it seems...

Summary We used a combination of in silico and large‐scale mutagenesis approaches to expand our current knowledge the genetic determinants by Pseudomonas putida KT2440 attach surfaces. first identified orthologues that have been annotated aeruginosa as potentially involved attachment. In this search 67 paired‐related genes P. were associated adhesion. To test potential role corresponding gene products adhesion, 37 knockout mutants KT2440, available Reference Culture Collection, analysed with...

Summary Petroleum waste sludges are toxic and dangerous that is why environmental protection agencies have declared their treatment top priority. Physicochemical treatments expensive environmentally unfriendly, while alternative biological less costly but, in general, work at a slower pace. An situ bioremediation rhizoremediation field‐scale trial was performed an area contaminated with oil refinery sludge under semiarid climate. The included the use of artificial consortium made up plant...

Summary P seudomonas putida BIRD ‐1 has the potential to be used for industrial production of butanol due its solvent tolerance and ability metabolize low‐cost compounds. However, strain two major limitations: it assimilates as sole carbon source concentrations above 1% (v/v) are toxic. With aim facilitating design use, a genome‐wide mini‐Tn 5 transposon mutant library was screened clones exhibiting increased sensitivity or deficiency in assimilation. Twenty‐one mutants were selected that...

ABSTRACT We report the complete sequence of 5.7-Mbp genome Pseudomonas putida BIRD-1, a metabolically versatile plant growth-promoting rhizobacterium that is highly tolerant to desiccation and capable solubilizing inorganic phosphate iron synthesizing phytohormones stimulate seed germination growth.

The use of biological control agents (BCAs) is interest within an integrated management strategy Verticillium wilt olive (VWO) caused by the soil-borne fungus dahliae Kleb. Previous studies have shown that root/rhizosphere healthy plants important reservoir microorganisms displaying biocontrol activity against VWO (i.e., Pseudomonas strains PICF7 and PIC141). Moreover, these BCAs are already adapted to ecological niche where they deployed. Three novel bacteria (strains PIC28, PIC73 PIC167)...

Pseudomonas putida are ubiquitous inhabitants of soils and clinical isolates this species have been seldom described. Clinical show significant variability in their ability to cause damage hosts because some them able modulate the host's immune response. In current study, comparisons between genomes different environmental strains P. were done identify genetic clusters shared by that not present isolates. We specific genes mostly on transposons, set exhibit high identity with found pathogens...

Summary P seudomonas putida DOT ‐ T1E is an organic solvent tolerant strain capable of degrading aromatic hydrocarbons. Here we report the genomic sequence (6 394 153 bp) and its metabolic atlas based on classification enzyme activities. The genome encodes for at least 1751 enzymatic reactions that account known pattern C , N S utilization by this strain. Based potential to thrive in presence solvents subclasses enzymes encoded genome, map can be drawn a number biotransformation deduced....

Forest fires pose a serious threat to countries in the Mediterranean basin, often razing large areas of land each year. After fires, soils are more likely erode and resilience is inhibited part by toxic aromatic hydrocarbons produced during combustion cellulose lignins. In this study, we explored use bioremediation rhizoremediation techniques for soil restoration field-scale trial protected ecosystem after controlled fire. Our strategy combined Pseudomonas putida strains, indigenous...

Abstract The plant microbiome is essential for fitness and health. Antibiotics produced by plant‐associated bacteria have been shown to play an important role in protecting hosts against phytopathogens. Here, we highlight the strong biotechnological potential of (i) antibiotic producing as biocontrol agents (ii) heterologous expression biosynthetic gene clusters non‐pathogenic bacteria. We also provide complete list active substances based on bacteria, fungi, viruses currently approved or...

Abstract Microorganisms are exposed in their natural niches to a wide diversity of signal molecules. Specific detection these signals results alterations microbial metabolism and physiology. Auxins like indole‐3‐acetic acid key phytohormones that regulate plant growth development. Nonetheless, auxin biosynthesis is not restricted plants but ubiquitous all kingdoms life. This phylogenetic distribution auxins production, together with the regulated cellular processes, have made intra‐...