A5071366275- Inflammatory Bowel Disease
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- IL-33, ST2, and ILC Pathways
- Enzyme function and inhibition
- ATP Synthase and ATPases Research
- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- T-cell and B-cell Immunology
- Liver Diseases and Immunity
- Protein Tyrosine Phosphatases
- Photosynthetic Processes and Mechanisms
- Vitamin K Research Studies
- Autoimmune and Inflammatory Disorders Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Systemic Lupus Erythematosus Research
- Celiac Disease Research and Management
- Microbial Metabolic Engineering and Bioproduction
- Whipple's Disease and Interleukins
- Immunotherapy and Immune Responses
- Diverticular Disease and Complications
Hospital Universitario Virgen de las Nieves
2007-2018
Stanford University
2004-2018
Groupe d'Étude Thérapeutique des Affections Inflammatoires du Tube Digestif
2018
Abengoa (Spain)
2015-2016
Carnegie Institution for Science
2008-2014
Azienda Ospedaliera Universitaria Pisana
2014
Basingstoke and North Hampshire Hospital
2014
Carnegie Department of Plant Biology
2008-2013
Instituto Tecnológico Superior de Irapuato
2013
Instituto de Investigación Biosanitaria de Granada
2011
Inorganic polyphosphate (poly P) is a polymer made from as few 10 to several hundred phosphate molecules linked by phosphoanhydride bonds similar ATP. Poly P ubiquitous in all mammalian organisms, where it plays multiple physiological roles. The metabolism of poly organisms not well understood. We have examined the mechanism production and role this cell energy metabolism. levels mitochondria intact cells were estimated using fluorescent molecular probe, 4',6-diamidino-2-phenylindole....
The aims of this study were to assess the risk relapse after discontinuation anti-tumor necrosis factor (anti-TNF) drugs in patients with inflammatory bowel disease (IBD), identify factors associated relapse, and evaluate overcome retreatment same anti-TNF those who relapsed.This was a retrospective, observational, multicenter study. IBD had been treated anti-TNFs whom these discontinued clinical remission achieved included.A total 1,055 included. incidence rate 19% 17% per patient-year...
Mitochondrial dysfunction caused by excessive Ca2+ accumulation is a major contributor to cardiac cell and tissue damage during myocardial infarction ischemia-reperfusion injury (IRI). At the molecular level, mitochondrial induced Ca2+-dependent opening of permeability transition pore (mPTP) in inner membrane, which leads dissipation membrane potential (ΔΨm), disruption adenosine triphosphate production, ultimately death. Although role for induction mPTP established, exact mechanism this...
The PTPN22 gene is an important risk factor for human autoimmunity. aim of this study was to evaluate the first time role R263Q polymorphism in ulcerative colitis (UC) and Crohn's disease (CD), reevaluate association R620W with both diseases.A total 1677 UC patients, 1903 CD 3111 healthy controls from initial case-control set Spanish Caucasian ancestry two independent sample sets European (Dutch New Zealand) were included study. Genotyping performed using TaqMan SNP assays (rs33996649)...
Summary Background Onset during old age has been reported in upto 10% of total cases inflammatory bowel disease ( IBD ). Aim To evaluate phenotypic characteristics and the use therapeutic resources patients with elderly onset . Methods Case‐control study including all those diagnosed over 60 years since 2000 who were followed‐up for >12 months, identified from databases. Elderly compared aged 18 to 40 at diagnosis, matched by year gender type (adult‐onset). Results One thousand three...
The genomes of two closely related thermophilic cyanobacterial isolates, designated Synechococcus isolate OS-A and OS-B', from the microbial mats Octopus Spring (Yellowstone National Park) have been sequenced. An extensive suite genes that are controlled by phosphate levels constitute putative Pho regulon in these cyanobacteria. We examined physiological responses an axenic OS-B' as well transcript abundances cells acclimated to phosphorus-limiting conditions. Upon imposition phosphorus...
A functional polymorphism located at -1 from the start codon of CD40 gene, rs1883832, was previously reported to disrupt a Kozak sequence essential for translation. It has been consistently associated with Graves' disease risk in populations different ethnicity and genetic proxies this variant evaluated genome-wide association studies have shown evidence an effect rheumatoid arthritis multiple sclerosis (MS) susceptibility. However, protective allele or role MS, that we aimed replicate...
Inorganic polyphosphate (polyP) is a highly charged polyanion capable of interacting with number molecular targets. This signaling molecule released into the extracellular matrix by central astrocytes and peripheral platelets during inflammation. While release polyP associated both induction blood coagulation astrocyte signaling, role secreted in regulation neuronal activity remains undefined. Here we test hypothesis that an important participant signaling. Specifically, investigate ability...
Inorganic polyphosphate (poly P), a chain of hundreds phosphate residues linked by ATP-like bonds, is found in every cell nature and commonly produced from ATP poly P kinases (e.g., PPK1). Dictyostelium discoideum , the social slime mold, possesses PPK activity (DdPPK1) with sequence similarity to bacterial PPKs. We find here previously unrecognized (DdPPK2) D. sequences properties actin-related proteins (Arps) that are similar muscle actins size, properties, globular-filamentous structural...
Polyphosphate kinase 1 (PPK1), the principal enzyme responsible for reversible synthesis of polyphosphate (poly P) from terminal phosphate ATP, is highly conserved in bacteria and archaea. Dictyostelium discoideum, a social slime mold, one few eukaryotes known to possess PPK1 homolog (DdPPK1). Compared with Escherichia coli, DdPPK1 contains residues ATP binding autophosphorylation, but has an N-terminal extension 370 aa, lacking homology any protein. or promote oligomerization vitro. The...
Summary P seudomonas putida BIRD ‐1 has the potential to be used for industrial production of butanol due its solvent tolerance and ability metabolize low‐cost compounds. However, strain two major limitations: it assimilates as sole carbon source concentrations above 1% (v/v) are toxic. With aim facilitating design use, a genome‐wide mini‐Tn 5 transposon mutant library was screened clones exhibiting increased sensitivity or deficiency in assimilation. Twenty‐one mutants were selected that...
Dictyostelium discoideum , a social slime mold that forms fruiting bodies with spores, depends on inorganic polyphosphate (poly P) for its cycles of development and nutritional predation bacteria. The synthesis poly P, polymer tens or hundreds phosphate residues linked by high energy, ATP-like bonds, is catalyzed in most bacteria P kinase (PPK1). eukaryote D. possesses homolog PPK1. We report here mutants PPK1 (DdPPK1) have reduced levels are deficient development. Fruiting smaller produce...
OBJECTIVES: Genome-wide association studies have reported the role of interleukin (IL)2-IL21chromosomal region at 4q27 in several autoimmune conditions. Mice deficient IL-2 develop a disease with clinical and histological similarity to ulcerative colitis (UC) humans. Modest evidence linkage UC was tentatively proposed for theIL2gene more than decade ago. Therefore, we decide investigate polymorphisms axis (IL2,IL2RA,andIL2RBgenes) inflammatory bowel diseases (IBDs). METHODS: Seven hundred...
Synechococcus OS-B', a thermophilic unicellular cyanobacterium, recently isolated from the microbial mats in Octopus Spring (Yellowstone National Park), induces suite of genes, including phosphatases and transporters, response to phosphorus (P) starvation. Here we describe two different approaches examine ability OS-B' synthesize break down polyphosphate (poly P), key storage compound many prokaryotes. First, developed transformation protocol create mutants kinase (ppk), major enzyme...
The aims of this study were to evaluate the frequency entero-urinary fistulas in a cohort Crohn's disease (CD) patients and analyse outcomes medical surgical therapy. This multicentre retrospective included all CD with diagnosed by presence clinical symptoms confirmed at surgery or radiological endoscopic techniques. We evaluated defined remission as absence confirmation fistula closure. Cox regression analysis was performed factors predictive achieving without need for surgery. Of 6081...